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The map label for this gene is nqrF [H]

Identifier: 160901557

GI number: 160901557

Start: 61785

End: 62903

Strand: Direct

Name: nqrF [H]

Synonym: Pmob_0066

Alternate gene names: 160901557

Gene position: 61785-62903 (Clockwise)

Preceding gene: 160901556

Following gene: 160901558

Centisome position: 2.85

GC content: 36.28

Gene sequence:

>1119_bases
GTGAACGCAATTTTAGTTGCTTCATTTATAATAGGTGGATTGAGTGCGGTTTTAGCTGCTATGATTGTGGTTGTTGATAG
CGTAGTTAACAATTATGGTGAAGTTAAGATCGATATCAATAATGGAAAAAAGGAGTTAAAGGTAAATGGAGGCGCTCCAC
TACTTACTACTCTTTCCGAGCAAGGCATCTTTATCCCTTCAGCATGTGGTGGTAGGGGAAGTTGTGGGGCATGCAAGGTA
AAAGTTTTATCAGATATAGGACCAATTTTACCCACGGAAGCGCCACTTTTAGACGAGGAAGAAATGAAACAAAATATAAG
ATTATCATGTCAAGTGAAAGTAAAATCTGACATAGCGATAGAAATTCCAGAAGAGTTATTTTCTGCAAAAATTTTTAAAG
GTGTGGTTGAAAAAATAAATGATTTGACATATGATATAAAAGAAGTTAAAATTAAACTTGTCGAGCCCAATGAAATCGAG
TTTAAAGCTGGACAGTATATGCAATTGGTTATACCTCCCTACGAGAAAATAAACGAATATACACAAAGGGCATATTCCAT
TGCATCGTCGCCAAGTCAAAAAGATTCCATAGAATTTTTTATCAGACTTGTTCCAGGTGGAATTGCGACAACTTATGTTC
ACAAATATCTAAAAGAAAATGATCAAATGGAATTGGTTGGTCCTTTCGGTGAATTTTATATGAGAGATACGGATGCAGAT
ATGATATGTGTTGCAGGAGGTTCAGGGTTAGCTCCGATAAAATCTATCGTTGCAGATATGTTCGAAAGGGAAATAACCAA
CAGAAACGTCTGGTTGTTTTTCGGGGCAAGAAGCTTGAAAGATCTTTATTATCTGGATTTCTTTCAAGACATGGAAAAGA
AATGGGACAGATTCCATTTTGTTCCTGCACTTTCAGAACCACAACCTGAAGATAATTGGAAAGGAGAAACGGGACTTATA
ACAGATGTATTGGGAAAATATTTTAAAGAAAAAATGGACCAAAATACCCAAAAGGAAGGATATTTATGTGGAAGCCCCGG
TATGATAAATGCCTGTGTTAAAGTAATGACAGCGAACGGCATATCCGAGGATAAAATATATTATGACAAGTTCGCGTAA

Upstream 100 bases:

>100_bases
CCGTTAAAAGGACCTGGAATTACCTTAATCACGATTGGACTTATGGCGATGGCTTTTATGGGATTTTCCGGTATGCTAAA
CGTACAGTGAGGTGATTGAT

Downstream 100 bases:

>100_bases
TTAAAAGGAGGTGTTTTTCCTCAATGAAAATGACTGAAGATTTAAAGAAAGCCTATGAAAACATGAAGCCCGGAGTTATA
ACCGCTGATGGATTTTTAGG

Product: oxidoreductase FAD/NAD(P)-binding subunit

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNAILVASFIIGGLSAVLAAMIVVVDSVVNNYGEVKIDINNGKKELKVNGGAPLLTTLSEQGIFIPSACGGRGSCGACKV
KVLSDIGPILPTEAPLLDEEEMKQNIRLSCQVKVKSDIAIEIPEELFSAKIFKGVVEKINDLTYDIKEVKIKLVEPNEIE
FKAGQYMQLVIPPYEKINEYTQRAYSIASSPSQKDSIEFFIRLVPGGIATTYVHKYLKENDQMELVGPFGEFYMRDTDAD
MICVAGGSGLAPIKSIVADMFEREITNRNVWLFFGARSLKDLYYLDFFQDMEKKWDRFHFVPALSEPQPEDNWKGETGLI
TDVLGKYFKEKMDQNTQKEGYLCGSPGMINACVKVMTANGISEDKIYYDKFA

Sequences:

>Translated_372_residues
MNAILVASFIIGGLSAVLAAMIVVVDSVVNNYGEVKIDINNGKKELKVNGGAPLLTTLSEQGIFIPSACGGRGSCGACKV
KVLSDIGPILPTEAPLLDEEEMKQNIRLSCQVKVKSDIAIEIPEELFSAKIFKGVVEKINDLTYDIKEVKIKLVEPNEIE
FKAGQYMQLVIPPYEKINEYTQRAYSIASSPSQKDSIEFFIRLVPGGIATTYVHKYLKENDQMELVGPFGEFYMRDTDAD
MICVAGGSGLAPIKSIVADMFEREITNRNVWLFFGARSLKDLYYLDFFQDMEKKWDRFHFVPALSEPQPEDNWKGETGLI
TDVLGKYFKEKMDQNTQKEGYLCGSPGMINACVKVMTANGISEDKIYYDKFA
>Mature_372_residues
MNAILVASFIIGGLSAVLAAMIVVVDSVVNNYGEVKIDINNGKKELKVNGGAPLLTTLSEQGIFIPSACGGRGSCGACKV
KVLSDIGPILPTEAPLLDEEEMKQNIRLSCQVKVKSDIAIEIPEELFSAKIFKGVVEKINDLTYDIKEVKIKLVEPNEIE
FKAGQYMQLVIPPYEKINEYTQRAYSIASSPSQKDSIEFFIRLVPGGIATTYVHKYLKENDQMELVGPFGEFYMRDTDAD
MICVAGGSGLAPIKSIVADMFEREITNRNVWLFFGARSLKDLYYLDFFQDMEKKWDRFHFVPALSEPQPEDNWKGETGLI
TDVLGKYFKEKMDQNTQKEGYLCGSPGMINACVKVMTANGISEDKIYYDKFA

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=242, Percent_Identity=28.5123966942149, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 41467; Mature: 41467

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAILVASFIIGGLSAVLAAMIVVVDSVVNNYGEVKIDINNGKKELKVNGGAPLLTTLSE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCEEEECCC
QGIFIPSACGGRGSCGACKVKVLSDIGPILPTEAPLLDEEEMKQNIRLSCQVKVKSDIAI
CCEEEECCCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCHHHHHCCCEEEEEEEEECCEEE
EIPEELFSAKIFKGVVEKINDLTYDIKEVKIKLVEPNEIEFKAGQYMQLVIPPYEKINEY
ECCHHHHHHHHHHHHHHHHHHHHEEEEHEEEEEECCCCEEECCCCEEEEEECCHHHHHHH
TQRAYSIASSPSQKDSIEFFIRLVPGGIATTYVHKYLKENDQMELVGPFGEFYMRDTDAD
HHHHHHHHCCCCCCHHHEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCHHHEEECCCCC
MICVAGGSGLAPIKSIVADMFEREITNRNVWLFFGARSLKDLYYLDFFQDMEKKWDRFHF
EEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEE
VPALSEPQPEDNWKGETGLITDVLGKYFKEKMDQNTQKEGYLCGSPGMINACVKVMTANG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCC
ISEDKIYYDKFA
CCCCCEEECCCC
>Mature Secondary Structure
MNAILVASFIIGGLSAVLAAMIVVVDSVVNNYGEVKIDINNGKKELKVNGGAPLLTTLSE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCEEEECCC
QGIFIPSACGGRGSCGACKVKVLSDIGPILPTEAPLLDEEEMKQNIRLSCQVKVKSDIAI
CCEEEECCCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCHHHHHCCCEEEEEEEEECCEEE
EIPEELFSAKIFKGVVEKINDLTYDIKEVKIKLVEPNEIEFKAGQYMQLVIPPYEKINEY
ECCHHHHHHHHHHHHHHHHHHHHEEEEHEEEEEECCCCEEECCCCEEEEEECCHHHHHHH
TQRAYSIASSPSQKDSIEFFIRLVPGGIATTYVHKYLKENDQMELVGPFGEFYMRDTDAD
HHHHHHHHCCCCCCHHHEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCHHHEEECCCCC
MICVAGGSGLAPIKSIVADMFEREITNRNVWLFFGARSLKDLYYLDFFQDMEKKWDRFHF
EEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEE
VPALSEPQPEDNWKGETGLITDVLGKYFKEKMDQNTQKEGYLCGSPGMINACVKVMTANG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCC
ISEDKIYYDKFA
CCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA