Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 160902482

GI number: 160902482

Start: 1086358

End: 1086948

Strand: Direct

Name: hisH [H]

Synonym: Pmob_1019

Alternate gene names: 160902482

Gene position: 1086358-1086948 (Clockwise)

Preceding gene: 160902481

Following gene: 160902483

Centisome position: 50.07

GC content: 31.3

Gene sequence:

>591_bases
ATGCAGAAAATAGTCATATTAAATGGTGGAGTTGGTAACTTTTCAAATGTCCAAAAAGCTGTCGGAGGTATTATTAGCAA
TGAAATAAATGACATAAAAAAAGCAGATAAAATCATACTTCCAGGGGTAGGCTCTTTTGGAATAGTTTCAAATAATATAA
TTCCTCTAAAAGATTATATATTGGAACATATCGACAAAAACAAGCCTTTCTTAGGTATATGTTTAGGGATGCAACTGTTA
TTTGAAAGTAGCGAAGAAGGTGAAGGCACAGGACTTTCTTATTTACCTGGAAAAGTGGTTAAGTTTAAAAACATGAAAGT
ACCTCACATAGGTTGGAACAGTGTTGAATTTTCAAAAGATTCTCTGATTTTTCGAGGAATAGAAAATGGAAGTTTCTTCT
ATTTTGTACACTCTTATTACATAACAACTGAAGATCAATACACAATTTCATACACAGAATATGAAAGCAGAGGAAAAACC
TGTAAATTCACATCAAGCGTGCAAAAAGAAAACGTATTCGGAGTACAGTTCCATCCTGAAAAATCAGGCGAAAAAGGAAT
TAAATTATTAGAAAATTTCAAAAATTTATAA

Upstream 100 bases:

>100_bases
CTTCTTTCAAGGCATTGGGTAGCGCTTTAAAAACGGCCCTTGAACCAAGTGACAAACTCGAAAGTACAAATAGGGTTTAC
GAAGTATAGAGGTGGTAGAT

Downstream 100 bases:

>100_bases
CGAGGTGAAATCTATCAACAACATAAGAATAATACCTGCAATAGACTTAATGAATAAAAAAGCGGTAAGACTCTATAAAG
GGAGTAAAAACGAAATAAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MQKIVILNGGVGNFSNVQKAVGGIISNEINDIKKADKIILPGVGSFGIVSNNIIPLKDYILEHIDKNKPFLGICLGMQLL
FESSEEGEGTGLSYLPGKVVKFKNMKVPHIGWNSVEFSKDSLIFRGIENGSFFYFVHSYYITTEDQYTISYTEYESRGKT
CKFTSSVQKENVFGVQFHPEKSGEKGIKLLENFKNL

Sequences:

>Translated_196_residues
MQKIVILNGGVGNFSNVQKAVGGIISNEINDIKKADKIILPGVGSFGIVSNNIIPLKDYILEHIDKNKPFLGICLGMQLL
FESSEEGEGTGLSYLPGKVVKFKNMKVPHIGWNSVEFSKDSLIFRGIENGSFFYFVHSYYITTEDQYTISYTEYESRGKT
CKFTSSVQKENVFGVQFHPEKSGEKGIKLLENFKNL
>Mature_196_residues
MQKIVILNGGVGNFSNVQKAVGGIISNEINDIKKADKIILPGVGSFGIVSNNIIPLKDYILEHIDKNKPFLGICLGMQLL
FESSEEGEGTGLSYLPGKVVKFKNMKVPHIGWNSVEFSKDSLIFRGIENGSFFYFVHSYYITTEDQYTISYTEYESRGKT
CKFTSSVQKENVFGVQFHPEKSGEKGIKLLENFKNL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=39, Blast_Score=131, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=177, Percent_Identity=37.2881355932203, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 21934; Mature: 21934

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIVILNGGVGNFSNVQKAVGGIISNEINDIKKADKIILPGVGSFGIVSNNIIPLKDYI
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEECCCEECHHHHH
LEHIDKNKPFLGICLGMQLLFESSEEGEGTGLSYLPGKVVKFKNMKVPHIGWNSVEFSKD
HHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCEECCC
SLIFRGIENGSFFYFVHSYYITTEDQYTISYTEYESRGKTCKFTSSVQKENVFGVQFHPE
CEEEEECCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEECCCCCCCCEEEEEECCC
KSGEKGIKLLENFKNL
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQKIVILNGGVGNFSNVQKAVGGIISNEINDIKKADKIILPGVGSFGIVSNNIIPLKDYI
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEECCCEECHHHHH
LEHIDKNKPFLGICLGMQLLFESSEEGEGTGLSYLPGKVVKFKNMKVPHIGWNSVEFSKD
HHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCCEECCC
SLIFRGIENGSFFYFVHSYYITTEDQYTISYTEYESRGKTCKFTSSVQKENVFGVQFHPE
CEEEEECCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEECCCCCCCCEEEEEECCC
KSGEKGIKLLENFKNL
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA