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The map label for this gene is mnmC [H]

Identifier: 160875951

GI number: 160875951

Start: 3383654

End: 3385780

Strand: Direct

Name: mnmC [H]

Synonym: Sbal195_2841

Alternate gene names: 160875951

Gene position: 3383654-3385780 (Clockwise)

Preceding gene: 160875941

Following gene: 160875958

Centisome position: 63.28

GC content: 49.22

Gene sequence:

>2127_bases
TTGACTGCAGAGCCCAATAAACCTTGCCAAATTAAGCGTGATTACCAGCAATTAATCAACCTTTATCCCGCAACAGCTCA
CACTGATGCACACTATCTCAGCAAGTTGAGCATTTATCAGCAGCGGGTATTTGAGGCTCATTCACAGCAAAAACTCTTAA
TATTAGGCCAAATTGGTTTAGGTAACGGGCTTGAACTCTTATCTTGGTGGCGCACTCAGGCGAACCCTAGCCAACGTTTA
CTCCTTAAGGTGTTCGAACCCAATCCGATTAATGCGTACGAACTTAAATCACTTTGGGATCAATCACTCACTCTTGTGAA
AGAAACCCCATTCGAGCCACATCTTGAATCAAAGCAAGCGTCAGCGTTGGAGTGTAAATCTAAGCTCAGCCAGCTTGCTC
AAACCTTACTCGATGCTGAGCCGACGGCTATTATAGGTTGCCAGAGATTAATTTTTGATGACGGTCGTACCACTATCGAC
CTGCACTTTGGTGATATTCAAACCCAGCTCAGTTCACTCACCCATTCGCCAATGCATCCGGTTCAACACTGGTTGATATT
GCCCCATCTGCTACAGGCGCTATATCATCAAAGCCAATGGCAGATGGCAAAACTCAGTGACGATAGCGCAACCATTGCCA
CGATTGGATTAAGCGAATCAAGCGAATTAAGCGAAACAACAGTCAACCGTTTCCAAGCCTGTGGCTTTACTGTGAGCGAT
ATCAATGAACTTGGTACCAAAGCTCTGGGTATCCATCAACCAGTCACACTGATTAACCACCAGCCTGACGCCGTACTGCT
ACACGAGCGCCAAGTCTTACGTCAGCAAGACGCTAAAGCCTATGCTTTTAATCCTATGGCTGCAATCTTATCCTCGCCGA
CGCAAAGCTCGATTGCCATTATAGGCGGCGGCTTAGCCTCGGCGCATTTGACGCTGTCTTTGGCTGAGCGCGGCCAAGGT
GCACAGGTATTTTGTAAAGATGCCGAGCTAGGCCAAGGCGCATCGGGCAATCGCCAAGGAGCCATTTATCCACTGCTGAC
AACGGAAAATGATGAATTAAGCCGCTTCTTCCAACAGGCATTTTTATTTAGCCGACGTCGAGTGCAGGCGCTGACATCCG
CCCCCGCCGACAATCAGACGCCGATTTCCCATAATTTTTGCGGCGTATTGCAAACTGCCCACGATGAACGCAGCCAGTTA
CGACTCGATAAAATCATTCAAGGCCAACCCTGGCCAAGTGAAATCGCCTACGCCGTCGATGCTGAGCAAGCCAATGCAAT
TGCCAAGATCAATCTCGATAAACCAGGTTTTTTTTATCCCTTAGGTGGCTGGGTTTGCCCATTTGAATATGCCGACGCTG
CGATACAAAAAGCGATGCAACTGGCCGATGTCAGCGTCTCGCTCAATACTGACATCCTTGCCATTGAACGCCAAGCCGAT
GGCTGGGTTTTGTTGACTGAAAAGGAACGTTTCGGGCCCTTTGCACAACTTGTACTCGCCAATGGCGCTGAATTGACACA
ATTTGATGCCAGTAACAAATTACAAATTAGCCCCTTTCGCGGTCAAGTCAGCCATGTGCCCGCTCAATTTCAATTGAGTC
AGCTCGCAACTGTGCTGTGCGCCAACGGTTATTTAACGCCAAGTCACCAAGGACTCCACTGCCTTGGCGCGAGTTACGTT
AAAGAACCCGAGCATTTAGACTTTTGCTCGCAGGAACAACAAGAAAACCTCATGAAAATGCACGAGAGTTATCCGAACCA
AAGCTGGCTTGAAGATATCGACATGAGTGGCAACAATGCCCGCATCGGCGTACGTATGGTGACGCGGGATCACTTCCCTA
TGATGGGCTGCGCGCCCGATGTGGCGAAAATCCTTGAGGATTATGAGCAACATCAACTCACTAAAGAAAGTCGCCACTAC
TGGCAAACCACACCAGCGCCGGTGCATCAAGGACTGTATATTCTTGGTGGGTTAGGCTCACGGGGACTCAGTTCTGGGCC
ACTGGCCGCCGAATGTCTCGCGGCGCAGCTTTGCGGCGAACCAATACCGCTCGATAAAGAAACCCTGTGTAAACTCAACC
CCAATCGCATGTGGCTGCGAAAATTGCTCAAGGGTAAAGCGCTCTAG

Upstream 100 bases:

>100_bases
TTCGCTGAGCCAATAGTATCTGTTTTGACTCAATTAAGTGGTCAGCTTTCCATAAAGGCGAGTAAAATAATGCCAATTAT
GAGACCGAGAGTAAATTTCT

Downstream 100 bases:

>100_bases
GTAATCAATCGCTAGAAAAATAACGTTTTTGAGCGCTATAAAAAAGGGACTAAGTCTCTGCTTAATCCCTTTTTTAAATG
TTTTCTGTAAACCACTTAAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MTAEPNKPCQIKRDYQQLINLYPATAHTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGLGNGLELLSWWRTQANPSQRL
LLKVFEPNPINAYELKSLWDQSLTLVKETPFEPHLESKQASALECKSKLSQLAQTLLDAEPTAIIGCQRLIFDDGRTTID
LHFGDIQTQLSSLTHSPMHPVQHWLILPHLLQALYHQSQWQMAKLSDDSATIATIGLSESSELSETTVNRFQACGFTVSD
INELGTKALGIHQPVTLINHQPDAVLLHERQVLRQQDAKAYAFNPMAAILSSPTQSSIAIIGGGLASAHLTLSLAERGQG
AQVFCKDAELGQGASGNRQGAIYPLLTTENDELSRFFQQAFLFSRRRVQALTSAPADNQTPISHNFCGVLQTAHDERSQL
RLDKIIQGQPWPSEIAYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAIERQAD
GWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLCANGYLTPSHQGLHCLGASYV
KEPEHLDFCSQEQQENLMKMHESYPNQSWLEDIDMSGNNARIGVRMVTRDHFPMMGCAPDVAKILEDYEQHQLTKESRHY
WQTTPAPVHQGLYILGGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL

Sequences:

>Translated_708_residues
MTAEPNKPCQIKRDYQQLINLYPATAHTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGLGNGLELLSWWRTQANPSQRL
LLKVFEPNPINAYELKSLWDQSLTLVKETPFEPHLESKQASALECKSKLSQLAQTLLDAEPTAIIGCQRLIFDDGRTTID
LHFGDIQTQLSSLTHSPMHPVQHWLILPHLLQALYHQSQWQMAKLSDDSATIATIGLSESSELSETTVNRFQACGFTVSD
INELGTKALGIHQPVTLINHQPDAVLLHERQVLRQQDAKAYAFNPMAAILSSPTQSSIAIIGGGLASAHLTLSLAERGQG
AQVFCKDAELGQGASGNRQGAIYPLLTTENDELSRFFQQAFLFSRRRVQALTSAPADNQTPISHNFCGVLQTAHDERSQL
RLDKIIQGQPWPSEIAYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAIERQAD
GWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLCANGYLTPSHQGLHCLGASYV
KEPEHLDFCSQEQQENLMKMHESYPNQSWLEDIDMSGNNARIGVRMVTRDHFPMMGCAPDVAKILEDYEQHQLTKESRHY
WQTTPAPVHQGLYILGGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL
>Mature_707_residues
TAEPNKPCQIKRDYQQLINLYPATAHTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGLGNGLELLSWWRTQANPSQRLL
LKVFEPNPINAYELKSLWDQSLTLVKETPFEPHLESKQASALECKSKLSQLAQTLLDAEPTAIIGCQRLIFDDGRTTIDL
HFGDIQTQLSSLTHSPMHPVQHWLILPHLLQALYHQSQWQMAKLSDDSATIATIGLSESSELSETTVNRFQACGFTVSDI
NELGTKALGIHQPVTLINHQPDAVLLHERQVLRQQDAKAYAFNPMAAILSSPTQSSIAIIGGGLASAHLTLSLAERGQGA
QVFCKDAELGQGASGNRQGAIYPLLTTENDELSRFFQQAFLFSRRRVQALTSAPADNQTPISHNFCGVLQTAHDERSQLR
LDKIIQGQPWPSEIAYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAIERQADG
WVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLCANGYLTPSHQGLHCLGASYVK
EPEHLDFCSQEQQENLMKMHESYPNQSWLEDIDMSGNNARIGVRMVTRDHFPMMGCAPDVAKILEDYEQHQLTKESRHYW
QTTPAPVHQGLYILGGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=675, Percent_Identity=33.9259259259259, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 78545; Mature: 78414

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPNKPCQIKRDYQQLINLYPATAHTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGL
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GNGLELLSWWRTQANPSQRLLLKVFEPNPINAYELKSLWDQSLTLVKETPFEPHLESKQA
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
SALECKSKLSQLAQTLLDAEPTAIIGCQRLIFDDGRTTIDLHFGDIQTQLSSLTHSPMHP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCEEEEEEECHHHHHHHHHCCCCCHH
VQHWLILPHLLQALYHQSQWQMAKLSDDSATIATIGLSESSELSETTVNRFQACGFTVSD
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
INELGTKALGIHQPVTLINHQPDAVLLHERQVLRQQDAKAYAFNPMAAILSSPTQSSIAI
HHHHCCHHCCCCCCCCEECCCCCEEEHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCEEE
IGGGLASAHLTLSLAERGQGAQVFCKDAELGQGASGNRQGAIYPLLTTENDELSRFFQQA
EECCCCCCEEEEEEHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
FLFSRRRVQALTSAPADNQTPISHNFCGVLQTAHDERSQLRLDKIIQGQPWPSEIAYAVD
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEE
AEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAIERQAD
HHHCCEEEEEECCCCCEEEECCCEECCHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCC
GWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLC
CEEEEECCHHCCHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHCCCHHHHHHHHHHHHH
ANGYLTPSHQGLHCLGASYVKEPEHLDFCSQEQQENLMKMHESYPNQSWLEDIDMSGNNA
CCCCCCCCCCCCHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
RIGVRMVTRDHFPMMGCAPDVAKILEDYEQHQLTKESRHYWQTTPAPVHQGLYILGGLGS
EEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEECCCC
RGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHEECCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TAEPNKPCQIKRDYQQLINLYPATAHTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGL
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GNGLELLSWWRTQANPSQRLLLKVFEPNPINAYELKSLWDQSLTLVKETPFEPHLESKQA
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
SALECKSKLSQLAQTLLDAEPTAIIGCQRLIFDDGRTTIDLHFGDIQTQLSSLTHSPMHP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCEEEEEEECHHHHHHHHHCCCCCHH
VQHWLILPHLLQALYHQSQWQMAKLSDDSATIATIGLSESSELSETTVNRFQACGFTVSD
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
INELGTKALGIHQPVTLINHQPDAVLLHERQVLRQQDAKAYAFNPMAAILSSPTQSSIAI
HHHHCCHHCCCCCCCCEECCCCCEEEHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCEEE
IGGGLASAHLTLSLAERGQGAQVFCKDAELGQGASGNRQGAIYPLLTTENDELSRFFQQA
EECCCCCCEEEEEEHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
FLFSRRRVQALTSAPADNQTPISHNFCGVLQTAHDERSQLRLDKIIQGQPWPSEIAYAVD
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEE
AEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAIERQAD
HHHCCEEEEEECCCCCEEEECCCEECCHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCC
GWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLC
CEEEEECCHHCCHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHCCCHHHHHHHHHHHHH
ANGYLTPSHQGLHCLGASYVKEPEHLDFCSQEQQENLMKMHESYPNQSWLEDIDMSGNNA
CCCCCCCCCCCCHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
RIGVRMVTRDHFPMMGCAPDVAKILEDYEQHQLTKESRHYWQTTPAPVHQGLYILGGLGS
EEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEECCCC
RGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA