Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 160901807

GI number: 160901807

Start: 346513

End: 347304

Strand: Direct

Name: trmD [H]

Synonym: Pmob_0323

Alternate gene names: 160901807

Gene position: 346513-347304 (Clockwise)

Preceding gene: 160901806

Following gene: 160901808

Centisome position: 15.97

GC content: 32.45

Gene sequence:

>792_bases
ATGGAACAAATCAAGCGGATTAAAATTAGTGTTTTGACTATTTTCCCTGGTATGTTTGATATAATCAGAAACTATGGAGT
AATCAAAAAAGCTATAGAAAAAAAATTAGTTGAAATAGATATACTCAATTTAAGAGATTACACAACCGATAAACACAAAG
TAACTGATAAACCTGGTTACGGTGGAGAAAATGGAATGGTTATGCTGGCAGAACCTTTTTATCGTTTTTACGATGAATAC
ATACTATCTAAGAACCACAAACCTTATGTAGTTTTACCTTCTCCCCAAGGCGAAGTATTTAATAACGATTTAGCTTTTGA
GTTAGCAAAAAAAGAGGAATTAGTTTTTTTCTGTGGAAGATACGAAGGAATAGATGAAAGGGTTAGAAAGATCGTTGACA
AAGAAGTTTCAATAGGAGATTATGTTTTAACCGGCGGTGAAATCCCTACCATGGTTATTATTGAAACTCTTTTAAGATTT
TTGCCCGGAGTAATAGGTTCAAAAACAAGCGTGGAAAACGATTCCTTTTACAATGGGCTTTTAGATTATTCACACTACAC
CAAACCTCAGAATTTTAGAGGGATGCAAGTGCCGGAAATTCTGTTAAGCGGGGATCATGAACGTATTAGAATCTTTAGAA
AAAAAGACAGTTTGTTAAAAACGATATTGAAGAGGCCAGATCTATTTATAAAGAAAGAATTAGATGAAGAAGAAAAAAAG
ATTTTAGTAGAGATTATTCAAGAAATGTTTAACAAAAACCCTGATAATTTTAAGGAGTCCAAAGATGTTTGA

Upstream 100 bases:

>100_bases
GATGATAAAGAAGTTCTCATACCAATGACAGATCATTACATTATTGAGTTAAAAAAAGAGGATAAAAGTATTATAGTAAA
AAAGATAGAGTGGTACGAAA

Downstream 100 bases:

>100_bases
TAAACTATATGTGGCCTTAATTCACTATCCTATTCTAAAAAAAGACGGAAGCATTGTTTCAACAGCCGTTACAAATTTTG
ATGTTCACGATATTTCAAGA

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MEQIKRIKISVLTIFPGMFDIIRNYGVIKKAIEKKLVEIDILNLRDYTTDKHKVTDKPGYGGENGMVMLAEPFYRFYDEY
ILSKNHKPYVVLPSPQGEVFNNDLAFELAKKEELVFFCGRYEGIDERVRKIVDKEVSIGDYVLTGGEIPTMVIIETLLRF
LPGVIGSKTSVENDSFYNGLLDYSHYTKPQNFRGMQVPEILLSGDHERIRIFRKKDSLLKTILKRPDLFIKKELDEEEKK
ILVEIIQEMFNKNPDNFKESKDV

Sequences:

>Translated_263_residues
MEQIKRIKISVLTIFPGMFDIIRNYGVIKKAIEKKLVEIDILNLRDYTTDKHKVTDKPGYGGENGMVMLAEPFYRFYDEY
ILSKNHKPYVVLPSPQGEVFNNDLAFELAKKEELVFFCGRYEGIDERVRKIVDKEVSIGDYVLTGGEIPTMVIIETLLRF
LPGVIGSKTSVENDSFYNGLLDYSHYTKPQNFRGMQVPEILLSGDHERIRIFRKKDSLLKTILKRPDLFIKKELDEEEKK
ILVEIIQEMFNKNPDNFKESKDV
>Mature_263_residues
MEQIKRIKISVLTIFPGMFDIIRNYGVIKKAIEKKLVEIDILNLRDYTTDKHKVTDKPGYGGENGMVMLAEPFYRFYDEY
ILSKNHKPYVVLPSPQGEVFNNDLAFELAKKEELVFFCGRYEGIDERVRKIVDKEVSIGDYVLTGGEIPTMVIIETLLRF
LPGVIGSKTSVENDSFYNGLLDYSHYTKPQNFRGMQVPEILLSGDHERIRIFRKKDSLLKTILKRPDLFIKKELDEEEKK
ILVEIIQEMFNKNPDNFKESKDV

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=248, Percent_Identity=41.9354838709677, Blast_Score=214, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 30613; Mature: 30613

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQIKRIKISVLTIFPGMFDIIRNYGVIKKAIEKKLVEIDILNLRDYTTDKHKVTDKPGY
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCC
GGENGMVMLAEPFYRFYDEYILSKNHKPYVVLPSPQGEVFNNDLAFELAKKEELVFFCGR
CCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHEEECCCCCEEEEECC
YEGIDERVRKIVDKEVSIGDYVLTGGEIPTMVIIETLLRFLPGVIGSKTSVENDSFYNGL
CCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LDYSHYTKPQNFRGMQVPEILLSGDHERIRIFRKKDSLLKTILKRPDLFIKKELDEEEKK
HHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCHHHHH
ILVEIIQEMFNKNPDNFKESKDV
HHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MEQIKRIKISVLTIFPGMFDIIRNYGVIKKAIEKKLVEIDILNLRDYTTDKHKVTDKPGY
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCC
GGENGMVMLAEPFYRFYDEYILSKNHKPYVVLPSPQGEVFNNDLAFELAKKEELVFFCGR
CCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHEEECCCCCEEEEECC
YEGIDERVRKIVDKEVSIGDYVLTGGEIPTMVIIETLLRFLPGVIGSKTSVENDSFYNGL
CCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LDYSHYTKPQNFRGMQVPEILLSGDHERIRIFRKKDSLLKTILKRPDLFIKKELDEEEKK
HHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCHHHHH
ILVEIIQEMFNKNPDNFKESKDV
HHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]