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The map label for this gene is fdhB1 [H]

Identifier: 160877327

GI number: 160877327

Start: 5000663

End: 5001259

Strand: Direct

Name: fdhB1 [H]

Synonym: Sbal195_4225

Alternate gene names: 160877327

Gene position: 5000663-5001259 (Clockwise)

Preceding gene: 160877326

Following gene: 160877328

Centisome position: 93.52

GC content: 49.92

Gene sequence:

>597_bases
ATGGCAGTCATGAAATTTTTGTGTGACACCAAGCGCTGCATCGAGTGTAACGGTTGTGTCACTGCATGTAAGAACGAAAA
CGACTCCGCTCTCGAGTGGGGAATCCAACGTCGCCGCGTGGTGACGATTAACGATGGTCTACCTGGCGAAGCTTCTATTT
CAGTGGCATGTATGCACTGTACTGACGCTCCCTGCATGGCTGTATGTCCGGCAGATTGTTTCTACCGTACCGAAGACGGC
ATAGTGCTACACAACAAAGATACCTGTATCGGTTGTGGTTATTGCTTCTATGCTTGTCCATTTGGTGCACCGCAATTCCC
GAAAAAGACTGCCTTTGGTAGTCGTGGCAAGATGGACAAGTGTACTTTCTGCGCCGGTGGCCCAGAAGAAACCTTCTCCG
AAGCCGAGCGTAAAAAATACGGTGCCAACCGTATTGCTGAGGGCAAGTTACCTATGTGTGCTGAGCTTTGTTCGACAAAA
GCCCTGCTCGCGGGTGATGCGGAAGTGGTATCCAATATCTACCGTCAACGTATGGCCTCTCGTGGCAATCCTAACGTGAT
TTGGGGTTACAACCCCAAGACAGGTGAGATTAACTAA

Upstream 100 bases:

>100_bases
TAACTTATGGCTACGACGTTGTGACTCAGATGCAAGAAACTAAGTCCAGCTTGTGCCAGATCAGCAAAGCCTAACCTACT
TGATGAGGAGATAAGCCATT

Downstream 100 bases:

>100_bases
GCAGAGCGAGGGTTAGGGAGTCATCCCTAACCTCTGGCAAGGAGTGAAAATGTTAAACAAACAGTTAAACAAATCACTGC
GCAGTCTGTTTGCTCTGTTG

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGLPGEASISVACMHCTDAPCMAVCPADCFYRTEDG
IVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDKCTFCAGGPEETFSEAERKKYGANRIAEGKLPMCAELCSTK
ALLAGDAEVVSNIYRQRMASRGNPNVIWGYNPKTGEIN

Sequences:

>Translated_198_residues
MAVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGLPGEASISVACMHCTDAPCMAVCPADCFYRTEDG
IVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDKCTFCAGGPEETFSEAERKKYGANRIAEGKLPMCAELCSTK
ALLAGDAEVVSNIYRQRMASRGNPNVIWGYNPKTGEIN
>Mature_197_residues
AVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGLPGEASISVACMHCTDAPCMAVCPADCFYRTEDGI
VLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDKCTFCAGGPEETFSEAERKKYGANRIAEGKLPMCAELCSTKA
LLAGDAEVVSNIYRQRMASRGNPNVIWGYNPKTGEIN

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=171, Percent_Identity=34.5029239766082, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787872, Length=174, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=28.7128712871287, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510944, Length=190, Percent_Identity=32.1052631578947, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1790326, Length=201, Percent_Identity=29.3532338308458, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787749, Length=192, Percent_Identity=28.6458333333333, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI2367345, Length=200, Percent_Identity=31.5, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI2367245, Length=184, Percent_Identity=31.5217391304348, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788811, Length=126, Percent_Identity=30.952380952381, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082180, Length=185, Percent_Identity=29.1891891891892, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789079, Length=100, Percent_Identity=37, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082114, Length=187, Percent_Identity=27.807486631016, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 21591; Mature: 21460

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
12.6 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGLPGEASISVACMHC
CHHHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEC
TDAPCMAVCPADCFYRTEDGIVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDK
CCCCEEEECCCHHEEECCCCEEEECCCCEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEETFSEAERKKYGANRIAEGKLPMCAELCSTKALLAGDAEVVSNIYRQRMAS
EEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHC
RGNPNVIWGYNPKTGEIN
CCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
AVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGLPGEASISVACMHC
HHHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEC
TDAPCMAVCPADCFYRTEDGIVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDK
CCCCEEEECCCHHEEECCCCEEEECCCCEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEETFSEAERKKYGANRIAEGKLPMCAELCSTKALLAGDAEVVSNIYRQRMAS
EEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHC
RGNPNVIWGYNPKTGEIN
CCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]