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The map label for this gene is tilS

Identifier: 160902337

GI number: 160902337

Start: 926219

End: 927154

Strand: Direct

Name: tilS

Synonym: Pmob_0872

Alternate gene names: 160902337

Gene position: 926219-927154 (Clockwise)

Preceding gene: 160902333

Following gene: 160902338

Centisome position: 42.69

GC content: 29.27

Gene sequence:

>936_bases
GTGATACTCCAAGATTTTGAGAAAAAAATATTTAAATTTATAAGAGACTATAAAATTTTTAACAAATATGATAAAATTCT
ATTAGGAGTTTCCGGTGGAAAAGATTCAATGTCTTTGCTTCATGTGATGTCAAAATTATCGAAAACTATGGGATTTGAAA
TATCTGTAGCCCATTTAAATCATTGTATGAGAGAAGAAGCTGATAATGACGAAGCTTTTGTGAGACAAGCTTGTTGGAAA
CTAAAGATACCCTTTTTTTCGAAAAAAGTGGATGTTTTTACATACTCAAAAAAAAACAAGGTAGGCGTTGAAGTAGCGGG
AAGAAAATTAAGATATGAATTTTTTTATGAAACATTAAGAAGATTAAGTTATAATAAAATAGCAACCGCCCATCATATGG
ATGATTTATTTGAAACAATGATTTACAGAATTTTGAGAGGGACCGGAATATATGGATTAGGCGGTCTAATTCCTATAGAA
GAAGAAATAACAAAACCAATGTTATGTGTTGACTTGGAAGAAATCAAAAATTATGTTACAATTAATAATATAGAGTATGT
GGAAGATAAGTACAATTATTCTTTAGATTATGCTAGAAATAAAATAAGATATGAAATCACTCCTTTATTTAAGAAGATCA
ACCCAAGGTACAAGGAAAGTTTCTTCCGACTTGCAAAAATCATTTGGAGTTACAGGGAAGAAGTTAAAAGGAAATTTGAA
GAAAGAAGTGAGATCAGTAAAGACTCATTAAAATTAAGATTAGAAAATGACTTTTTCGATGGCGAAATAATAAGAATTGC
CTTTTTGAAGTTTGGTAAATATCCTCCAAATATGGAGGAAACAGAGAAAATCATAAAAATGCGCAAGGGTGGAGTAAGAA
AGATAAATGGGTTAAGTATAACAAAAAAAAGTGATTCTCTGCTAGTTAAAATTTAA

Upstream 100 bases:

>100_bases
CTTCACCGGCTCCACCATATTTTTAGTAGTCAAAGCGTCTTGAGACGCTGTTTTGTTTTTCTAGGCACTGTCAGAGTGTT
AACTGAAAGGAGAAGAGGAA

Downstream 100 bases:

>100_bases
TAAACTTATAGAATTGAGAAAAGAGGAGGTCTAGTCTTGCAAAATAAAAGAAACCAATCTAGAGTTCTTTGGCTATTATT
AATATATATAACAATAGGTA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MILQDFEKKIFKFIRDYKIFNKYDKILLGVSGGKDSMSLLHVMSKLSKTMGFEISVAHLNHCMREEADNDEAFVRQACWK
LKIPFFSKKVDVFTYSKKNKVGVEVAGRKLRYEFFYETLRRLSYNKIATAHHMDDLFETMIYRILRGTGIYGLGGLIPIE
EEITKPMLCVDLEEIKNYVTINNIEYVEDKYNYSLDYARNKIRYEITPLFKKINPRYKESFFRLAKIIWSYREEVKRKFE
ERSEISKDSLKLRLENDFFDGEIIRIAFLKFGKYPPNMEETEKIIKMRKGGVRKINGLSITKKSDSLLVKI

Sequences:

>Translated_311_residues
MILQDFEKKIFKFIRDYKIFNKYDKILLGVSGGKDSMSLLHVMSKLSKTMGFEISVAHLNHCMREEADNDEAFVRQACWK
LKIPFFSKKVDVFTYSKKNKVGVEVAGRKLRYEFFYETLRRLSYNKIATAHHMDDLFETMIYRILRGTGIYGLGGLIPIE
EEITKPMLCVDLEEIKNYVTINNIEYVEDKYNYSLDYARNKIRYEITPLFKKINPRYKESFFRLAKIIWSYREEVKRKFE
ERSEISKDSLKLRLENDFFDGEIIRIAFLKFGKYPPNMEETEKIIKMRKGGVRKINGLSITKKSDSLLVKI
>Mature_311_residues
MILQDFEKKIFKFIRDYKIFNKYDKILLGVSGGKDSMSLLHVMSKLSKTMGFEISVAHLNHCMREEADNDEAFVRQACWK
LKIPFFSKKVDVFTYSKKNKVGVEVAGRKLRYEFFYETLRRLSYNKIATAHHMDDLFETMIYRILRGTGIYGLGGLIPIE
EEITKPMLCVDLEEIKNYVTINNIEYVEDKYNYSLDYARNKIRYEITPLFKKINPRYKESFFRLAKIIWSYREEVKRKFE
ERSEISKDSLKLRLENDFFDGEIIRIAFLKFGKYPPNMEETEKIIKMRKGGVRKINGLSITKKSDSLLVKI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=208, Percent_Identity=26.9230769230769, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PETMO (A9BJK2)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001567918.1
- ProteinModelPortal:   A9BJK2
- SMR:   A9BJK2
- GeneID:   5757561
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_0872
- OMA:   YNKIATA
- BioCyc:   PMOB403833:PMOB_0872-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 36882; Mature: 36882

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQDFEKKIFKFIRDYKIFNKYDKILLGVSGGKDSMSLLHVMSKLSKTMGFEISVAHLN
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCEEEHHHHH
HCMREEADNDEAFVRQACWKLKIPFFSKKVDVFTYSKKNKVGVEVAGRKLRYEFFYETLR
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHH
RLSYNKIATAHHMDDLFETMIYRILRGTGIYGLGGLIPIEEEITKPMLCVDLEEIKNYVT
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCEEEHHHHHCEEE
INNIEYVEDKYNYSLDYARNKIRYEITPLFKKINPRYKESFFRLAKIIWSYREEVKRKFE
ECCEEEEHHCCCCCHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSEISKDSLKLRLENDFFDGEIIRIAFLKFGKYPPNMEETEKIIKMRKGGVRKINGLSI
HHHCCCHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCEEE
TKKSDSLLVKI
EECCCCEEEEC
>Mature Secondary Structure
MILQDFEKKIFKFIRDYKIFNKYDKILLGVSGGKDSMSLLHVMSKLSKTMGFEISVAHLN
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCEEEHHHHH
HCMREEADNDEAFVRQACWKLKIPFFSKKVDVFTYSKKNKVGVEVAGRKLRYEFFYETLR
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHH
RLSYNKIATAHHMDDLFETMIYRILRGTGIYGLGGLIPIEEEITKPMLCVDLEEIKNYVT
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCEEEHHHHHCEEE
INNIEYVEDKYNYSLDYARNKIRYEITPLFKKINPRYKESFFRLAKIIWSYREEVKRKFE
ECCEEEEHHCCCCCHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSEISKDSLKLRLENDFFDGEIIRIAFLKFGKYPPNMEETEKIIKMRKGGVRKINGLSI
HHHCCCHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCEEE
TKKSDSLLVKI
EECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA