Click here to switch to the map view.

The map label for this gene is ispA [C]

Identifier: 160901676

GI number: 160901676

Start: 186782

End: 187648

Strand: Direct

Name: ispA [C]

Synonym: Pmob_0188

Alternate gene names: 160901676

Gene position: 186782-187648 (Clockwise)

Preceding gene: 160901675

Following gene: 160901677

Centisome position: 8.61

GC content: 29.18

Gene sequence:

>867_bases
ATGGAATTTCTAAAATTATTAGAGTTTAAAGATTTCTTTGACAAGAAGATAAAGAATTTTTTCGAAAATTTGGACCTGGA
AGATTATTTAAAAGAGCCACTGTTTTATTACATAGAAAATGGAGGCAAAAGACTTAGACCCTGGATAATATATAACTTCG
GACAAATAATCTCTATAAATAAAAAGAATTTAATTGACATTGCGATAGCTGTCGAAATTTTACACTCATCATCCTTGATA
CATGATGATCTACCTGCCTTAGACAATGCAAAGTTAAGAAGAGGAGCTCTAACAACTCATTTAAAGTTTGGAGAATATAA
AGCCATACTAGCTGGAGATTATGGATTCACTCTCCCACTACAGATTGTTGCCGGTTTAGATAATATTAAAGAAGGGAACA
AACTTCTCTTAGTGGATTATTTTATAAAAACCATTTTAAAATTATTTCAAGGAGAAATGGAAGATCTAATCTTCGAAAAA
GAAGATAAAAATGTAGGTGAGAAAGAAATTCTTGAGATGTATTCAAAGAAAACAGGGGCGGTTTTTGGTTTTTGTTTTGC
ATCTCCTTTTTTAATGGCTGGGGAAGCAGAGCTTGCTAAAGAGATGAATAAAATAGGAACTGATTTTGGTGTTTCTTTCC
AAATTTTCGATGATCTAAAAGATATCTTTACTAAAGAAGAAGATATTGGAAAAGAAACTAATAAAGATGTAAACAAAAAA
ACTCTTTTAAATTTTTATAATTTTCAAGAAACACAAATAATAGCCGATAATATTTATATAAATGTTTTACAAAAATTGGA
AGACCTAAATCTAAAAGAACTATCTCAACTTTTGAAAGAAGTTCGAAAGATTATAGAAACAAGGTAA

Upstream 100 bases:

>100_bases
AAACTTCGATATAGACGAACTGAGAAGGAAGTTCGAAAATAAAAATAAAACTGAAGATGAAGATGACGAATAATTTTTTC
TAAAAGCACGAGGTGAATCA

Downstream 100 bases:

>100_bases
AAGTCTCAAAAGGAGTTGACTGAGCTATAAAATTCAAACCTTTCAACTTTTTAAATAAAATTAAATATATGGAAAAAAAA
TACAAAATTCTCTTATCGAT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MEFLKLLEFKDFFDKKIKNFFENLDLEDYLKEPLFYYIENGGKRLRPWIIYNFGQIISINKKNLIDIAIAVEILHSSSLI
HDDLPALDNAKLRRGALTTHLKFGEYKAILAGDYGFTLPLQIVAGLDNIKEGNKLLLVDYFIKTILKLFQGEMEDLIFEK
EDKNVGEKEILEMYSKKTGAVFGFCFASPFLMAGEAELAKEMNKIGTDFGVSFQIFDDLKDIFTKEEDIGKETNKDVNKK
TLLNFYNFQETQIIADNIYINVLQKLEDLNLKELSQLLKEVRKIIETR

Sequences:

>Translated_288_residues
MEFLKLLEFKDFFDKKIKNFFENLDLEDYLKEPLFYYIENGGKRLRPWIIYNFGQIISINKKNLIDIAIAVEILHSSSLI
HDDLPALDNAKLRRGALTTHLKFGEYKAILAGDYGFTLPLQIVAGLDNIKEGNKLLLVDYFIKTILKLFQGEMEDLIFEK
EDKNVGEKEILEMYSKKTGAVFGFCFASPFLMAGEAELAKEMNKIGTDFGVSFQIFDDLKDIFTKEEDIGKETNKDVNKK
TLLNFYNFQETQIIADNIYINVLQKLEDLNLKELSQLLKEVRKIIETR
>Mature_288_residues
MEFLKLLEFKDFFDKKIKNFFENLDLEDYLKEPLFYYIENGGKRLRPWIIYNFGQIISINKKNLIDIAIAVEILHSSSLI
HDDLPALDNAKLRRGALTTHLKFGEYKAILAGDYGFTLPLQIVAGLDNIKEGNKLLLVDYFIKTILKLFQGEMEDLIFEK
EDKNVGEKEILEMYSKKTGAVFGFCFASPFLMAGEAELAKEMNKIGTDFGVSFQIFDDLKDIFTKEEDIGKETNKDVNKK
TLLNFYNFQETQIIADNIYINVLQKLEDLNLKELSQLLKEVRKIIETR

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=236, Percent_Identity=28.3898305084746, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4758430, Length=210, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI83700220, Length=210, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1786623, Length=242, Percent_Identity=31.404958677686, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1789578, Length=211, Percent_Identity=27.4881516587678, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=249, Percent_Identity=23.6947791164659, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325188, Length=206, Percent_Identity=27.1844660194175, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24651612, Length=210, Percent_Identity=25.2380952380952, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281365769, Length=212, Percent_Identity=27.3584905660377, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24660002, Length=212, Percent_Identity=27.3584905660377, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 33387; Mature: 33387

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLKLLEFKDFFDKKIKNFFENLDLEDYLKEPLFYYIENGGKRLRPWIIYNFGQIISIN
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEHHCCCCEECCEEEEECCCEEEEC
KKNLIDIAIAVEILHSSSLIHDDLPALDNAKLRRGALTTHLKFGEYKAILAGDYGFTLPL
CCCHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHCHHHHEEECCCEEEEEECCCCCHHHH
QIVAGLDNIKEGNKLLLVDYFIKTILKLFQGEMEDLIFEKEDKNVGEKEILEMYSKKTGA
HHHHCCHHCCCCCEEEEHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
VFGFCFASPFLMAGEAELAKEMNKIGTDFGVSFQIFDDLKDIFTKEEDIGKETNKDVNKK
EEEHHHHCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCHH
TLLNFYNFQETQIIADNIYINVLQKLEDLNLKELSQLLKEVRKIIETR
HHHHHHCCCHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFLKLLEFKDFFDKKIKNFFENLDLEDYLKEPLFYYIENGGKRLRPWIIYNFGQIISIN
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEHHCCCCEECCEEEEECCCEEEEC
KKNLIDIAIAVEILHSSSLIHDDLPALDNAKLRRGALTTHLKFGEYKAILAGDYGFTLPL
CCCHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHCHHHHEEECCCEEEEEECCCCCHHHH
QIVAGLDNIKEGNKLLLVDYFIKTILKLFQGEMEDLIFEKEDKNVGEKEILEMYSKKTGA
HHHHCCHHCCCCCEEEEHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
VFGFCFASPFLMAGEAELAKEMNKIGTDFGVSFQIFDDLKDIFTKEEDIGKETNKDVNKK
EEEHHHHCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCHH
TLLNFYNFQETQIIADNIYINVLQKLEDLNLKELSQLLKEVRKIIETR
HHHHHHCCCHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]