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The map label for this gene is mpl [H]

Identifier: 160876819

GI number: 160876819

Start: 4382655

End: 4384046

Strand: Reverse

Name: mpl [H]

Synonym: Sbal195_3715

Alternate gene names: 160876819

Gene position: 4384046-4382655 (Counterclockwise)

Preceding gene: 160876820

Following gene: 160876818

Centisome position: 81.99

GC content: 48.42

Gene sequence:

>1392_bases
ATGCACGTACATATTTTAGGAATTTGTGGAACCTTCATGGGTGGTCTAGCGTTATTGGCTAGGGCAAAAGGGCACAGAGT
GACGGGCTCGGATGCCAATGTCTATCCTCCAATGAGCACGCAACTAGAGGACCAAGGCATTGAGCTTATTCAAGGTTTTG
ATCCCAGCCAGCTGGGTGAAGCAGGGCATTATCCTGACTTAGTTGTCATTGGTAATGCCATGAGCCGGGGTAATCCTTGC
GTGGAAGCCGTGTTGAACATTGGCATCCCTTATACCTCTGGCCCGCAGTTTTTAGCCGATCATATTTTGCCTGAGCGTTG
GGTGCTGGCAGTGTCTGGCACTCACGGTAAAACCTCGACTTCTAGCATGTTAGCCTGGATTTTAGATGATTGCGGTTATG
AGCCTGGATTTTTAATCGGTGGCGTACCGCAAAACTTTGGTGTGTCGGCGCGTTTAGGCAAGACCGCTTTCTTTGTGGTT
GAGGCCGACGAGTACGACAGTGCCTTCTTCGATAAACGTTCGAAATTCGTGCATTATCATCCACGTACCTTAGTGATCAA
CAATCTTGAGTTCGATCATGCTGATATTTTTGCCGATCTGGCCGCGATTCAAAAACAGTTTAATCATTTAGTTCGCACTG
TACCTGGCGAAGGAAAGATCGTTTGGCCCGCCGATTCCCATGCCGTTAAGCAAACGATTGATATGGGTTGCTGGAGCGAG
CAAGAAACCTATCATTTAGCCGAATCGACCAGCGGTTGGTATGGCAAAGCCTTAAGTGATGATTGCCATCGGTTTGAAGT
GTTTTTTGATGGCGAGTCCCAAGGCATTCTCGAATGGGATTTGATTGGTCAGCACAATATTGAAAACGCCATGATGGCCA
TTGCTGCCGCGCGTCACGTGGGCGTTAAGCCGAGCGCTGCAATTGAAGCTTTAGCTAAGTTTATGCCACCTAAACGTCGA
CTCGAATTACTCGGAACCGTTAACGGCGTGAGCGTTTACGACGACTTTGCCCACCATCCAACTGCGATTGCGACCACGTT
AAAAGGCATGCGGGCTAAGGTTGGTCAGAGCAAAATTACTGTGGTACTCGAGCCGCGTTCAAATACCATGAAGAGCGGTG
TCCATAAAGACACTTTAGCTTTTTCTATGATGCAAGCCGACGAAGCTTTTTTATATCAAGCCGATACCATTGACTGGAAT
ATGAAAGAAGCCATGGAAGCGGCGGTGATCCCTGTGACTGTGTTACATCAAATTGATGATGTGGTCGCCAAAGTAACGGC
AACGGCCAAAGCGGGCGACACCATAGTGGTGATGAGTAATGGCGGTTTTGGTGGGTTACATAAGAAGCTGCTGGCCGCAT
TAGAGCAGCGTTCAGCTGTAGAGCAGGTTTAA

Upstream 100 bases:

>100_bases
TGTCATTTGCGATTGCAGTGCTTTTCGCCGATTGTTACCTGCCAATCTGTCGCCCGATGGATTAGAATGGCATTGAATTC
AAAACATCAGAGCGAGCGGT

Downstream 100 bases:

>100_bases
GATGAGCTACACAAAGTCGACAAAGGCAATTAGCTTAGCTTGGACAGGCGCCTCGGGCGCCCCCTATGGCCTTAAGTTGC
TCGAGTGTCTGTTGGTATCG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGEAGHYPDLVVIGNAMSRGNPC
VEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
QETYHLAESTSGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHVGVKPSAAIEALAKFMPPKRR
LELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKITVVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALEQRSAVEQV

Sequences:

>Translated_463_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGEAGHYPDLVVIGNAMSRGNPC
VEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
QETYHLAESTSGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHVGVKPSAAIEALAKFMPPKRR
LELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKITVVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALEQRSAVEQV
>Mature_463_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGEAGHYPDLVVIGNAMSRGNPC
VEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
QETYHLAESTSGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHVGVKPSAAIEALAKFMPPKRR
LELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKITVVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALEQRSAVEQV

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=463, Percent_Identity=62.6349892008639, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1786279, Length=384, Percent_Identity=27.34375, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50373; Mature: 50373

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGE
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCC
AGHYPDLVVIGNAMSRGNPCVEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTST
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCH
SSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVVEADEYDSAFFDKRSKFVHYH
HHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEECCCCCHHHHHCCCCEEEEC
PRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
QETYHLAESTSGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHV
CCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GVKPSAAIEALAKFMPPKRRLELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKIT
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWNMKEAMEAAVIPVTVLHQIDD
EEECCCCCHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALEQRSAVEQV
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGE
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCC
AGHYPDLVVIGNAMSRGNPCVEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTST
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCH
SSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVVEADEYDSAFFDKRSKFVHYH
HHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEECCCCCHHHHHCCCCEEEEC
PRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
QETYHLAESTSGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHV
CCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GVKPSAAIEALAKFMPPKRRLELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKIT
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWNMKEAMEAAVIPVTVLHQIDD
EEECCCCCHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALEQRSAVEQV
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]