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The map label for this gene is vacB

Identifier: 161486368

GI number: 161486368

Start: 4503632

End: 4506073

Strand: Direct

Name: vacB

Synonym: S4602

Alternate gene names: 161486368

Gene position: 4503632-4506073 (Clockwise)

Preceding gene: 30065550

Following gene: 30065552

Centisome position: 97.92

GC content: 55.12

Gene sequence:

>2442_bases
ATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACA
TTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAG
GCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAA
CGCCTCGACCTGGTGAAAGGTACCGTTATTGGTCACCGTGATGGCTACGGCTTTCTGCGGGTTGAAGGGCGTAAAGATGA
TTTGTATCTCTCCAGCGAGCAGATGAAAACCTGCATTCATGGCGATCAGGTGCTGGCGCAGCCGCTGGGTGCTGACCGTA
AAGGTCGTCGTGAAGCGCGTATTGTCCGCGTACTGGTGCCAAAAACCAGCCAGATTGTTGGTCGCTACTTTACCGAAGCG
GGCGTCGGCTTTGTGGTTCCTGACGATAGCCGTCTGAGCTTCGATATCTTAATCCCGCCCGATCAGATCATGGGCGCGCG
GATGGGCTTTGTGGTCGTAGTCGAACTGACTCAGCGTCCGACTCGCCGCACCAAAGCGGTGGGTAAAATCGTCGAAGTGC
TGGGCGACAATATGGGCACCGGCATGGCGGTTGATATCGCTCTGCGTACCCATGAAATTCCGTATATCTGGCCGCAGGCT
GTTGAGCAACAGGTTGCGGGGCTGAAAGAAGAAGTGCCGGAAGAAGCAAAAGCGGGCCGTGTCGATTTGCGCGATTTACC
GCTGGTCACCATTGATGGCGAAGACGCCCGTGACTTTGACGATGCAGTTTACTGCGAGAAAAAACGCGGCGGCGGCTGGC
GTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGCCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGC
ACGTCGGTGTACTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCA
GGTAGACCGCCTGTGTATGGTGTGCGAGATGACGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGG
TGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTAC
GCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAT
CTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACG
ACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCG
GCACTGTTCCGTATTCACGACAAGCCGAGCACCGAAGCGATTACCTCTTTCCGTTCAGTGCTGGCGGAGCTGGGGCTGGA
GCTGCCGGGTGGTAACAAGCCGGAACCGCGTGACTACGCGGAACTGCTGGAGTCGGTTGCCGACCGTCCTGATGCAGAAA
TGCTGCAAACCATGCTGCTACGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTCGGTCTGGCATTG
CAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCTGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGC
GAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGTTGCAACTGGGTC
AGCACTGTTCGATGGCGGAACGTCGTGCCGACGAAGCAACGCGCGATGTCGCTGACTGGCTGAAGTGTGACTTCATGCTC
GACCAGGTAGGTAACGTCTTTAAAGGCGTAATTTCCAGCGTCACTGGCTTTGGCTTCTTCGTCCGTCTGGACGACTTGTT
CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGG
AATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC
GACTTTAGTCTGATCTCCAGTGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGC
AGGCAAAAAAGGCGGCAAGCGTTGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAA
AAACGAAGCCGAAAGCGGCGAAGAAAGACGCGAGAAAAGCGAAAAAGCCATCGGCGAAAACGCAGAAAATAGCCGCAGCG
ACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGA

Upstream 100 bases:

>100_bases
ACACGCTTGCCGATTTGGTTGAAGAGAATCAACCGCTTTATAAATTATTGCTGGTGGAGTGACGAAAATCTTCATCAGAG
ATGACAACGGAGGAACCGAG

Downstream 100 bases:

>100_bases
TCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTT
ATCCGGCCTACATAATCACG

Product: exoribonuclease R

Products: NA

Alternate protein names: RNase R; Protein vacB [H]

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPE
RLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQA
VEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRG
TSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQY
APLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEP
ALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLAL
QSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFML
DQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKI
DFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAA
TKAKRAAKKKVAE

Sequences:

>Translated_813_residues
MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPE
RLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQA
VEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRG
TSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQY
APLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEP
ALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLAL
QSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFML
DQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKI
DFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAA
TKAKRAAKKKVAE
>Mature_812_residues
SQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPER
LDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEAG
VGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQAV
EQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGT
SVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQYA
PLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEPA
LFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQ
SYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFMLD
QVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKID
FSLISSERAPRNVGKTAREKAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAAT
KAKRAAKKKVAE

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs. Required for the expression of virulence genes in enteroinvasive strains of E.coli [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=556, Percent_Identity=28.9568345323741, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI190014623, Length=556, Percent_Identity=28.9568345323741, Blast_Score=186, Evalue=8e-47,
Organism=Homo sapiens, GI134288890, Length=416, Percent_Identity=30.2884615384615, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI19115966, Length=576, Percent_Identity=26.0416666666667, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI219521928, Length=575, Percent_Identity=25.9130434782609, Blast_Score=145, Evalue=2e-34,
Organism=Escherichia coli, GI87082383, Length=813, Percent_Identity=99.7539975399754, Blast_Score=1669, Evalue=0.0,
Organism=Escherichia coli, GI1787542, Length=651, Percent_Identity=26.1136712749616, Blast_Score=168, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI212645896, Length=419, Percent_Identity=31.2649164677804, Blast_Score=180, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17553506, Length=527, Percent_Identity=27.3244781783681, Blast_Score=166, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324552, Length=564, Percent_Identity=27.4822695035461, Blast_Score=158, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323943, Length=358, Percent_Identity=24.8603351955307, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649634, Length=480, Percent_Identity=29.5833333333333, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI19922976, Length=361, Percent_Identity=29.6398891966759, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24654597, Length=361, Percent_Identity=29.6398891966759, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24654592, Length=353, Percent_Identity=30.028328611898, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668 [H]

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 92067; Mature: 91936

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLR
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHH
AMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIH
HHHCCCCEEEEHHHHHHCHHHHHHHHCCEEECCCCCEEEEEECCCCCEEECHHHHHHHHC
GDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEAGVGFVVPDDSRLSFDILIPP
CHHHHHHCCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEECC
DQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQA
HHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHH
VEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIA
HHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCCCHHHEEECCCCCCEEEEEEEE
DVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVS
CCEEEECCCCCCCHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
SKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQYAPLVKHLEELHNLYKVLDKA
CCCCCCHHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEP
HHHHCCCCCCCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
ALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLL
CEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
RSMKQAIYDPENRGHFGLALQSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFF
CEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKI
EEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEECCCCHHH
DFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAA
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHH
KKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLR
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHH
AMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIH
HHHCCCCEEEEHHHHHHCHHHHHHHHCCEEECCCCCEEEEEECCCCCEEECHHHHHHHHC
GDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEAGVGFVVPDDSRLSFDILIPP
CHHHHHHCCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEECC
DQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQA
HHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHH
VEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIA
HHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCCCHHHEEECCCCCCEEEEEEEE
DVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVS
CCEEEECCCCCCCHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
SKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQYAPLVKHLEELHNLYKVLDKA
CCCCCCHHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEP
HHHHCCCCCCCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
ALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLL
CEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
RSMKQAIYDPENRGHFGLALQSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFF
CEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKI
EEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEECCCCHHH
DFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAA
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHH
KKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 3058695; 1400189; 9603904 [H]