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The map label for this gene is wecB [C]

Identifier: 160902742

GI number: 160902742

Start: 1377695

End: 1378768

Strand: Direct

Name: wecB [C]

Synonym: Pmob_1288

Alternate gene names: 160902742

Gene position: 1377695-1378768 (Clockwise)

Preceding gene: 160902741

Following gene: 160902743

Centisome position: 63.5

GC content: 32.68

Gene sequence:

>1074_bases
ATGAAACTACTCAGTTTAATAGGAGCTCGTCCACAGATAATCAAAGAAGCGATTCTAAATAAAGAATTTGAGAAAAAAGG
AATAAAAGAAATCTTGGTTCATTCTGGTCAGCATTACGACTTCAATATGTCAGATGTGTTCTTTAAAGTTTTAAACATAA
GAAAAGCTGATTACAATTTAGGGGTAGGTTCAGCAACGCATGCCCAAATGACGGCTAAAACAATGATAGAGTTTGAAAAG
GTTGTATTAAAAGAACATCCTGATATTATATTAGTGTATGGGGACACAAATACTACATTAGCGGGAGCAATAGTTGGAGC
AAAATTAAAGATCCCTGTTGCCCATGTGGAAGCAGGGATAAGGCAAGAACCAAAAGACATGCCTGAAGAGATAAATAGAG
TATTAACGGATCGTATATCAAAATACCTATTTTGTCCTTCTGAACTAGCTGTAAATAACTTAAAAAAAGAAGGTATTACT
GAAGGAGTGTATTTCACAGGAGATATAATGTACGATCTCTTTTTAAAGATGAAGCCATATTTCAAAGAAGATATTATAGA
CGAATTGAATTTGGAAGAAAATAAATACATAGTCACTACGATTCATAGGGATTTCAACACAGACAGTAAAGAAAAACTAG
AGAGTATATTGAAAGAGTTAGACAAAATAACAAAAGAAATAAAGGTTGTTTTTCCCATACATCCAAGAACCAAAAAGAGA
ATAGGTGAATTTAATTTTAATGAATACACAAAAAATATCTTATTGATAGAACCTCTAGATTACTTAAGTATGATGGGATT
GGTACAAAAGAGCCATCTTGTAATAACAGACAGTGGAGGATTGCAAAAAGAAGCATACTTTGCAGGGAAAAAAGCAATAG
TTGTTATGCCAGACACAGGTTGGAGAGAATTAACACAAGCAAGTTGGAATGTACTAAGTGAACCTAATGAAATCAAAAAT
AAGTTTCATCATATAATGAGCAACGAAATATCTTCAAATGTAGAAAATATATATGGAGACGGGAAAGCAGGGGAAAAGGT
TGTAACCTACCTAGAAAGTAATATTGTAAAGTAA

Upstream 100 bases:

>100_bases
ATAGATATTTATAAATTCTCAAGAGAATATCAAAACAACAAGCTAATTCCATTTATTAACAAATGAACTTCCCAAATTAT
AAAAGACGAGGAGAATTTAC

Downstream 100 bases:

>100_bases
AAAAATTGTGAAAAACGTAAGAAAGAGTTAGGAGGCTTTTAAATTTTCTATGATCTCTTTTAGCAAATCAAATCTGATTA
AAAATAAGAAGAAGATCGAC

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKLLSLIGARPQIIKEAILNKEFEKKGIKEILVHSGQHYDFNMSDVFFKVLNIRKADYNLGVGSATHAQMTAKTMIEFEK
VVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGIRQEPKDMPEEINRVLTDRISKYLFCPSELAVNNLKKEGIT
EGVYFTGDIMYDLFLKMKPYFKEDIIDELNLEENKYIVTTIHRDFNTDSKEKLESILKELDKITKEIKVVFPIHPRTKKR
IGEFNFNEYTKNILLIEPLDYLSMMGLVQKSHLVITDSGGLQKEAYFAGKKAIVVMPDTGWRELTQASWNVLSEPNEIKN
KFHHIMSNEISSNVENIYGDGKAGEKVVTYLESNIVK

Sequences:

>Translated_357_residues
MKLLSLIGARPQIIKEAILNKEFEKKGIKEILVHSGQHYDFNMSDVFFKVLNIRKADYNLGVGSATHAQMTAKTMIEFEK
VVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGIRQEPKDMPEEINRVLTDRISKYLFCPSELAVNNLKKEGIT
EGVYFTGDIMYDLFLKMKPYFKEDIIDELNLEENKYIVTTIHRDFNTDSKEKLESILKELDKITKEIKVVFPIHPRTKKR
IGEFNFNEYTKNILLIEPLDYLSMMGLVQKSHLVITDSGGLQKEAYFAGKKAIVVMPDTGWRELTQASWNVLSEPNEIKN
KFHHIMSNEISSNVENIYGDGKAGEKVVTYLESNIVK
>Mature_357_residues
MKLLSLIGARPQIIKEAILNKEFEKKGIKEILVHSGQHYDFNMSDVFFKVLNIRKADYNLGVGSATHAQMTAKTMIEFEK
VVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGIRQEPKDMPEEINRVLTDRISKYLFCPSELAVNNLKKEGIT
EGVYFTGDIMYDLFLKMKPYFKEDIIDELNLEENKYIVTTIHRDFNTDSKEKLESILKELDKITKEIKVVFPIHPRTKKR
IGEFNFNEYTKNILLIEPLDYLSMMGLVQKSHLVITDSGGLQKEAYFAGKKAIVVMPDTGWRELTQASWNVLSEPNEIKN
KFHHIMSNEISSNVENIYGDGKAGEKVVTYLESNIVK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=381, Percent_Identity=29.6587926509186, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 40701; Mature: 40701

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLSLIGARPQIIKEAILNKEFEKKGIKEILVHSGQHYDFNMSDVFFKVLNIRKADYNL
CCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
GVGSATHAQMTAKTMIEFEKVVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHEECCEEECCHHHHHHCC
RQEPKDMPEEINRVLTDRISKYLFCPSELAVNNLKKEGITEGVYFTGDIMYDLFLKMKPY
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCH
FKEDIIDELNLEENKYIVTTIHRDFNTDSKEKLESILKELDKITKEIKVVFPIHPRTKKR
HHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHH
IGEFNFNEYTKNILLIEPLDYLSMMGLVQKSHLVITDSGGLQKEAYFAGKKAIVVMPDTG
CCCCCCHHHHCCEEEECCHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCEEEEEECCCC
WRELTQASWNVLSEPNEIKNKFHHIMSNEISSNVENIYGDGKAGEKVVTYLESNIVK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLLSLIGARPQIIKEAILNKEFEKKGIKEILVHSGQHYDFNMSDVFFKVLNIRKADYNL
CCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
GVGSATHAQMTAKTMIEFEKVVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHEECCEEECCHHHHHHCC
RQEPKDMPEEINRVLTDRISKYLFCPSELAVNNLKKEGITEGVYFTGDIMYDLFLKMKPY
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCH
FKEDIIDELNLEENKYIVTTIHRDFNTDSKEKLESILKELDKITKEIKVVFPIHPRTKKR
HHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHH
IGEFNFNEYTKNILLIEPLDYLSMMGLVQKSHLVITDSGGLQKEAYFAGKKAIVVMPDTG
CCCCCCHHHHCCEEEECCHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCEEEEEECCCC
WRELTQASWNVLSEPNEIKNKFHHIMSNEISSNVENIYGDGKAGEKVVTYLESNIVK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA