Click here to switch to the map view.

The map label for this gene is gap3 [H]

Identifier: 160877060

GI number: 160877060

Start: 4661385

End: 4662398

Strand: Reverse

Name: gap3 [H]

Synonym: Sbal195_3956

Alternate gene names: 160877060

Gene position: 4662398-4661385 (Counterclockwise)

Preceding gene: 160877061

Following gene: 160877059

Centisome position: 87.19

GC content: 51.48

Gene sequence:

>1014_bases
ATGGCAATCAAAATCGGCATTAATGGCTTTGGGCGTATGGGGCGTTTAGCACTGCGCGCGGCATGGGACTGGGATGAGGT
GGAGTTTGTGCAGATCAACGATCCCGCAGGTGATGCCCATACATTGGCACATTTACTCGAGTTTGATTCTGTCCATGGTC
GCTGGCGTTATCCCGTGACGGCGAATGCCGATGAGATCCAGATCCAAGATAAAATCATTCGCACCACGCGCAATAAAGTC
ATCGCCGATACCGATTGGTCCGGTTGCGATGTGGTGATTGAAGCCTCCGGCGTGATGAAAACCAAAGCGTTATTGCAAGC
TTATTTAGATCAAGGTGTTAAGCGGGTGGTTGTCACCGCTCCCGTGAAAGAAGAAGGCGTGCTAAACGTAGTGATCGGGG
TTAATCATCAGTTATATGACCCAGCCATGCACCCAATCGTCACTGCAGCCTCTTGCACCACTAATTGCCTTGCGCCTGTG
GTGAAAGTGATCCATGAGCAGATAGGCATCAAACACGGTTCTATGACCACTATCCATGATATTACTAACACCCAAACCAT
TCTCGATGCGCCGCATAAAGATTTACGCCGCGCCCGCGCCTGTGGTTTGAGTTTAATCCCAACCACCACTGGCAGCGCTA
CAGCAATTACTCATATATTCCCAGAGCTTAAAGGTAAACTTAATGGCCATGCCGTGCGGGTACCACTGGCTAATGCCTCG
CTGACGGATTGTGTATTCGAGCTTGAGCGCGCCGTTACCGAAGCGGAAGTGAATGCGCTACTTAAAACCGCCGCCGAGGG
TGAGCTTAAGGGGATTTTAGGCTATGAAGAGCGGCCGCTGGTGTCGGTGGATTACAAAACCGATCCGCGATCGAGCATTG
TTGATGCGCTCTCGACCATGGTGGTGAATGGCACTCAACTTAAGCTCTATGTGTGGTACGACAATGAATGGGGCTACGCT
AACCGTACGGCTGAGCTTGCCCGTTTAGTCGGCCAGCTTGATTTGCCGCACTAG

Upstream 100 bases:

>100_bases
AATGGCGATGTTATCGTTTAAGTAAACCCTTAGTAGCGTTAAGAAAATATAAAAAAATTAAAGACATAAAAATTTAAAAT
CAACATATTGGAATATCATC

Downstream 100 bases:

>100_bases
GAGAGCTCAATTGTTTGCCGCCAAGCCATTTGAACAACTGATGCAATTGCCTGCTGCCGTGCGGCAATATTTGTTGATCA
CCTTCAATTATTGGAGTTTT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAIKIGINGFGRMGRLALRAAWDWDEVEFVQINDPAGDAHTLAHLLEFDSVHGRWRYPVTANADEIQIQDKIIRTTRNKV
IADTDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNVVIGVNHQLYDPAMHPIVTAASCTTNCLAPV
VKVIHEQIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
LTDCVFELERAVTEAEVNALLKTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTMVVNGTQLKLYVWYDNEWGYA
NRTAELARLVGQLDLPH

Sequences:

>Translated_337_residues
MAIKIGINGFGRMGRLALRAAWDWDEVEFVQINDPAGDAHTLAHLLEFDSVHGRWRYPVTANADEIQIQDKIIRTTRNKV
IADTDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNVVIGVNHQLYDPAMHPIVTAASCTTNCLAPV
VKVIHEQIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
LTDCVFELERAVTEAEVNALLKTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTMVVNGTQLKLYVWYDNEWGYA
NRTAELARLVGQLDLPH
>Mature_336_residues
AIKIGINGFGRMGRLALRAAWDWDEVEFVQINDPAGDAHTLAHLLEFDSVHGRWRYPVTANADEIQIQDKIIRTTRNKVI
ADTDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNVVIGVNHQLYDPAMHPIVTAASCTTNCLAPVV
KVIHEQIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANASL
TDCVFELERAVTEAEVNALLKTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTMVVNGTQLKLYVWYDNEWGYAN
RTAELARLVGQLDLPH

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=327, Percent_Identity=41.8960244648318, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI7657116, Length=335, Percent_Identity=39.1044776119403, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=43.4650455927052, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1789295, Length=328, Percent_Identity=41.1585365853659, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17534677, Length=330, Percent_Identity=41.8181818181818, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17534679, Length=330, Percent_Identity=41.2121212121212, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32566163, Length=331, Percent_Identity=42.2960725075529, Blast_Score=263, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17568413, Length=331, Percent_Identity=42.2960725075529, Blast_Score=263, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=41.4414414414414, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321631, Length=333, Percent_Identity=41.1411411411411, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6322468, Length=333, Percent_Identity=40.5405405405405, Blast_Score=256, Evalue=3e-69,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=40.4191616766467, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=40.4191616766467, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=40.1197604790419, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=40.1197604790419, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19922412, Length=336, Percent_Identity=39.2857142857143, Blast_Score=247, Evalue=9e-66,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36913; Mature: 36782

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKIGINGFGRMGRLALRAAWDWDEVEFVQINDPAGDAHTLAHLLEFDSVHGRWRYPVT
CEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCEE
ANADEIQIQDKIIRTTRNKVIADTDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTA
CCCCCEEHHHHHHHHHCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNVVIGVNHQLYDPAMHPIVTAASCTTNCLAPVVKVIHEQIGIKHGSMTTIHD
CCCCCCEEEEEEECCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCH
LTDCVFELERAVTEAEVNALLKTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHH
VVNGTQLKLYVWYDNEWGYANRTAELARLVGQLDLPH
EECCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AIKIGINGFGRMGRLALRAAWDWDEVEFVQINDPAGDAHTLAHLLEFDSVHGRWRYPVT
EEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCEE
ANADEIQIQDKIIRTTRNKVIADTDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTA
CCCCCEEHHHHHHHHHCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNVVIGVNHQLYDPAMHPIVTAASCTTNCLAPVVKVIHEQIGIKHGSMTTIHD
CCCCCCEEEEEEECCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCH
LTDCVFELERAVTEAEVNALLKTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHH
VVNGTQLKLYVWYDNEWGYANRTAELARLVGQLDLPH
EECCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]