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The map label for this gene is pfkA1 [H]

Identifier: 160902457

GI number: 160902457

Start: 1058159

End: 1059175

Strand: Direct

Name: pfkA1 [H]

Synonym: Pmob_0993

Alternate gene names: 160902457

Gene position: 1058159-1059175 (Clockwise)

Preceding gene: 160902456

Following gene: 160902475

Centisome position: 48.77

GC content: 37.46

Gene sequence:

>1017_bases
ATGAAAAGAGTCGCTGTTTTAAATGTAGGTGGAGATTGTCCAGGATTAAATGCCGTAATCCGGGCACTCATCGTTAAAGG
CGCCGAAGAGGATATCGAAGTTGTCGGCGTTTACGACGGTTTTCTAGGTCTCGTGGAAGATAAATTAACAATCTTAGCTA
AAGAACATGTCTCAGGTAAATTACCTGAAGGTGGCATAATTTTGGGTTCGTCAAAATACGATCCTACTGTTAATCCAAAC
GATTTGAAAAAGTTGAAAAATAACTTTGAGAGATATCAGATAACTAGTCTTATATTGTTAACAGGCCATACCGGAGCAAA
TATCGCCTTAAAATTGGCAAATGAAGGTATACCTTCGATAATAATACCTGCTACCGTTGATAATGACTTATACTGGACGG
ATCTTAGCGTTGGTTTTCTAACGGCATTACAGATCGTCACCGATGCATTAGATAAACTTCATTCAACTGCGAGTGCAGGT
CATAGGGTAATAGTTGTAGAAACCGGAGGAGACGAAGCAGGATGGTTAGCAACAATTGGTGGTATGGCAGGTGGTGCTGA
TTATATAATAGTACCAGAGTTCAAGTTAGACCCTAAAGATATGATTGAAAATATTAAAAGGAGATATTCTGCAGGTAGAA
GATTTTCCATAGTTGTAGTAGAAGAAAAAGTCAAACTCCCCGAAGAAGTTCAAAATATAATAGGCGATCCAAAGGTTCGT
CAATACATGAAACCAGCAGAATTAATCACAGAGTACATAAAAGCTAATCTGCAAAATGTTGAATGTAGAACCGTTGATTT
AGATTATTTACAAAGAGGAGGAACACCTTCGTCTTTTGATAGATACCTGGCTTTCAAATTCGGTGTTTCGGCTATAGACG
CTGTTAAAAAAGGAAAATCTAATGTGGCTTTAGGTTTGGATGGTTTTGATGTTGTGGAAAAACCATTTACCCACGAAATT
TTGAAGAACAAAGAGATAAGTAGAGAATTATACGAAATGGGTAGACTTTTCTTCTAA

Upstream 100 bases:

>100_bases
TTTTTCCTTATGGGTGGGGAGCGGGGCGAAGGGGCGCTAACACAAGGTTTTAGAGTTCCTAAAGTTAGTATAATCAAATT
AAAATGTGGAGGTGTATATA

Downstream 100 bases:

>100_bases
AAATATTTAGCGGTGCTTTTTGCACCGCTATTTTTAAACGCATTATTAAAATCCACAAATAATAAACTTGCTTGACTTTT
TGATCATATTTTATAAAGTT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKRVAVLNVGGDCPGLNAVIRALIVKGAEEDIEVVGVYDGFLGLVEDKLTILAKEHVSGKLPEGGIILGSSKYDPTVNPN
DLKKLKNNFERYQITSLILLTGHTGANIALKLANEGIPSIIIPATVDNDLYWTDLSVGFLTALQIVTDALDKLHSTASAG
HRVIVVETGGDEAGWLATIGGMAGGADYIIVPEFKLDPKDMIENIKRRYSAGRRFSIVVVEEKVKLPEEVQNIIGDPKVR
QYMKPAELITEYIKANLQNVECRTVDLDYLQRGGTPSSFDRYLAFKFGVSAIDAVKKGKSNVALGLDGFDVVEKPFTHEI
LKNKEISRELYEMGRLFF

Sequences:

>Translated_338_residues
MKRVAVLNVGGDCPGLNAVIRALIVKGAEEDIEVVGVYDGFLGLVEDKLTILAKEHVSGKLPEGGIILGSSKYDPTVNPN
DLKKLKNNFERYQITSLILLTGHTGANIALKLANEGIPSIIIPATVDNDLYWTDLSVGFLTALQIVTDALDKLHSTASAG
HRVIVVETGGDEAGWLATIGGMAGGADYIIVPEFKLDPKDMIENIKRRYSAGRRFSIVVVEEKVKLPEEVQNIIGDPKVR
QYMKPAELITEYIKANLQNVECRTVDLDYLQRGGTPSSFDRYLAFKFGVSAIDAVKKGKSNVALGLDGFDVVEKPFTHEI
LKNKEISRELYEMGRLFF
>Mature_338_residues
MKRVAVLNVGGDCPGLNAVIRALIVKGAEEDIEVVGVYDGFLGLVEDKLTILAKEHVSGKLPEGGIILGSSKYDPTVNPN
DLKKLKNNFERYQITSLILLTGHTGANIALKLANEGIPSIIIPATVDNDLYWTDLSVGFLTALQIVTDALDKLHSTASAG
HRVIVVETGGDEAGWLATIGGMAGGADYIIVPEFKLDPKDMIENIKRRYSAGRRFSIVVVEEKVKLPEEVQNIIGDPKVR
QYMKPAELITEYIKANLQNVECRTVDLDYLQRGGTPSSFDRYLAFKFGVSAIDAVKKGKSNVALGLDGFDVVEKPFTHEI
LKNKEISRELYEMGRLFF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=362, Percent_Identity=28.4530386740331, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI11321601, Length=364, Percent_Identity=27.7472527472527, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI266453619, Length=365, Percent_Identity=27.9452054794521, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI266453768, Length=365, Percent_Identity=27.9452054794521, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI266453748, Length=365, Percent_Identity=27.9452054794521, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4505749, Length=365, Percent_Identity=27.9452054794521, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1790350, Length=342, Percent_Identity=31.2865497076023, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17558788, Length=323, Percent_Identity=30.030959752322, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25147584, Length=361, Percent_Identity=25.207756232687, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI72003601, Length=345, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321679, Length=323, Percent_Identity=30.030959752322, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323861, Length=368, Percent_Identity=26.3586956521739, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652337, Length=362, Percent_Identity=29.0055248618785, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17647809, Length=362, Percent_Identity=29.0055248618785, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28573326, Length=362, Percent_Identity=28.1767955801105, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37050; Mature: 37050

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVAVLNVGGDCPGLNAVIRALIVKGAEEDIEVVGVYDGFLGLVEDKLTILAKEHVSGK
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
LPEGGIILGSSKYDPTVNPNDLKKLKNNFERYQITSLILLTGHTGANIALKLANEGIPSI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCCCEEEEEECCCCCEE
IIPATVDNDLYWTDLSVGFLTALQIVTDALDKLHSTASAGHRVIVVETGGDEAGWLATIG
EEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEEEC
GMAGGADYIIVPEFKLDPKDMIENIKRRYSAGRRFSIVVVEEKVKLPEEVQNIIGDPKVR
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCHHHH
QYMKPAELITEYIKANLQNVECRTVDLDYLQRGGTPSSFDRYLAFKFGVSAIDAVKKGKS
HHCCHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHCCCC
NVALGLDGFDVVEKPFTHEILKNKEISRELYEMGRLFF
CEEEECCCHHHHCCCHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRVAVLNVGGDCPGLNAVIRALIVKGAEEDIEVVGVYDGFLGLVEDKLTILAKEHVSGK
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
LPEGGIILGSSKYDPTVNPNDLKKLKNNFERYQITSLILLTGHTGANIALKLANEGIPSI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCCCEEEEEECCCCCEE
IIPATVDNDLYWTDLSVGFLTALQIVTDALDKLHSTASAGHRVIVVETGGDEAGWLATIG
EEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEEEC
GMAGGADYIIVPEFKLDPKDMIENIKRRYSAGRRFSIVVVEEKVKLPEEVQNIIGDPKVR
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCHHHH
QYMKPAELITEYIKANLQNVECRTVDLDYLQRGGTPSSFDRYLAFKFGVSAIDAVKKGKS
HHCCHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHCCCC
NVALGLDGFDVVEKPFTHEILKNKEISRELYEMGRLFF
CEEEECCCHHHHCCCHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]