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The map label for this gene is uvrD [H]

Identifier: 160877520

GI number: 160877520

Start: 5220834

End: 5222504

Strand: Direct

Name: uvrD [H]

Synonym: Sbal195_4419

Alternate gene names: 160877520

Gene position: 5220834-5222504 (Clockwise)

Preceding gene: 160877519

Following gene: 160877521

Centisome position: 97.64

GC content: 40.1

Gene sequence:

>1671_bases
GTGATTAGACACGATAACTGGAAACCTGCGGATGGGCTAACTCTTGAACCAAATGCTGAACGAGCAGTAAAAGAATGCGA
AAAAAATTTAGCCCTAACAGCAGGTCCTGGTGCTGGTAAAACAGAAATGCTTGCTCAGCGAGCTGACTTCTTACTCCGAA
CAGGCACTTGCCGCTATCCGAAGCGAATTTTGGCAATTTCATTTAAAGTTGATGCCAGTTCAAACCTAAAAGATCGGGTA
ACTCGCAGGAGTGGAGATGAATTATCATCTCGATTTGATAGTTACACGTTTCATGCATTTGCTAAGCGAATTATTGACCG
TTTTCGCCCCATACTCACAGGCATCTACACGTTAGATTCAGAATATACCATTGGAAGTCCTAGAACGCCTCAGAAGCAAA
TAGAATTTGCAGATTTGGTTCCATTAGCAACTCGTATCCTCCAAAGTTCTGCTGTAGCTAGAAATGCCATAAGACAAACA
TATACTGATGTGTTCTTGGATGAATTTCAGGATTGTACAGATATTCAGTATGAGTTTCTAAAAATAGCTTTCCAAAGCAC
AACAATTCGATTAACAGCTGTTGGAGATACGAAGCAAATAATTATGGGGTGGGCAGGTGCGCTTGATGGGATTTTTGTCA
CTTATGCTCAAGATTTTCAAGCATTACCATTAAACATGTATCGTAACTTCCGCTCAAAGCCACAGCTTCTTCGGTTACAA
AACGAAATCATCCGGACACTAGATCCTGCCTCGGTGATGCCGAATGAACTCATTGTTGGCAATGAAGGAGAAATACGAGC
ATTTCAATTTGAAACAAGCCAACGTGAAGCTGAATATCTAGCCGAACAAATTCAAAATTGGATAATCGTAGATAATATCC
CTCCAAACGAGATTGCTATACTCATACGAAATCAACCACATTTATATGCCCAACATCTAATACCTGCGCTGCGAAATAGA
CAAATACCTTTTAGAAACGAGCAAGAATTACAGGATATAACTGTGGAACCTGCTGCCCGCCTCATTGTCGATTTCCTTCT
TTGTACTTTTGGTAAACGTGAACCCGCAGCTTGGTTAAGGCTAATGAACCAATTGATCCCATTTACCGATGACGAAGAAG
AGCAGGATAAATATCAGCAAATCTGGCTTAACTTCATTCAACGCCAGCGGAAAAGAATTAGGGAAAACCCAATAACAATA
GATAACCAAAGTCGTTGGCAAAGTGTTCAAAGTTTTATTGAACTTGTCGGTATTGAAACAATGACAGCACTTTCACATGA
CTACGAAACAGCCTCTCGTCTAAATGAAGTTCTAAGGGCAACATTTAATAAGTTAGATGAAACCTTGGCTTCAGGCCTGA
CACTACTTGAAGCACTTTGCCAGTTTACAGATGATCAATCAGTAAGAATATTGACCTCACACAAAAGTAAAGGGCTAGAG
TTTGATACTGTAGTACTTTTAGGCATCGAAAATGAATGTTACTTTGGGGCCCCTCAAGAGAACCTATGTACCTTTTTTGT
TGGAGTTTCCAGAGCTAAATGTAAGCTATTGTTAACATTTGCAAAAGAGCGAATACGGCCAGAAGGCTTTTCTGGTCGGT
GGAGTGAACAAAGAACACTTGTTAATGAGTATGCTGGTTATGTCAGGCCATTTTTATCTAACAATAACTAA

Upstream 100 bases:

>100_bases
CACTGTCCAACATTTCCAATACACAGCTTCTTGCCTCAATACCGCCGTCATTAGGCCGATTAGCAGACGCCGTTATCGCT
AAACTAGCGGAGTTACCAGA

Downstream 100 bases:

>100_bases
ATTAAATAGTGACTTATGATATACGATGGGTTTGCTTACAATGACAGCATGAAAGATACAATTAAACTCTAATACTACGT
TGCTTACTTGAAAAATTGTG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MIRHDNWKPADGLTLEPNAERAVKECEKNLALTAGPGAGKTEMLAQRADFLLRTGTCRYPKRILAISFKVDASSNLKDRV
TRRSGDELSSRFDSYTFHAFAKRIIDRFRPILTGIYTLDSEYTIGSPRTPQKQIEFADLVPLATRILQSSAVARNAIRQT
YTDVFLDEFQDCTDIQYEFLKIAFQSTTIRLTAVGDTKQIIMGWAGALDGIFVTYAQDFQALPLNMYRNFRSKPQLLRLQ
NEIIRTLDPASVMPNELIVGNEGEIRAFQFETSQREAEYLAEQIQNWIIVDNIPPNEIAILIRNQPHLYAQHLIPALRNR
QIPFRNEQELQDITVEPAARLIVDFLLCTFGKREPAAWLRLMNQLIPFTDDEEEQDKYQQIWLNFIQRQRKRIRENPITI
DNQSRWQSVQSFIELVGIETMTALSHDYETASRLNEVLRATFNKLDETLASGLTLLEALCQFTDDQSVRILTSHKSKGLE
FDTVVLLGIENECYFGAPQENLCTFFVGVSRAKCKLLLTFAKERIRPEGFSGRWSEQRTLVNEYAGYVRPFLSNNN

Sequences:

>Translated_556_residues
MIRHDNWKPADGLTLEPNAERAVKECEKNLALTAGPGAGKTEMLAQRADFLLRTGTCRYPKRILAISFKVDASSNLKDRV
TRRSGDELSSRFDSYTFHAFAKRIIDRFRPILTGIYTLDSEYTIGSPRTPQKQIEFADLVPLATRILQSSAVARNAIRQT
YTDVFLDEFQDCTDIQYEFLKIAFQSTTIRLTAVGDTKQIIMGWAGALDGIFVTYAQDFQALPLNMYRNFRSKPQLLRLQ
NEIIRTLDPASVMPNELIVGNEGEIRAFQFETSQREAEYLAEQIQNWIIVDNIPPNEIAILIRNQPHLYAQHLIPALRNR
QIPFRNEQELQDITVEPAARLIVDFLLCTFGKREPAAWLRLMNQLIPFTDDEEEQDKYQQIWLNFIQRQRKRIRENPITI
DNQSRWQSVQSFIELVGIETMTALSHDYETASRLNEVLRATFNKLDETLASGLTLLEALCQFTDDQSVRILTSHKSKGLE
FDTVVLLGIENECYFGAPQENLCTFFVGVSRAKCKLLLTFAKERIRPEGFSGRWSEQRTLVNEYAGYVRPFLSNNN
>Mature_556_residues
MIRHDNWKPADGLTLEPNAERAVKECEKNLALTAGPGAGKTEMLAQRADFLLRTGTCRYPKRILAISFKVDASSNLKDRV
TRRSGDELSSRFDSYTFHAFAKRIIDRFRPILTGIYTLDSEYTIGSPRTPQKQIEFADLVPLATRILQSSAVARNAIRQT
YTDVFLDEFQDCTDIQYEFLKIAFQSTTIRLTAVGDTKQIIMGWAGALDGIFVTYAQDFQALPLNMYRNFRSKPQLLRLQ
NEIIRTLDPASVMPNELIVGNEGEIRAFQFETSQREAEYLAEQIQNWIIVDNIPPNEIAILIRNQPHLYAQHLIPALRNR
QIPFRNEQELQDITVEPAARLIVDFLLCTFGKREPAAWLRLMNQLIPFTDDEEEQDKYQQIWLNFIQRQRKRIRENPITI
DNQSRWQSVQSFIELVGIETMTALSHDYETASRLNEVLRATFNKLDETLASGLTLLEALCQFTDDQSVRILTSHKSKGLE
FDTVVLLGIENECYFGAPQENLCTFFVGVSRAKCKLLLTFAKERIRPEGFSGRWSEQRTLVNEYAGYVRPFLSNNN

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=655, Percent_Identity=23.206106870229, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 63904; Mature: 63904

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRHDNWKPADGLTLEPNAERAVKECEKNLALTAGPGAGKTEMLAQRADFLLRTGTCRYP
CCCCCCCCCCCCCEECCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
KRILAISFKVDASSNLKDRVTRRSGDELSSRFDSYTFHAFAKRIIDRFRPILTGIYTLDS
CEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
EYTIGSPRTPQKQIEFADLVPLATRILQSSAVARNAIRQTYTDVFLDEFQDCTDIQYEFL
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KIAFQSTTIRLTAVGDTKQIIMGWAGALDGIFVTYAQDFQALPLNMYRNFRSKPQLLRLQ
HHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEHHCCCCCCHHHHHCCCCCHHHHHHH
NEIIRTLDPASVMPNELIVGNEGEIRAFQFETSQREAEYLAEQIQNWIIVDNIPPNEIAI
HHHHHHCCCHHCCCCCEEECCCCCEEEEEECCHHHHHHHHHHHHCCEEEECCCCCCCEEE
LIRNQPHLYAQHLIPALRNRQIPFRNEQELQDITVEPAARLIVDFLLCTFGKREPAAWLR
EECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHH
LMNQLIPFTDDEEEQDKYQQIWLNFIQRQRKRIRENPITIDNQSRWQSVQSFIELVGIET
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
MTALSHDYETASRLNEVLRATFNKLDETLASGLTLLEALCQFTDDQSVRILTSHKSKGLE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
FDTVVLLGIENECYFGAPQENLCTFFVGVSRAKCKLLLTFAKERIRPEGFSGRWSEQRTL
EEEEEEEEECCCCEECCCHHHHEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VNEYAGYVRPFLSNNN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRHDNWKPADGLTLEPNAERAVKECEKNLALTAGPGAGKTEMLAQRADFLLRTGTCRYP
CCCCCCCCCCCCCEECCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
KRILAISFKVDASSNLKDRVTRRSGDELSSRFDSYTFHAFAKRIIDRFRPILTGIYTLDS
CEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
EYTIGSPRTPQKQIEFADLVPLATRILQSSAVARNAIRQTYTDVFLDEFQDCTDIQYEFL
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KIAFQSTTIRLTAVGDTKQIIMGWAGALDGIFVTYAQDFQALPLNMYRNFRSKPQLLRLQ
HHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEHHCCCCCCHHHHHCCCCCHHHHHHH
NEIIRTLDPASVMPNELIVGNEGEIRAFQFETSQREAEYLAEQIQNWIIVDNIPPNEIAI
HHHHHHCCCHHCCCCCEEECCCCCEEEEEECCHHHHHHHHHHHHCCEEEECCCCCCCEEE
LIRNQPHLYAQHLIPALRNRQIPFRNEQELQDITVEPAARLIVDFLLCTFGKREPAAWLR
EECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHH
LMNQLIPFTDDEEEQDKYQQIWLNFIQRQRKRIRENPITIDNQSRWQSVQSFIELVGIET
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
MTALSHDYETASRLNEVLRATFNKLDETLASGLTLLEALCQFTDDQSVRILTSHKSKGLE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
FDTVVLLGIENECYFGAPQENLCTFFVGVSRAKCKLLLTFAKERIRPEGFSGRWSEQRTL
EEEEEEEEECCCCEECCCHHHHEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VNEYAGYVRPFLSNNN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]