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The map label for this gene is wecB [C]

Identifier: 160902398

GI number: 160902398

Start: 987928

End: 988989

Strand: Direct

Name: wecB [C]

Synonym: Pmob_0933

Alternate gene names: 160902398

Gene position: 987928-988989 (Clockwise)

Preceding gene: 160902397

Following gene: 160902399

Centisome position: 45.54

GC content: 32.2

Gene sequence:

>1062_bases
ATGAAACTAATCAGTTTGATAGGAGCTCGTCCGCAGATAATCAAAGAAGCGATTATAAATAAAGAATTTGAGAAAAAAGG
AATAAAAGAAATCTTGGTTCATTCTGGCCAACATTACGACTTCAATATGTCAGATGTGTTCTTTCAAGTTTTAAACATAA
GAAAAGCCGATTACAATTTAGGAGTAGGTTCAGCAACGCATGCTCAAATGACGGCTAAAACCATGATAGAGTTTGAAAAG
GTTGTATTAAAAGAACATCCTGATATTATATTAGTATATGGAGACACAAATACTACATTAGCTGGAGCAATAGTAGGAGC
AAAATTAAAGATCCCTGTTGCACACGTGGAAGCTGGAATAAGGCAAGAACCAAAGGACATGCCTGAAGAAATAAATAGAG
TATTAACAGATAGAATATCAAAATACCTATTTTGTCCTTCTGAAGTAGCTGTAAACAACTTAAAAAAGGAAAGTATCACT
GAAGGAGTGCATTTCACAGGAGATATAATGTACGATCTTTTTTTAAAGATGAGACCATATTTCAAAGAAGATATTATAGA
CGAATTGAGTTTGGAAGAAAATAAATACATAGTGACTACAATTCATAGAGATTTCAATACAGATAGTAAAGAAAAACTAG
AGAATATATTAAAAGAGTTAGATAAAATATCAAAAGAAATAAAGGTTGTCTTTCCCATACATCCAAGAACCAAAAAGAAA
ATAGATGAATTCAATCTTAACAAATACACAAAAGATATTCTACTGATAGAACCTCTTGATTACTTAAGTATGATGGGATT
GGTACAAAAAAGCCTTCTTGTAATAACAGACAGTGGAGGGTTGCAAAAAGAAGCATACTTTGCAAGGAAAAGAGCGATAG
TTGTTATGCCAGACACAGGCTGGAGAGAGTTAACACAAGCAGATTGGAATATACTAAGTGAACCTGATGAAATCAAAAAT
AAGATGGATTATATAATGAACAACGAAATATCTTCAAATGTAGAAAACATATATGGAGACGGGAAACCAGGGGAAAAGAT
AGTAAAATTATTAAGCATTTGA

Upstream 100 bases:

>100_bases
TATATTGACTTGGGAAGAAAGAATGAAAATAGAAATAAATAAAATAGATGAAATTATTGCAAAGTGGGACAAATAATTTC
ACAAAAACGAGGGAAATATC

Downstream 100 bases:

>100_bases
CAAATACCAAAATAGTTACTATTATCTTTATATATTGTTCAAAAATTGAACGAAGGTGTTGAATATTATGCAAGATAATG
AGATACTCATTCTAGAAGAA

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKLISLIGARPQIIKEAIINKEFEKKGIKEILVHSGQHYDFNMSDVFFQVLNIRKADYNLGVGSATHAQMTAKTMIEFEK
VVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGIRQEPKDMPEEINRVLTDRISKYLFCPSEVAVNNLKKESIT
EGVHFTGDIMYDLFLKMRPYFKEDIIDELSLEENKYIVTTIHRDFNTDSKEKLENILKELDKISKEIKVVFPIHPRTKKK
IDEFNLNKYTKDILLIEPLDYLSMMGLVQKSLLVITDSGGLQKEAYFARKRAIVVMPDTGWRELTQADWNILSEPDEIKN
KMDYIMNNEISSNVENIYGDGKPGEKIVKLLSI

Sequences:

>Translated_353_residues
MKLISLIGARPQIIKEAIINKEFEKKGIKEILVHSGQHYDFNMSDVFFQVLNIRKADYNLGVGSATHAQMTAKTMIEFEK
VVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGIRQEPKDMPEEINRVLTDRISKYLFCPSEVAVNNLKKESIT
EGVHFTGDIMYDLFLKMRPYFKEDIIDELSLEENKYIVTTIHRDFNTDSKEKLENILKELDKISKEIKVVFPIHPRTKKK
IDEFNLNKYTKDILLIEPLDYLSMMGLVQKSLLVITDSGGLQKEAYFARKRAIVVMPDTGWRELTQADWNILSEPDEIKN
KMDYIMNNEISSNVENIYGDGKPGEKIVKLLSI
>Mature_353_residues
MKLISLIGARPQIIKEAIINKEFEKKGIKEILVHSGQHYDFNMSDVFFQVLNIRKADYNLGVGSATHAQMTAKTMIEFEK
VVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGIRQEPKDMPEEINRVLTDRISKYLFCPSEVAVNNLKKESIT
EGVHFTGDIMYDLFLKMRPYFKEDIIDELSLEENKYIVTTIHRDFNTDSKEKLENILKELDKISKEIKVVFPIHPRTKKK
IDEFNLNKYTKDILLIEPLDYLSMMGLVQKSLLVITDSGGLQKEAYFARKRAIVVMPDTGWRELTQADWNILSEPDEIKN
KMDYIMNNEISSNVENIYGDGKPGEKIVKLLSI

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=375, Percent_Identity=28, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 40409; Mature: 40409

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISLIGARPQIIKEAIINKEFEKKGIKEILVHSGQHYDFNMSDVFFQVLNIRKADYNL
CCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
GVGSATHAQMTAKTMIEFEKVVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHEECCEEECCHHHHHHCC
RQEPKDMPEEINRVLTDRISKYLFCPSEVAVNNLKKESITEGVHFTGDIMYDLFLKMRPY
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
FKEDIIDELSLEENKYIVTTIHRDFNTDSKEKLENILKELDKISKEIKVVFPIHPRTKKK
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHH
IDEFNLNKYTKDILLIEPLDYLSMMGLVQKSLLVITDSGGLQKEAYFARKRAIVVMPDTG
HHHCCCCHHHCCEEEECCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHCCEEEEECCCC
WRELTQADWNILSEPDEIKNKMDYIMNNEISSNVENIYGDGKPGEKIVKLLSI
HHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKLISLIGARPQIIKEAIINKEFEKKGIKEILVHSGQHYDFNMSDVFFQVLNIRKADYNL
CCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
GVGSATHAQMTAKTMIEFEKVVLKEHPDIILVYGDTNTTLAGAIVGAKLKIPVAHVEAGI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHEECCEEECCHHHHHHCC
RQEPKDMPEEINRVLTDRISKYLFCPSEVAVNNLKKESITEGVHFTGDIMYDLFLKMRPY
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
FKEDIIDELSLEENKYIVTTIHRDFNTDSKEKLENILKELDKISKEIKVVFPIHPRTKKK
HHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHH
IDEFNLNKYTKDILLIEPLDYLSMMGLVQKSLLVITDSGGLQKEAYFARKRAIVVMPDTG
HHHCCCCHHHCCEEEECCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHCCEEEEECCCC
WRELTQADWNILSEPDEIKNKMDYIMNNEISSNVENIYGDGKPGEKIVKLLSI
HHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA