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The map label for this gene is rlmI [H]

Identifier: 160875888

GI number: 160875888

Start: 3307536

End: 3308453

Strand: Reverse

Name: rlmI [H]

Synonym: Sbal195_2777

Alternate gene names: 160875888

Gene position: 3308453-3307536 (Counterclockwise)

Preceding gene: 160875889

Following gene: 160875886

Centisome position: 61.87

GC content: 49.46

Gene sequence:

>918_bases
ATGCAAAGTTTTATACAAGCGCTAGCGACAATTGAATTAGGTGATGATGCAGTGCGGTTATTCCATGGCCGCGGTGGACA
CTTCGCGGGTTGTGAACACCTATGTTTAGATTGGTTTGCGCCAGTGCTATTACTGACCAGTTTCACTCCCTTAGCCGATG
ACGATTTAGCCACATGCCAGCAGGCCATTGCGGCGCGTTGGCAGGCCATTCGTCCTGATAGCCAACTGAATTTAGTCTAC
CAATACCGCAGTGCGGGCGAGACCTTTACTCAAGTGCTGCAAGGCGAAGTGCCTGAGCCGCATATCGTTAGTGAAAATGG
CGCACGCTTTCAGGTGCATTTAATGCGCGGACAAAACCACGGTTTATTCCTCGATATGGCTAACGGCCGCGAGTGGGTGA
GAGCAAATGCCCGCTATAAAAAAGTGCTTAATTTGTTTGCCTATACCTGTGGTTTCTCGGTGGCGGCATTGCAAGGCGGC
GCCGATGAAGTGGTCAATATGGACATGAGCAAAGGCGCATTAGGCATAGGTAAACAGAATCATTTGCTGAATGGCTTTAG
CGCAGGGGCACGTTTTTTAGGCCATGATATTTTTAAATCTTGGGGCAAACTGAAAAAGCTTGGTCCCTACGACATTATTG
TGGCCGATCCACCGAGCAATCAGAAGGGCAGTTTTGTCGCAACTAAGGATTATGTGCGTTTAATTAGGCGTTTACCTGAG
CTGCTGGCCGAGAATGGCGAAGTACTACTTTGTCTCAATGCACCCGAGCTTGATACCACTTTCTTACGCCAGCAGGTCGC
AGATGCGGCGCCAGCACTCGAATACATAGAGCGCTTAGCCAATCCGCCAGCGTTTATCGATGTGTCTGAAGAGAAGTCGC
TTAAGGTGCTGCGTTATCGTTTATCGACTCAAGCCTAG

Upstream 100 bases:

>100_bases
GGACGATGTTGAAGATAAGTTCGTGGCCAACAACACGGAAAACTCAAGTTCCGATGCCGATGAAGCCGATACAAGTACAA
CAGAGAGTAAGTAATAGAAG

Downstream 100 bases:

>100_bases
TCTAGATTTAGCATAACAGGCCAGTATTAACTGGCCTTTTTTGTCGGTTTTTAATGAAGCTAAATGGAAAATTTAATTGC
TGATTTTAAAAGTGCAACAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MQSFIQALATIELGDDAVRLFHGRGGHFAGCEHLCLDWFAPVLLLTSFTPLADDDLATCQQAIAARWQAIRPDSQLNLVY
QYRSAGETFTQVLQGEVPEPHIVSENGARFQVHLMRGQNHGLFLDMANGREWVRANARYKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALGIGKQNHLLNGFSAGARFLGHDIFKSWGKLKKLGPYDIIVADPPSNQKGSFVATKDYVRLIRRLPE
LLAENGEVLLCLNAPELDTTFLRQQVADAAPALEYIERLANPPAFIDVSEEKSLKVLRYRLSTQA

Sequences:

>Translated_305_residues
MQSFIQALATIELGDDAVRLFHGRGGHFAGCEHLCLDWFAPVLLLTSFTPLADDDLATCQQAIAARWQAIRPDSQLNLVY
QYRSAGETFTQVLQGEVPEPHIVSENGARFQVHLMRGQNHGLFLDMANGREWVRANARYKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALGIGKQNHLLNGFSAGARFLGHDIFKSWGKLKKLGPYDIIVADPPSNQKGSFVATKDYVRLIRRLPE
LLAENGEVLLCLNAPELDTTFLRQQVADAAPALEYIERLANPPAFIDVSEEKSLKVLRYRLSTQA
>Mature_305_residues
MQSFIQALATIELGDDAVRLFHGRGGHFAGCEHLCLDWFAPVLLLTSFTPLADDDLATCQQAIAARWQAIRPDSQLNLVY
QYRSAGETFTQVLQGEVPEPHIVSENGARFQVHLMRGQNHGLFLDMANGREWVRANARYKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALGIGKQNHLLNGFSAGARFLGHDIFKSWGKLKKLGPYDIIVADPPSNQKGSFVATKDYVRLIRRLPE
LLAENGEVLLCLNAPELDTTFLRQQVADAAPALEYIERLANPPAFIDVSEEKSLKVLRYRLSTQA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=264, Percent_Identity=30.3030303030303, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787181, Length=186, Percent_Identity=32.258064516129, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 33760; Mature: 33760

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSFIQALATIELGDDAVRLFHGRGGHFAGCEHLCLDWFAPVLLLTSFTPLADDDLATCQ
CHHHHHHHHHHCCCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
QAIAARWQAIRPDSQLNLVYQYRSAGETFTQVLQGEVPEPHIVSENGARFQVHLMRGQNH
HHHHHHHHHCCCCCCEEEEEEEHHCCHHHHHHHCCCCCCCCEECCCCCEEEEEEEECCCC
GLFLDMANGREWVRANARYKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALGIGKQN
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHCCCCCCCCCCCCCCC
HLLNGFSAGARFLGHDIFKSWGKLKKLGPYDIIVADPPSNQKGSFVATKDYVRLIRRLPE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLAENGEVLLCLNAPELDTTFLRQQVADAAPALEYIERLANPPAFIDVSEEKSLKVLRYR
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
LSTQA
HHCCC
>Mature Secondary Structure
MQSFIQALATIELGDDAVRLFHGRGGHFAGCEHLCLDWFAPVLLLTSFTPLADDDLATCQ
CHHHHHHHHHHCCCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
QAIAARWQAIRPDSQLNLVYQYRSAGETFTQVLQGEVPEPHIVSENGARFQVHLMRGQNH
HHHHHHHHHCCCCCCEEEEEEEHHCCHHHHHHHCCCCCCCCEECCCCCEEEEEEEECCCC
GLFLDMANGREWVRANARYKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALGIGKQN
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHCCCCCCCCCCCCCCC
HLLNGFSAGARFLGHDIFKSWGKLKKLGPYDIIVADPPSNQKGSFVATKDYVRLIRRLPE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLAENGEVLLCLNAPELDTTFLRQQVADAAPALEYIERLANPPAFIDVSEEKSLKVLRYR
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
LSTQA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA