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The map label for this gene is hemL [H]

Identifier: 161486604

GI number: 161486604

Start: 2769232

End: 2770527

Strand: Direct

Name: hemL [H]

Synonym: VV2728

Alternate gene names: 161486604

Gene position: 2769232-2770527 (Clockwise)

Preceding gene: 37680908

Following gene: 37680913

Centisome position: 82.55

GC content: 50.08

Gene sequence:

>1296_bases
ATGACCAAATCAGCCGAGCTGTATCAAAAAGCCCAGCAAACCATTCCTGGTGGCGTTAACTCACCCGTTCGTGCGTTCAA
CGGCGTTGGCGGTTCGCCAATTTTCGTCGAAAGAGCCGATGGCGCTTTCATTTTTGATGCGGATGGCAAAGCCTACATCG
ATTACGTAGGTTCATGGGGTCCGATGATTTTGGGCCATAACCACGCGGTGATTCGTGAAGCAGTGATTGAAGCTGCGCAA
CGTGGCCTCAGCTTTGGTGCTCCGACCGAAATGGAAATCAAAATGGCTGAATTGGTTTCCGAGCTCGTTCCTTCGATGGA
ACAGCTACGCATGGTCAGTTCAGGTACTGAAGCGACCATGAGTGCAATCCGCCTAGCGCGCGGCTTCACTGGCCGAGATA
AAATTCTAAAGTTTGAAGGTTGTTATCACGGCCACGCAGACAGCTTGCTTGTTAAAGCGGGCTCTGGTGCCTTGACTCTA
GGTCAACCAAGTTCTCCAGGTGTGCCTGCCGATTTCGCAAAACATACCCTAACCGCTACATTTAACGATCTCGATTCAGT
ACGAGAACTGTTTGCAGCAAACAAGGGTGAAATCGCCTGTATCATCGTTGAACCGGTGGCGGGCAACATGAACTGTATTC
CGCCAGTGGAAGGCTTCCACGAGGGGCTACGCGAGATCTGCGATCAAGAAGGCGCACTGCTGATTTTTGATGAAGTGATG
ACTGGCTTCCGTGTGGCACTTGGTGGTGCACAAGCTCACTACAACATCAAGCCAGACTTGACCACACTTGGTAAGGTCAT
TGGTGGTGGCATGCCAGTTGGTGCGTTTGGTGGCCGTAAAGAAGTCATGCAATATATTGCTCCAACCGGCCCTGTTTACC
AAGCTGGAACATTATCAGGTAACCCTGTAGCCATGGCGGCGGGCTACGCTTGTTTAACGTTGCTGCGTGAAGAAGGCAAT
GAAAAGCGTCTGGCATCAAAAACCAAACATCTGGCCGATGGCTTTAAATCTCTTGCAGCACAACATGGTATTCCACTTGT
GGTCAACCAAGTGGGCGGCATGTTTGGCTTCTTCTTTACTGAGCAAGAGCAGATCACTTGCTATGAAGATGTCACTAAGT
GCGATCTTGAACGCTTCAAACGCTTCTTCCACCTCATGATTGATCACGGTGTTTATTTGGCGCCTTCTGCCTTTGAAGCC
AGCTTCACCTCATTAGCTCACGGCAGCAAAGAGATTGAGGCGACATTGGAAGCGGCAGATCGTTGTTTTGCGATTTTGGC
AGCAGAAAGCAAGTAA

Upstream 100 bases:

>100_bases
CGTGAATAGAGTCATAAACCGAAGCAAAATATTTCTAACTGCCGTTGAGAGAGGTACAATGCCCGCCACTTGGTCCGAGC
AATCAGAAGAGGATAAACCC

Downstream 100 bases:

>100_bases
GATTGCCAACGCTCAACGAAAAAAATAAGAAAAGGATCGCAGTCAGCGATCCTTTTTCATTGCCAATTAAATATCACCAA
CAGCGCGAGCACGCCAATGG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTKSAELYQKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGAFIFDADGKAYIDYVGSWGPMILGHNHAVIREAVIEAAQ
RGLSFGAPTEMEIKMAELVSELVPSMEQLRMVSSGTEATMSAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKHTLTATFNDLDSVRELFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSGNPVAMAAGYACLTLLREEGN
EKRLASKTKHLADGFKSLAAQHGIPLVVNQVGGMFGFFFTEQEQITCYEDVTKCDLERFKRFFHLMIDHGVYLAPSAFEA
SFTSLAHGSKEIEATLEAADRCFAILAAESK

Sequences:

>Translated_431_residues
MTKSAELYQKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGAFIFDADGKAYIDYVGSWGPMILGHNHAVIREAVIEAAQ
RGLSFGAPTEMEIKMAELVSELVPSMEQLRMVSSGTEATMSAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKHTLTATFNDLDSVRELFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSGNPVAMAAGYACLTLLREEGN
EKRLASKTKHLADGFKSLAAQHGIPLVVNQVGGMFGFFFTEQEQITCYEDVTKCDLERFKRFFHLMIDHGVYLAPSAFEA
SFTSLAHGSKEIEATLEAADRCFAILAAESK
>Mature_430_residues
TKSAELYQKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGAFIFDADGKAYIDYVGSWGPMILGHNHAVIREAVIEAAQR
GLSFGAPTEMEIKMAELVSELVPSMEQLRMVSSGTEATMSAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTLG
QPSSPGVPADFAKHTLTATFNDLDSVRELFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVMT
GFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSGNPVAMAAGYACLTLLREEGNE
KRLASKTKHLADGFKSLAAQHGIPLVVNQVGGMFGFFFTEQEQITCYEDVTKCDLERFKRFFHLMIDHGVYLAPSAFEAS
FTSLAHGSKEIEATLEAADRCFAILAAESK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=27.9461279461279, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI37574042, Length=353, Percent_Identity=25.7790368271955, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226442705, Length=352, Percent_Identity=25.5681818181818, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI226442709, Length=318, Percent_Identity=25.7861635220126, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=71.830985915493, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=362, Percent_Identity=29.8342541436464, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788044, Length=313, Percent_Identity=31.9488817891374, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693181, Length=321, Percent_Identity=29.9065420560748, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787560, Length=348, Percent_Identity=30.1724137931034, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789759, Length=328, Percent_Identity=27.7439024390244, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786991, Length=390, Percent_Identity=27.1794871794872, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=28.2392026578073, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=359, Percent_Identity=26.4623955431755, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32564660, Length=357, Percent_Identity=26.6106442577031, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=144, Percent_Identity=34.0277777777778, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=382, Percent_Identity=28.5340314136126, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=350, Percent_Identity=24.2857142857143, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357415, Length=294, Percent_Identity=27.2108843537415, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46067; Mature: 45936

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSAELYQKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGAFIFDADGKAYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCC
PMILGHNHAVIREAVIEAAQRGLSFGAPTEMEIKMAELVSELVPSMEQLRMVSSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKHTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHH
FNDLDSVRELFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSG
HHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGYACLTLLREEGNEKRLASKTKHLADGFKSLAAQHGIPLVVNQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHEEEEC
EQEQITCYEDVTKCDLERFKRFFHLMIDHGVYLAPSAFEASFTSLAHGSKEIEATLEAAD
CCCCEEHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHH
RCFAILAAESK
HEEEEEEECCC
>Mature Secondary Structure 
TKSAELYQKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGAFIFDADGKAYIDYVGSWG
CCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCC
PMILGHNHAVIREAVIEAAQRGLSFGAPTEMEIKMAELVSELVPSMEQLRMVSSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKHTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHH
FNDLDSVRELFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSG
HHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGYACLTLLREEGNEKRLASKTKHLADGFKSLAAQHGIPLVVNQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHEEEEC
EQEQITCYEDVTKCDLERFKRFFHLMIDHGVYLAPSAFEASFTSLAHGSKEIEATLEAAD
CCCCEEHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHH
RCFAILAAESK
HEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA