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The map label for this gene is amtB [C]

Identifier: 160876935

GI number: 160876935

Start: 4511700

End: 4512950

Strand: Reverse

Name: amtB [C]

Synonym: Sbal195_3831

Alternate gene names: 160876935

Gene position: 4512950-4511700 (Counterclockwise)

Preceding gene: 160876936

Following gene: 160876934

Centisome position: 84.4

GC content: 47.08

Gene sequence:

>1251_bases
ATGGAAGAATTAACAAAATTAGGCGGCACAGTCACTGAACTACGCTTTGCACTCGACACCTTTTACTTTTTGATTTCTGG
TGCGTTAGTTATGTGGATGGCGGCGGGATTTGCCATGTTAGAAGCGGGTTTAGTTCGCTCTAAAAACACCACTGAAATTT
TAACGAAGAACGTTGTGTTGTACGCGATTGCCTGTGTGATGTATCTGCTGGTTGGCTACAACATCATGTATGTGGATAAC
ACTGAAGGGGGTTGGTTGCCATCGATTGGTACCTTAATCGGTTCTCAATCATCAGATGCAAGCCATGCATTAGAATCTGA
CTTCTTCTTCCAAGTGGTGTTTGTGGCAACGGCGATGTCAATTGTCTCTGGCGCTGTGGCGGAACGGATGAAGTTATGGG
CTTTCCTGTTCTTCTCTGTGGTAATGACTGCGGTTATCTATCCAGTGGAAGGGTATTGGACTTGGGGCCAAGGTTTTATC
GCAAAACTGGGCTTTGTGGACTTTGCGGGTAGCGGTATTGTGCACATGACTGGTGCGACTGCAGCGATTGCGGGGGTATT
ATTGCTCGGTGCACGTAAGGGAAAATATGGCCCTAACGGTCAAGTGAATCCTATCCCAGGATCTAACTTGCCAATGGCAA
CCTTAGGTATGTTTATTCTGTGGATGGGCTGGTTTGGCTTCAACGGTGGCTCGCAATTAATGGTGTCGGATGCGGCGAAT
GCGAGCTCAGTGGCAAAAGTGTTCGTTAATACTAATACTGCGGCTGCATTTGGTGCTATCTCTGCGCTTATCGTATGTAA
GATGATTTGGGGTAAAGCGGATTTAACCATGATACTCAACGGTATTCTGGCGGGTTTAGTGGCGATTACGGCAGATCCGC
TATCACCTTCACTGTTAATGGCGGGCGTGATTGGTGTGGTTGCTGGTGGTTTAGTGGTGTTCTCTATTGTGGGCTTAGAT
CGCATTAAGATAGACGATCCAGTGGGCGCGATTTCAGTACACGGCGTGGCGGGTTTCCTTGGGTTAATGTGTGTGCCATT
ATCCAACGCTGATGCGACCATTACTGGGCAGTTGACCGGTGCAGCCTTCATCTTTACTTGGGTATTCTGTTCATCCTTTG
TGGTTTGGTATGCGATTAAGAAAACCATGGGGATCCGTGTGACTGAGGAAGAAGAATACAATGGTATGGATGCGTCTGAT
TGTGGTATCGATGCTTACCCAGAGTTTGTTACGGTTAAAAATGCTGGTTAA

Upstream 100 bases:

>100_bases
GGGAAAATTGGCGACGGTAAGATCTTCGTCGTCGACTTAGAACAAGCTATCCGTATCCGTACGGGTGAACTCGATACAGA
AGCGCTATAAGGGGCTGACA

Downstream 100 bases:

>100_bases
GTAAACTGGCGAATGAATGGTGATTCAACTTGATGCCTAGCGTGGATCGAGGGATAGTAAAGCAGAGCTTAGGCTCTGCT
TTTTTATGGGGCTTGGTCAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MEELTKLGGTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVLYAIACVMYLLVGYNIMYVDN
TEGGWLPSIGTLIGSQSSDASHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLFFSVVMTAVIYPVEGYWTWGQGFI
AKLGFVDFAGSGIVHMTGATAAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
ASSVAKVFVNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSLLMAGVIGVVAGGLVVFSIVGLD
RIKIDDPVGAISVHGVAGFLGLMCVPLSNADATITGQLTGAAFIFTWVFCSSFVVWYAIKKTMGIRVTEEEEYNGMDASD
CGIDAYPEFVTVKNAG

Sequences:

>Translated_416_residues
MEELTKLGGTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVLYAIACVMYLLVGYNIMYVDN
TEGGWLPSIGTLIGSQSSDASHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLFFSVVMTAVIYPVEGYWTWGQGFI
AKLGFVDFAGSGIVHMTGATAAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
ASSVAKVFVNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSLLMAGVIGVVAGGLVVFSIVGLD
RIKIDDPVGAISVHGVAGFLGLMCVPLSNADATITGQLTGAAFIFTWVFCSSFVVWYAIKKTMGIRVTEEEEYNGMDASD
CGIDAYPEFVTVKNAG
>Mature_416_residues
MEELTKLGGTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVLYAIACVMYLLVGYNIMYVDN
TEGGWLPSIGTLIGSQSSDASHALESDFFFQVVFVATAMSIVSGAVAERMKLWAFLFFSVVMTAVIYPVEGYWTWGQGFI
AKLGFVDFAGSGIVHMTGATAAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
ASSVAKVFVNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSLLMAGVIGVVAGGLVVFSIVGLD
RIKIDDPVGAISVHGVAGFLGLMCVPLSNADATITGQLTGAAFIFTWVFCSSFVVWYAIKKTMGIRVTEEEEYNGMDASD
CGIDAYPEFVTVKNAG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=410, Percent_Identity=30.7317073170732, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17550180, Length=452, Percent_Identity=34.5132743362832, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17550182, Length=429, Percent_Identity=34.7319347319347, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=27.790973871734, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=422, Percent_Identity=27.0142180094787, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6325396, Length=422, Percent_Identity=28.6729857819905, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=413, Percent_Identity=28.0871670702179, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=444, Percent_Identity=27.9279279279279, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=34.6341463414634, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43939; Mature: 43939

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELTKLGGTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YAIACVMYLLVGYNIMYVDNTEGGWLPSIGTLIGSQSSDASHALESDFFFQVVFVATAMS
HHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLFFSVVMTAVIYPVEGYWTWGQGFIAKLGFVDFAGSGIVHMTGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCEECCCCEEEECCHH
AAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ASSVAKVFVNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSLLM
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGVIGVVAGGLVVFSIVGLDRIKIDDPVGAISVHGVAGFLGLMCVPLSNADATITGQLTG
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHEEECCCCCEEEEEECCH
AAFIFTWVFCSSFVVWYAIKKTMGIRVTEEEEYNGMDASDCGIDAYPEFVTVKNAG
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MEELTKLGGTVTELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVVL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YAIACVMYLLVGYNIMYVDNTEGGWLPSIGTLIGSQSSDASHALESDFFFQVVFVATAMS
HHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLFFSVVMTAVIYPVEGYWTWGQGFIAKLGFVDFAGSGIVHMTGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCEECCCCEEEECCHH
AAIAGVLLLGARKGKYGPNGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ASSVAKVFVNTNTAAAFGAISALIVCKMIWGKADLTMILNGILAGLVAITADPLSPSLLM
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGVIGVVAGGLVVFSIVGLDRIKIDDPVGAISVHGVAGFLGLMCVPLSNADATITGQLTG
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHEEECCCCCEEEEEECCH
AAFIFTWVFCSSFVVWYAIKKTMGIRVTEEEEYNGMDASDCGIDAYPEFVTVKNAG
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]