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The map label for this gene is nfdA [H]

Identifier: 160877091

GI number: 160877091

Start: 4692458

End: 4694149

Strand: Direct

Name: nfdA [H]

Synonym: Sbal195_3987

Alternate gene names: 160877091

Gene position: 4692458-4694149 (Clockwise)

Preceding gene: 160877090

Following gene: 160877094

Centisome position: 87.75

GC content: 43.85

Gene sequence:

>1692_bases
ATGAAACAGACTAAGACGCCACACAAACTCGTTAAATGCTTAGCCTTAACGGGATTATTCACCCTATCTGCCTGTGCAAA
CGACGAACTTGCAGATACTTTACTCACCAATACCCACGTCTATGGCCATGATCAAGCGACTAGTTTAGCCATTAAGGATG
GCAAAATTGTCTACATTGGGAATAGCATTAACGCCATGGATCACGTGAGCAATCAAACCAAAGTCATCGATTTAAAAGGT
CATTACTTATTACCTGGTTTCATTGATAACCATAATCATGTGTTTGAAGCCGCGAGTGAAATAGGTAGCGACTGTTTACT
GGATGATGACACTAATCTCAACGGCTATATCCGCACATTAACCGCCTGTAAAAATCAGTTGGAAACGGGAGAGTGGTTAC
TCGGCTATGGCCATCAACTCAGTGTAGTAATGCAAGAGAATGGCCCCACTCCTTTGGCAATATTAGACAAAATCTTCCCC
GATAATCCTGTCGTTATTATGGAGCAAACATCCCATTCTATGTGGGTCAACTCCCTCGCATTAGCCAAAGCTAAGATAAG
CGCTCATACAGCCGATCCGCAAGGCGGCAAGATCCTCAAAGATGAAGAGAGCGGTAAACTTCTCGGTATTTTATTCGATA
ATGCTGGCGATCTTGTGATGGAACAAGCATGGAAATCACAACCCAATCTACAACAAGCCAATTATCAAGGTTTATTAAAT
GGTCTGGCAGCTGCTGCCGAGCACGGGATAACGACTATCGGTGATGGACGACTCTATTGGAAACGTGACTGGTTAGAAAC
ATGGCTAGCCGTCGAAAGTCATGATGAATTAACCGCCAGAGTCAGTCTACGCCCTTGGATTTATCCTGCCGATGAACCCC
AAGCACAGCTTGATTTCTTTAAAAAAATTCATTCTGATGATAAACAACGTCTGCTGCTCATCAATCAGGTCAAAATCTAC
AGTGATGGCATCATCATAAACGGTACTGCCAGTACATTAGCCCCCTATGATTGGAGCTACACCCCCGAACTTCCTAGGGG
ACTCGAGTATGTTCCTGCCAGTAAAATGACGTGGTGGCTTACTGAACTCAACAAAATTGGATACGGGGCACATATTCATG
CCATAGGTGATGCAGGGGTACGCAACACCTTAGATGCTATTGAAACTTTACGATCTCAGGGAGCTACTCGCCCTTATGCC
ATGACGCATTTAGAAATGGTATCTCCCCAAGATATCAAACGCTTTGCCTCACTAAAAGTCGATGCCGATTTCCAATTGGG
TAGCGATTACATTGCTGCGCAGGATCATGAGTGGGCGATCCCTTTTATCGGTAAGCAACGAGCTCATGCTTTATTACCTA
TTAACCCTATTTTCAAGACTGGCGCGAATGTAACTTTAAGTAGTGACTGGAATGTGAATGATATCAATCCACTCGTTGGT
ATTGCTAACGCAGTCTATCTGCAAAATGCAGGCATCACCGATATTCACAGTGCTATTGCAGCCTATACAATAAATCCCGC
TAACGCCTTGGGCATAAGTGACATTACTGGTTCTATCGTGCTAGGGAAATCTGCCGATTTTGTTGTATTAGAGCGAGACA
TTACCCAAGCTACAGCAAAACAAATTGCTGACACTCAAATTTTAATGACGTGGTTAGAGGGGGAGCTAGTGTTCGATCTC
AATGATCAATAG

Upstream 100 bases:

>100_bases
CCCATGGGTATCTCATTTTCTTGATAGTCACTAACTTTCACTATTGTGGAATTATCCCATAATGAGTCAATAGCTGACGC
AACATGGGATTCAAGTACAA

Downstream 100 bases:

>100_bases
CAATCCCGTATCAATCTAATTATTTGCTGAGTTGCTCTTTTATTAACGTCAGCAAAGAAGACCCTAAGGTGCTGGCCTCA
AACCCATGATGCCAGCGCAT

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKQTKTPHKLVKCLALTGLFTLSACANDELADTLLTNTHVYGHDQATSLAIKDGKIVYIGNSINAMDHVSNQTKVIDLKG
HYLLPGFIDNHNHVFEAASEIGSDCLLDDDTNLNGYIRTLTACKNQLETGEWLLGYGHQLSVVMQENGPTPLAILDKIFP
DNPVVIMEQTSHSMWVNSLALAKAKISAHTADPQGGKILKDEESGKLLGILFDNAGDLVMEQAWKSQPNLQQANYQGLLN
GLAAAAEHGITTIGDGRLYWKRDWLETWLAVESHDELTARVSLRPWIYPADEPQAQLDFFKKIHSDDKQRLLLINQVKIY
SDGIIINGTASTLAPYDWSYTPELPRGLEYVPASKMTWWLTELNKIGYGAHIHAIGDAGVRNTLDAIETLRSQGATRPYA
MTHLEMVSPQDIKRFASLKVDADFQLGSDYIAAQDHEWAIPFIGKQRAHALLPINPIFKTGANVTLSSDWNVNDINPLVG
IANAVYLQNAGITDIHSAIAAYTINPANALGISDITGSIVLGKSADFVVLERDITQATAKQIADTQILMTWLEGELVFDL
NDQ

Sequences:

>Translated_563_residues
MKQTKTPHKLVKCLALTGLFTLSACANDELADTLLTNTHVYGHDQATSLAIKDGKIVYIGNSINAMDHVSNQTKVIDLKG
HYLLPGFIDNHNHVFEAASEIGSDCLLDDDTNLNGYIRTLTACKNQLETGEWLLGYGHQLSVVMQENGPTPLAILDKIFP
DNPVVIMEQTSHSMWVNSLALAKAKISAHTADPQGGKILKDEESGKLLGILFDNAGDLVMEQAWKSQPNLQQANYQGLLN
GLAAAAEHGITTIGDGRLYWKRDWLETWLAVESHDELTARVSLRPWIYPADEPQAQLDFFKKIHSDDKQRLLLINQVKIY
SDGIIINGTASTLAPYDWSYTPELPRGLEYVPASKMTWWLTELNKIGYGAHIHAIGDAGVRNTLDAIETLRSQGATRPYA
MTHLEMVSPQDIKRFASLKVDADFQLGSDYIAAQDHEWAIPFIGKQRAHALLPINPIFKTGANVTLSSDWNVNDINPLVG
IANAVYLQNAGITDIHSAIAAYTINPANALGISDITGSIVLGKSADFVVLERDITQATAKQIADTQILMTWLEGELVFDL
NDQ
>Mature_563_residues
MKQTKTPHKLVKCLALTGLFTLSACANDELADTLLTNTHVYGHDQATSLAIKDGKIVYIGNSINAMDHVSNQTKVIDLKG
HYLLPGFIDNHNHVFEAASEIGSDCLLDDDTNLNGYIRTLTACKNQLETGEWLLGYGHQLSVVMQENGPTPLAILDKIFP
DNPVVIMEQTSHSMWVNSLALAKAKISAHTADPQGGKILKDEESGKLLGILFDNAGDLVMEQAWKSQPNLQQANYQGLLN
GLAAAAEHGITTIGDGRLYWKRDWLETWLAVESHDELTARVSLRPWIYPADEPQAQLDFFKKIHSDDKQRLLLINQVKIY
SDGIIINGTASTLAPYDWSYTPELPRGLEYVPASKMTWWLTELNKIGYGAHIHAIGDAGVRNTLDAIETLRSQGATRPYA
MTHLEMVSPQDIKRFASLKVDADFQLGSDYIAAQDHEWAIPFIGKQRAHALLPINPIFKTGANVTLSSDWNVNDINPLVG
IANAVYLQNAGITDIHSAIAAYTINPANALGISDITGSIVLGKSADFVVLERDITQATAKQIADTQILMTWLEGELVFDL
NDQ

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 61941; Mature: 61941

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00087 SOD_CU_ZN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTKTPHKLVKCLALTGLFTLSACANDELADTLLTNTHVYGHDQATSLAIKDGKIVYIG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCEEEEEECCEEEEEC
NSINAMDHVSNQTKVIDLKGHYLLPGFIDNHNHVFEAASEIGSDCLLDDDTNLNGYIRTL
CCCCHHHHCCCCCEEEEECCCEECCCEECCCCHHHHHHHHCCCCCEECCCCCCCHHHHHH
TACKNQLETGEWLLGYGHQLSVVMQENGPTPLAILDKIFPDNPVVIMEQTSHSMWVNSLA
HHHHHHCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
LAKAKISAHTADPQGGKILKDEESGKLLGILFDNAGDLVMEQAWKSQPNLQQANYQGLLN
HHHHHHEEECCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHH
GLAAAAEHGITTIGDGRLYWKRDWLETWLAVESHDELTARVSLRPWIYPADEPQAQLDFF
HHHHHHHCCCEEEECCEEEEEHHHHHHHHHCCCCCCEEEEEEECCEEECCCCCHHHHHHH
KKIHSDDKQRLLLINQVKIYSDGIIINGTASTLAPYDWSYTPELPRGLEYVPASKMTWWL
HHHCCCCCCEEEEEEEEEEEECCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHH
TELNKIGYGAHIHAIGDAGVRNTLDAIETLRSQGATRPYAMTHLEMVSPQDIKRFASLKV
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHEEE
DADFQLGSDYIAAQDHEWAIPFIGKQRAHALLPINPIFKTGANVTLSSDWNVNDINPLVG
CCCCCCCCCEEEECCCCEECCCCCCCCCCEEECCCHHHHCCCCEEEECCCCCCCCCHHHH
IANAVYLQNAGITDIHSAIAAYTINPANALGISDITGSIVLGKSADFVVLERDITQATAK
HHHEEEEECCCCHHHHHHHHEEEECCHHCCCCCCCCCCEEECCCCCEEEEECHHHHHHHH
QIADTQILMTWLEGELVFDLNDQ
HHCCHHEEEEEECCEEEEEECCC
>Mature Secondary Structure
MKQTKTPHKLVKCLALTGLFTLSACANDELADTLLTNTHVYGHDQATSLAIKDGKIVYIG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCEEEEEECCEEEEEC
NSINAMDHVSNQTKVIDLKGHYLLPGFIDNHNHVFEAASEIGSDCLLDDDTNLNGYIRTL
CCCCHHHHCCCCCEEEEECCCEECCCEECCCCHHHHHHHHCCCCCEECCCCCCCHHHHHH
TACKNQLETGEWLLGYGHQLSVVMQENGPTPLAILDKIFPDNPVVIMEQTSHSMWVNSLA
HHHHHHCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHH
LAKAKISAHTADPQGGKILKDEESGKLLGILFDNAGDLVMEQAWKSQPNLQQANYQGLLN
HHHHHHEEECCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHH
GLAAAAEHGITTIGDGRLYWKRDWLETWLAVESHDELTARVSLRPWIYPADEPQAQLDFF
HHHHHHHCCCEEEECCEEEEEHHHHHHHHHCCCCCCEEEEEEECCEEECCCCCHHHHHHH
KKIHSDDKQRLLLINQVKIYSDGIIINGTASTLAPYDWSYTPELPRGLEYVPASKMTWWL
HHHCCCCCCEEEEEEEEEEEECCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHH
TELNKIGYGAHIHAIGDAGVRNTLDAIETLRSQGATRPYAMTHLEMVSPQDIKRFASLKV
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHEEE
DADFQLGSDYIAAQDHEWAIPFIGKQRAHALLPINPIFKTGANVTLSSDWNVNDINPLVG
CCCCCCCCCEEEECCCCEECCCCCCCCCCEEECCCHHHHCCCCEEEECCCCCCCCCHHHH
IANAVYLQNAGITDIHSAIAAYTINPANALGISDITGSIVLGKSADFVVLERDITQATAK
HHHEEEEECCCCHHHHHHHHEEEECCHHCCCCCCCCCCEEECCCCCEEEEECHHHHHHHH
QIADTQILMTWLEGELVFDLNDQ
HHCCHHEEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA