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The map label for this gene is cls [H]

Identifier: 160902558

GI number: 160902558

Start: 1173437

End: 1174897

Strand: Direct

Name: cls [H]

Synonym: Pmob_1097

Alternate gene names: 160902558

Gene position: 1173437-1174897 (Clockwise)

Preceding gene: 160902557

Following gene: 160902559

Centisome position: 54.09

GC content: 30.87

Gene sequence:

>1461_bases
ATGGCCTTTGCTATAATTTTCAAATTTGGATTTTGGACTTTTTTTGTATTAATATATGCATTTGTTGCTACGACTGTTAT
CATCTTGGAAAGAAAAAGACCAGAAAAAACCATAAGTTGGCTTTTAATATTTGCCGTAATCCCACTTATAGGATTTGTAA
TCTATCTTTTTTTTGGAAGAAACTGGAAAAGAAGAAAACTTACCAGTGATATTTACCTGGATTCAAAACCCAGTTTATCA
GAAGAAGAAGAAAAATGGCTAATTAAGGTACTTGGGGAAAATGATACTCAATATTTACAACTAATTAATCTTTTAAAAAG
GAACTCTAAGTCGCCTTTATTTTTAGGAAATGACATAGAAATTTTTAATAATGGGAAAGAAAAATTTTCTTCTTTTTTTA
AAGAAATTGAAAATGCAAAAGAATCTATCCTTTTAGAATACTATATTGTAAAAGACGATGAAACCGGTAATAAACTCAAA
GATCTTCTGATAAAAAAGGCTAAAGAGGGCGTGGAAATTAAATTTATTATGGATAAAATAGGTTCAGGGCGTCTAAAGAA
AAGTTATATAAAGCAATTAAAAAGTGCAGGAGTTGAAATTGCTTTTTACAGCTATTTTCTATCGCCGGTGCTTAAATTCC
TAAATACTCAGGTTAATTACAGAAATCACAGAAAAATAGCGATAATTGACTCTGAAATCGGCTTTATTGGTGGAATAAAC
ATTGGAAATGAATACATTGGTAAAAGTAGTTTGGGATATTGGAGAGACCTTCACTTAAAAGTCAGGGGAGAGGCTGTAAA
CGGACTTCAAAAAATTTTCTTTGAAGACTATCAAACAATTGTAAAGGCAGAGAGAAAAAAAGAAAAAATAGATCCTAGTA
AATACTTTAAAAAACAAGAGAGAAAAGGAGACTCATTAATACAAATAGCGGTGAGTGGCCCAGAATCAGAAAATTCATCG
ATAATGCAAACCATCCATAAGATGATTACTATTGCTAAAGACCATATATACATAGCTACCCCGTACTTCATTCCAGATGA
TAGCATTCTTTCTGCCTTGAAAATAGCGGCCCTTTCCGGTGTTAAAGTAAAAATTTTATTTCCTGGAAAGATGGATCATT
TTTTAGTATATTTTGCTTCAAGGACTTATTTAGAAGAAATTATAAAATATGGTGTTGAAGTGTACTTTTACAGAAAAGAC
CGGTTCATACACTCCAAATTTGTATCGATAGATGGCTTAATATCAACCGTTGGAACCGCAAATATAGATATCAGAAGCTT
CGAACTAAATTATGAGGCAAACCTTTTAATATACGATAGAGAAAAAACTAAACAAATAGAAGATATTTTTCATGAGGATT
TAAAAATTAGTTCCCGCATATCTCAAGATTACTTTAATGCTCAGCCTTTCCTTATCAAATTTACAGAAGCATTCTGTAAG
ATTTTCTCTAATCTTTTGTAA

Upstream 100 bases:

>100_bases
AACTGTTTAAAAGAAAACGAAGAATTAAAATCAATCGAAGAAATTCACGAAGAGATCTACTCTATATGGAAACAAATAAG
AGATTTAGGAGAAATATAAA

Downstream 100 bases:

>100_bases
AAAGTGCAATTTTTACTTGACAACCTTTATTTGTTGGTGTATAATCATAAAAATTAACTTTCAATTAAGCGAGGGTTACT
ATGAAAAAAATTTCATTGCA

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAFAIIFKFGFWTFFVLIYAFVATTVIILERKRPEKTISWLLIFAVIPLIGFVIYLFFGRNWKRRKLTSDIYLDSKPSLS
EEEEKWLIKVLGENDTQYLQLINLLKRNSKSPLFLGNDIEIFNNGKEKFSSFFKEIENAKESILLEYYIVKDDETGNKLK
DLLIKKAKEGVEIKFIMDKIGSGRLKKSYIKQLKSAGVEIAFYSYFLSPVLKFLNTQVNYRNHRKIAIIDSEIGFIGGIN
IGNEYIGKSSLGYWRDLHLKVRGEAVNGLQKIFFEDYQTIVKAERKKEKIDPSKYFKKQERKGDSLIQIAVSGPESENSS
IMQTIHKMITIAKDHIYIATPYFIPDDSILSALKIAALSGVKVKILFPGKMDHFLVYFASRTYLEEIIKYGVEVYFYRKD
RFIHSKFVSIDGLISTVGTANIDIRSFELNYEANLLIYDREKTKQIEDIFHEDLKISSRISQDYFNAQPFLIKFTEAFCK
IFSNLL

Sequences:

>Translated_486_residues
MAFAIIFKFGFWTFFVLIYAFVATTVIILERKRPEKTISWLLIFAVIPLIGFVIYLFFGRNWKRRKLTSDIYLDSKPSLS
EEEEKWLIKVLGENDTQYLQLINLLKRNSKSPLFLGNDIEIFNNGKEKFSSFFKEIENAKESILLEYYIVKDDETGNKLK
DLLIKKAKEGVEIKFIMDKIGSGRLKKSYIKQLKSAGVEIAFYSYFLSPVLKFLNTQVNYRNHRKIAIIDSEIGFIGGIN
IGNEYIGKSSLGYWRDLHLKVRGEAVNGLQKIFFEDYQTIVKAERKKEKIDPSKYFKKQERKGDSLIQIAVSGPESENSS
IMQTIHKMITIAKDHIYIATPYFIPDDSILSALKIAALSGVKVKILFPGKMDHFLVYFASRTYLEEIIKYGVEVYFYRKD
RFIHSKFVSIDGLISTVGTANIDIRSFELNYEANLLIYDREKTKQIEDIFHEDLKISSRISQDYFNAQPFLIKFTEAFCK
IFSNLL
>Mature_485_residues
AFAIIFKFGFWTFFVLIYAFVATTVIILERKRPEKTISWLLIFAVIPLIGFVIYLFFGRNWKRRKLTSDIYLDSKPSLSE
EEEKWLIKVLGENDTQYLQLINLLKRNSKSPLFLGNDIEIFNNGKEKFSSFFKEIENAKESILLEYYIVKDDETGNKLKD
LLIKKAKEGVEIKFIMDKIGSGRLKKSYIKQLKSAGVEIAFYSYFLSPVLKFLNTQVNYRNHRKIAIIDSEIGFIGGINI
GNEYIGKSSLGYWRDLHLKVRGEAVNGLQKIFFEDYQTIVKAERKKEKIDPSKYFKKQERKGDSLIQIAVSGPESENSSI
MQTIHKMITIAKDHIYIATPYFIPDDSILSALKIAALSGVKVKILFPGKMDHFLVYFASRTYLEEIIKYGVEVYFYRKDR
FIHSKFVSIDGLISTVGTANIDIRSFELNYEANLLIYDREKTKQIEDIFHEDLKISSRISQDYFNAQPFLIKFTEAFCKI
FSNLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=490, Percent_Identity=25.1020408163265, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787007, Length=335, Percent_Identity=31.6417910447761, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 56482; Mature: 56351

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFAIIFKFGFWTFFVLIYAFVATTVIILERKRPEKTISWLLIFAVIPLIGFVIYLFFGR
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NWKRRKLTSDIYLDSKPSLSEEEEKWLIKVLGENDTQYLQLINLLKRNSKSPLFLGNDIE
CCCHHCCCCCEEECCCCCCCCHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCEEECCCEE
IFNNGKEKFSSFFKEIENAKESILLEYYIVKDDETGNKLKDLLIKKAKEGVEIKFIMDKI
EECCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHC
GSGRLKKSYIKQLKSAGVEIAFYSYFLSPVLKFLNTQVNYRNHRKIAIIDSEIGFIGGIN
CCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCC
IGNEYIGKSSLGYWRDLHLKVRGEAVNGLQKIFFEDYQTIVKAERKKEKIDPSKYFKKQE
CCCHHCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RKGDSLIQIAVSGPESENSSIMQTIHKMITIAKDHIYIATPYFIPDDSILSALKIAALSG
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECEECCCHHHHHHHHHHHHCC
VKVKILFPGKMDHFLVYFASRTYLEEIIKYGVEVYFYRKDRFIHSKFVSIDGLISTVGTA
CEEEEEECCCCCHHHEEHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
NIDIRSFELNYEANLLIYDREKTKQIEDIFHEDLKISSRISQDYFNAQPFLIKFTEAFCK
CEEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
IFSNLL
HHHHCC
>Mature Secondary Structure 
AFAIIFKFGFWTFFVLIYAFVATTVIILERKRPEKTISWLLIFAVIPLIGFVIYLFFGR
EEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NWKRRKLTSDIYLDSKPSLSEEEEKWLIKVLGENDTQYLQLINLLKRNSKSPLFLGNDIE
CCCHHCCCCCEEECCCCCCCCHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCEEECCCEE
IFNNGKEKFSSFFKEIENAKESILLEYYIVKDDETGNKLKDLLIKKAKEGVEIKFIMDKI
EECCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHC
GSGRLKKSYIKQLKSAGVEIAFYSYFLSPVLKFLNTQVNYRNHRKIAIIDSEIGFIGGIN
CCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCC
IGNEYIGKSSLGYWRDLHLKVRGEAVNGLQKIFFEDYQTIVKAERKKEKIDPSKYFKKQE
CCCHHCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RKGDSLIQIAVSGPESENSSIMQTIHKMITIAKDHIYIATPYFIPDDSILSALKIAALSG
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECEECCCHHHHHHHHHHHHCC
VKVKILFPGKMDHFLVYFASRTYLEEIIKYGVEVYFYRKDRFIHSKFVSIDGLISTVGTA
CEEEEEECCCCCHHHEEHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
NIDIRSFELNYEANLLIYDREKTKQIEDIFHEDLKISSRISQDYFNAQPFLIKFTEAFCK
CEEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
IFSNLL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA