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The map label for this gene is mnmC [H]

Identifier: 161486815

GI number: 161486815

Start: 557697

End: 559697

Strand: Reverse

Name: mnmC [H]

Synonym: t0485

Alternate gene names: 161486815

Gene position: 559697-557697 (Counterclockwise)

Preceding gene: 29141003

Following gene: 29140994

Centisome position: 11.68

GC content: 57.87

Gene sequence:

>2001_bases
GTGAAACAATACGCCATACAACCCGCCACACTCGAATTTAACGCTGAGGGTACACCTGTTTCCCGAGATTTTGATGATGT
CTATTTTTCTAATGACAATGGACTCGAAGAGACACGCTACGTTTTTCTTGGCGGCAATCGTCTTGCGGAGCGATTTCCCG
TACATTCACATCCATTATTTATAGTCGCGGAAAGCGGTTTTGGCACCGGACTCAACTTCCTGACGCTGTGGCAGGCGTTT
GACAGCTTTCGTTCTGCGCACCCACAGGCGACGCTGCAAAGGTTACATTTCATCAGCTTTGAGAAATTCCCGTTAACGCG
CGACGATCTGGCGCTGGCGCACCAACACTGGCCGGAGCTCGCGCCCTGGGCAGAGCAGCTGCAGGCGCAATGGCCGCTGC
CGCTCCCGGGGTGCCACCGCCTATTGCTGGATCGGGGTCGGGTGACGCTGGATTTGTGGTTTGGCGATATTAATGAGCTG
ACCGACCAACTGGACGCCACGCTGAATCAAACAGTCGACGCCTGGTTCCTCGACGGTTTTGCCCCGGCGAAAAATCCGGA
TATGTGGACGCCAAACCTGTTTAACGCGATGGCGCGGCTGGCGCGGCCCGGCGCAACGCTGGCGACGTTTACCTCGGCGG
GTTTCGTCCGTCGGGGATTACAGGAAGCCGGTTTTACCATGCAAAAACACAAAGGCTTCGGACGTAAACGCGAGATGCTT
TGTGGAGTGATGGAACAACACCTCATGCCGACCCTTTCCGCGCCCTGGTTTTACTGCAGCGGAAGCGAAAAACGTGAAAC
GGCGATCATTGGCGGCGGCATCGCCAGCGCGCTGTTATCACTGGCGTTACTGCGTCGCGGCTGGCAGGTGACGCTCTATT
GCGCAGATGATCAGCCCGCCCAGGGCGCTTCCGGTAATCGACAGGGCGCGCTTTATCCGCTCCTTAGCAAACATGACGCC
GCCATTAATCGCTTTTTCCCGACCGCATTCACCTTTGCCCGTCGCCTGTATGATGCCCTGCCCGTCTCTTTTGATCATGC
CTGGTGCGGCGTGACACAGCTCGGATGGGATGAGAAAAGCCAGCAGAAAATTGCCCAGATGCTGTCCCTGGCGCTGCCCG
CCGGACTGGCCTCGGCGCTCAATGCCGAAGAGGCGGAACAGGCGGTTGGCGTGACGACACGCTGCGGAGGAATTACCTAT
CCGGCAGGCGGCTGGCTGTGCCCGGAACAATTAACTCGCGCAGTCATCGCACTGGCGACTGAACAGGGTTTACAAACGCG
CTTTCGCCATACGCTCACGTCGCTCGTTGCGCAGGAGTCGCGCTGGCAACTGCGCTTTACGTCAGGTGAAACCGCCAGCC
ATGAGACTGTCGTCCTGGCGAACGGCCATCAGATTAACCGTTTTGATCAGACCCGGCCGCTGCCGGTTTATGCCGTCGGG
GGGCAGGTCAGTCATATTCCCACCACCCCCGCCCTCTCGGCGTTGCGTCAGGTGTTATGCTACGACGGCTATCTGACGCC
GCAAAATCCGCATAATCAGCAGCACTGTATTGGCGCCAGCTATCACCGGGGCGACGAAAGCACCGTCTGGCGCGAAGAAG
ATCAGCGTCAGAACCGACAGCGTCTGCTTGACTGTTTTCCGGATGCGAACTGGGCCACAGAGGTTGACGTTAGCGGCAAC
AGCGCCCGTTGCGGCGTGCGTTGCGCCACCCGCGACCATCTGCCGATGGTGGGCAATGTCCCGGATTACCACGCCACGCT
GACGCATTACGCCGATTTAGCCGATAATAAAACGTCGGCGGCGTCTGCGCCTGTGTATCCGGGTTTATTTATGCTCGGCG
CGCTGGGATCGCGTGGTTTGTGTTCAGCGCCATTATGCGCAGAAATTCTGGCGGCGCAGATGAGTAACGAGCCGATTCCA
CTGGATGCCGGTACGCTGGCGGCGTTAAATCCAAATCGGTTATGGGTGAGAAAGTTATTGAAGGGAAAAGCGGTGAAGTA
A

Upstream 100 bases:

>100_bases
GTACGCCGAACAAGTCCGATCAGCCATTTAGTACGGAAATTTGCGCCATCAGGCACACATCGTTAAGATCGCGATACCGC
CCGTCAGACGAGTAACTTAC

Downstream 100 bases:

>100_bases
TGGGCCAGTCAATAGCGGAAGGCGGCAAGCGTCGCCTTCCGGTCCACGGCGTTATTGTTTTGCCTTGTCGAACAAATTGT
CCCACATCGCTTTGACTAAC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MKQYAIQPATLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLAERFPVHSHPLFIVAESGFGTGLNFLTLWQAF
DSFRSAHPQATLQRLHFISFEKFPLTRDDLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDRGRVTLDLWFGDINEL
TDQLDATLNQTVDAWFLDGFAPAKNPDMWTPNLFNAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKHKGFGRKREML
CGVMEQHLMPTLSAPWFYCSGSEKRETAIIGGGIASALLSLALLRRGWQVTLYCADDQPAQGASGNRQGALYPLLSKHDA
AINRFFPTAFTFARRLYDALPVSFDHAWCGVTQLGWDEKSQQKIAQMLSLALPAGLASALNAEEAEQAVGVTTRCGGITY
PAGGWLCPEQLTRAVIALATEQGLQTRFRHTLTSLVAQESRWQLRFTSGETASHETVVLANGHQINRFDQTRPLPVYAVG
GQVSHIPTTPALSALRQVLCYDGYLTPQNPHNQQHCIGASYHRGDESTVWREEDQRQNRQRLLDCFPDANWATEVDVSGN
SARCGVRCATRDHLPMVGNVPDYHATLTHYADLADNKTSAASAPVYPGLFMLGALGSRGLCSAPLCAEILAAQMSNEPIP
LDAGTLAALNPNRLWVRKLLKGKAVK

Sequences:

>Translated_666_residues
MKQYAIQPATLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLAERFPVHSHPLFIVAESGFGTGLNFLTLWQAF
DSFRSAHPQATLQRLHFISFEKFPLTRDDLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDRGRVTLDLWFGDINEL
TDQLDATLNQTVDAWFLDGFAPAKNPDMWTPNLFNAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKHKGFGRKREML
CGVMEQHLMPTLSAPWFYCSGSEKRETAIIGGGIASALLSLALLRRGWQVTLYCADDQPAQGASGNRQGALYPLLSKHDA
AINRFFPTAFTFARRLYDALPVSFDHAWCGVTQLGWDEKSQQKIAQMLSLALPAGLASALNAEEAEQAVGVTTRCGGITY
PAGGWLCPEQLTRAVIALATEQGLQTRFRHTLTSLVAQESRWQLRFTSGETASHETVVLANGHQINRFDQTRPLPVYAVG
GQVSHIPTTPALSALRQVLCYDGYLTPQNPHNQQHCIGASYHRGDESTVWREEDQRQNRQRLLDCFPDANWATEVDVSGN
SARCGVRCATRDHLPMVGNVPDYHATLTHYADLADNKTSAASAPVYPGLFMLGALGSRGLCSAPLCAEILAAQMSNEPIP
LDAGTLAALNPNRLWVRKLLKGKAVK
>Mature_666_residues
MKQYAIQPATLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLAERFPVHSHPLFIVAESGFGTGLNFLTLWQAF
DSFRSAHPQATLQRLHFISFEKFPLTRDDLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDRGRVTLDLWFGDINEL
TDQLDATLNQTVDAWFLDGFAPAKNPDMWTPNLFNAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKHKGFGRKREML
CGVMEQHLMPTLSAPWFYCSGSEKRETAIIGGGIASALLSLALLRRGWQVTLYCADDQPAQGASGNRQGALYPLLSKHDA
AINRFFPTAFTFARRLYDALPVSFDHAWCGVTQLGWDEKSQQKIAQMLSLALPAGLASALNAEEAEQAVGVTTRCGGITY
PAGGWLCPEQLTRAVIALATEQGLQTRFRHTLTSLVAQESRWQLRFTSGETASHETVVLANGHQINRFDQTRPLPVYAVG
GQVSHIPTTPALSALRQVLCYDGYLTPQNPHNQQHCIGASYHRGDESTVWREEDQRQNRQRLLDCFPDANWATEVDVSGN
SARCGVRCATRDHLPMVGNVPDYHATLTHYADLADNKTSAASAPVYPGLFMLGALGSRGLCSAPLCAEILAAQMSNEPIP
LDAGTLAALNPNRLWVRKLLKGKAVK

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=666, Percent_Identity=79.8798798798799, Blast_Score=1092, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 73598; Mature: 73598

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYAIQPATLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLAERFPVHSHPLF
CCCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHCCCCCCCEE
IVAESGFGTGLNFLTLWQAFDSFRSAHPQATLQRLHFISFEKFPLTRDDLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
APWAEQLQAQWPLPLPGCHRLLLDRGRVTLDLWFGDINELTDQLDATLNQTVDAWFLDGF
CHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
APAKNPDMWTPNLFNAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKHKGFGRKREML
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHH
CGVMEQHLMPTLSAPWFYCSGSEKRETAIIGGGIASALLSLALLRRGWQVTLYCADDQPA
HHHHHHHCCCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
QGASGNRQGALYPLLSKHDAAINRFFPTAFTFARRLYDALPVSFDHAWCGVTQLGWDEKS
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHCCCCCHH
QQKIAQMLSLALPAGLASALNAEEAEQAVGVTTRCGGITYPAGGWLCPEQLTRAVIALAT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHH
EQGLQTRFRHTLTSLVAQESRWQLRFTSGETASHETVVLANGHQINRFDQTRPLPVYAVG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEECCCCCCCCCEEEEEC
GQVSHIPTTPALSALRQVLCYDGYLTPQNPHNQQHCIGASYHRGDESTVWREEDQRQNRQ
CEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RLLDCFPDANWATEVDVSGNSARCGVRCATRDHLPMVGNVPDYHATLTHYADLADNKTSA
HHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
ASAPVYPGLFMLGALGSRGLCSAPLCAEILAAQMSNEPIPLDAGTLAALNPNRLWVRKLL
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHH
KGKAVK
CCCCCC
>Mature Secondary Structure
MKQYAIQPATLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLAERFPVHSHPLF
CCCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHCCCCCCCEE
IVAESGFGTGLNFLTLWQAFDSFRSAHPQATLQRLHFISFEKFPLTRDDLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
APWAEQLQAQWPLPLPGCHRLLLDRGRVTLDLWFGDINELTDQLDATLNQTVDAWFLDGF
CHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
APAKNPDMWTPNLFNAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKHKGFGRKREML
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHH
CGVMEQHLMPTLSAPWFYCSGSEKRETAIIGGGIASALLSLALLRRGWQVTLYCADDQPA
HHHHHHHCCCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
QGASGNRQGALYPLLSKHDAAINRFFPTAFTFARRLYDALPVSFDHAWCGVTQLGWDEKS
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHCCCCCHH
QQKIAQMLSLALPAGLASALNAEEAEQAVGVTTRCGGITYPAGGWLCPEQLTRAVIALAT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHH
EQGLQTRFRHTLTSLVAQESRWQLRFTSGETASHETVVLANGHQINRFDQTRPLPVYAVG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEECCCCCCCCCEEEEEC
GQVSHIPTTPALSALRQVLCYDGYLTPQNPHNQQHCIGASYHRGDESTVWREEDQRQNRQ
CEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RLLDCFPDANWATEVDVSGNSARCGVRCATRDHLPMVGNVPDYHATLTHYADLADNKTSA
HHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
ASAPVYPGLFMLGALGSRGLCSAPLCAEILAAQMSNEPIPLDAGTLAALNPNRLWVRKLL
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHH
KGKAVK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA