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The map label for this gene is trmE [H]

Identifier: 160877621

GI number: 160877621

Start: 5343370

End: 5344731

Strand: Reverse

Name: trmE [H]

Synonym: Sbal195_4520

Alternate gene names: 160877621

Gene position: 5344731-5343370 (Counterclockwise)

Preceding gene: 160877622

Following gene: 160877620

Centisome position: 99.95

GC content: 47.87

Gene sequence:

>1362_bases
GTGACAACTGACACTATCGTGGCTCAAGCCACCGCGCCCGGACGTGGCGGCGTTGGCATCATTCGAATTTCAGGCGACAA
GGCCAGTGATGTCGCTATGGCCGTATTAGGTCATCTTCCTAAAACCCGATATGCCGATTATTGCGATTTTAAAAGTGCAT
CGGGCCAAGTAATAGACCAAGGCATAGCACTGTTTTTTAAAGGACCGAATTCCTTTACTGGCGAAGATGTCTTGGAACTT
CAAGGCCACGGCGGCCAAATTGTCCTAGACATGCTGATCAAACGCGTAATGGAAGTCGGTGGCATTCGTATCGCAAAACC
AGGTGAGTTCAGCGAACAAGCCTTTATGAACGATAAACTCGACTTAACCCAAGCCGAAGCCATTGCCGACCTTATCGACG
CCACCAGTGAGCAAGCCGCAAAAAGTGCGTTGCAATCACTACAAGGTGAATTTTCTAAGGAAGTCCACGAGCTGGTGGAC
CAAGTCACTAACCTGCGTTTATACGTTGAAGCCGCAATTGATTTCCCCGATGAAGAAGTCGACTTTTTAAGTGACGGCAA
AATTGCCAATGCACTCTATAAAATCATCGACAAACTTGACCTAGTGCAAGCCAGTGCCAAGCAAGGTTCTATCATTCGTG
AAGGGATGAAAGTGGTGATTGCCGGTCGTCCCAATGCCGGAAAATCGAGCTTACTGAATGCGTTAGCGGGTAAAGAGTCG
GCCATTGTGACTGAAATTGCCGGTACAACACGCGACGTATTACGTGAACATATCCATTTAGATGGTATGCCGCTGCACAT
TATTGATACCGCAGGCTTGCGTGATACCAATGATACGGTAGAACAAATTGGTATTGAACGCGCATGGAATGAAATAAACA
GCGCCGATCGTGTGCTATTTATGGTGGATGGCACGACAACAGCCGCCGTCGATCCCCATACGATTTGGCCTGACTTTGTC
GATCGCCTGCCCTCCAATTTAGGTGTAACAGTGATCCGTAATAAAGCCGATCTCACGGGCGAAGACCTAATGATGACTGA
AGAACAAGGTTATAGCGTCTACCGGATCTCGGCTAAAACAGGCTTAGGTGTTGAAGAATTAAAGCAACACTTGAAGTCAC
TTATGGGTTATCAGAGTAATCTCGAAGGTGGCTTTATCGCCCGTCGTCGTCACTTAGAAGCCCTTGAATTAGCCGCAGGA
CACCTGCAATTGGGTAAAGAGCAGTTAGAGGTTTACCTTGCCGGAGAGCTGCTGGCGGAAGAGCTACGTATGTGCCAATT
AGCACTATCTGAGATCACAGGCCGCTTCACTTCTGACGATTTACTCGGCAAGATTTTCAGCTCTTTCTGTATCGGGAAGT
AA

Upstream 100 bases:

>100_bases
TCGTTACAGATTAATGTGAACGAATGACACTAAAAGGCGGCTTAATTGGCCGCCTTTTCCTTTACATATTTTAGTAGATA
TTAGTTTGGTGGGTATTTCC

Downstream 100 bases:

>100_bases
TGTTCTCGCCATGTGAACTGCTTTTCTAGCTAAGGCTTGTTGTGCTCAACAAGCCTTTCTTTTAACTAAATCCTCGTTAT
CCCGCTTATATATCTTCCTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTDTIVAQATAPGRGGVGIIRISGDKASDVAMAVLGHLPKTRYADYCDFKSASGQVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVD
QVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTNDTVEQIGIERAWNEINSADRVLFMVDGTTTAAVDPHTIWPDFV
DRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEALELAAG
HLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_453_residues
MTTDTIVAQATAPGRGGVGIIRISGDKASDVAMAVLGHLPKTRYADYCDFKSASGQVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVD
QVTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTNDTVEQIGIERAWNEINSADRVLFMVDGTTTAAVDPHTIWPDFV
DRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEALELAAG
HLQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK
>Mature_452_residues
TTDTIVAQATAPGRGGVGIIRISGDKASDVAMAVLGHLPKTRYADYCDFKSASGQVIDQGIALFFKGPNSFTGEDVLELQ
GHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVDQ
VTNLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLDLVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKESA
IVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTNDTVEQIGIERAWNEINSADRVLFMVDGTTTAAVDPHTIWPDFVD
RLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKTGLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEALELAAGH
LQLGKEQLEVYLAGELLAEELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.0425531914894, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=34.1304347826087, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=31.6733067729084, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=72.7272727272727, Blast_Score=675, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=36.9565217391304, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=29.4243070362473, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.1871227364185, Blast_Score=202, Evalue=9e-53,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=32.4894514767932, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49174; Mature: 49042

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIVAQATAPGRGGVGIIRISGDKASDVAMAVLGHLPKTRYADYCDFKSASGQVIDQ
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVDQVTNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIANALYKIIDKLDLVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTNDTVEQIGIERAWNEINSADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
MVDGTTTAAVDPHTIWPDFVDRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKT
EECCCEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEECCC
GLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEALELAAGHLQLGKEQLEVYLAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTDTIVAQATAPGRGGVGIIRISGDKASDVAMAVLGHLPKTRYADYCDFKSASGQVIDQ
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVGGIRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVDQVTNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIANALYKIIDKLDLVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTNDTVEQIGIERAWNEINSADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
MVDGTTTAAVDPHTIWPDFVDRLPSNLGVTVIRNKADLTGEDLMMTEEQGYSVYRISAKT
EECCCEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEECCC
GLGVEELKQHLKSLMGYQSNLEGGFIARRRHLEALELAAGHLQLGKEQLEVYLAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ELRMCQLALSEITGRFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA