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The map label for this gene is focA [H]

Identifier: 160875879

GI number: 160875879

Start: 3296079

End: 3297071

Strand: Reverse

Name: focA [H]

Synonym: Sbal195_2768

Alternate gene names: 160875879

Gene position: 3297071-3296079 (Counterclockwise)

Preceding gene: 160875882

Following gene: 160875874

Centisome position: 61.66

GC content: 48.34

Gene sequence:

>993_bases
ATGAAAAGCCCAAATCCATCATCAACCCCAGCGGTGACAAACACTGCCCACGAAGCGCCACAGGTTTCAGCGCACTTTAG
TTGTTACAATCAGGCGGAAATTTACGGTACCAGCAAAGTGGTTAAAGCGCCTTGGCAATCCTTTGGCCTGGCTGTGTTTG
CTGGCGCCTTCATTGCCTTAGCCTTTGTATTTTATCTGACAGTGACCACAGGTGCTGGCGATGCGTCTTGGGGCTTAGTT
CGCTTCGTTGGTGGGATAGCCTTTAGTTTAGGTCTGATTTTAGTGGTCGTTTGCGGCGGAGAACTGTTCACCAGCTCAGT
ACTGAGTACCGTCGCCTGGGCACAAAAACAAGTCTCTGGCGCTGAATTACTCAAGTGTTGGTGCCGGGTTTATGCGGGCA
ATTTTGTCGGCGCTATACTGATGGTTTGTTTGATCACCGCCGCAGGCATGTATGAACTCGATGGTGGTAACTGGGGATTA
AATGCCCTTAAAGTGGCGCAACACAAACTGCATCACACTTGGATCCAAGCTTTTAGCTTAGGCATTTTGTGTAACATGTT
GGTGTGTTTAGGCGTGTGGATGACCTTCGCCAGCCGTGATGTGTTGACTAAATCCCTACTGTTGATCTTACCCGTGGCCA
TGTTCGTCAGCAGCGGTTTTGAACACAGTATCGCTAACCTGTTTATGGTGCCTATGGGCATGATGATCCAATCTATCGCC
AGCCCAGAGTTCTTTGCATCTTTAGGTGTGACTCAAGAGCAGTTTGCCGATTTAACTGTAACTAACTTTATATTCAACAA
CTTAATCCCAGTCACCCTTGGCAATATTGTCGGCGGCGGTGTGATTGTGGGTTTAGGTTATTGGTGGATAGAGCAGGGGA
AAACGACGTCAAGCGATCCACACAAAGCTCACCATGAGCACTTTTCAGCGCCCCATTCACATACCGAAAAACCTCAACAT
GCGAGTGAAATCAAGCTGACGAAGCATTCCTAG

Upstream 100 bases:

>100_bases
TCAACATCTCGGTTTTGATTCGTGCTATCTGCGCAACGGCGCTGCAGGAGAGATATATGCTACGCATTCAAGTCTGTTTA
TCTGAAGTTGGAGTCATGCA

Downstream 100 bases:

>100_bases
AATGTTTTAAATAACCCGTTGAAGGATAATAAAATACCCAGCAAATAGCTGGGCATTTTTATGCCTATTCACCGGGCTTC
GTAAATGAAGAAAATAAAAA

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKSPNPSSTPAVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDASWGLV
RFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSGAELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGL
NALKVAQHKLHHTWIQAFSLGILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTTSSDPHKAHHEHFSAPHSHTEKPQH
ASEIKLTKHS

Sequences:

>Translated_330_residues
MKSPNPSSTPAVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDASWGLV
RFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSGAELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGL
NALKVAQHKLHHTWIQAFSLGILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTTSSDPHKAHHEHFSAPHSHTEKPQH
ASEIKLTKHS
>Mature_330_residues
MKSPNPSSTPAVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDASWGLV
RFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSGAELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGL
NALKVAQHKLHHTWIQAFSLGILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTTSSDPHKAHHEHFSAPHSHTEKPQH
ASEIKLTKHS

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=259, Percent_Identity=55.5984555984556, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1788837, Length=256, Percent_Identity=40.625, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI87082249, Length=247, Percent_Identity=28.3400809716599, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321779, Length=206, Percent_Identity=28.1553398058252, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 35513; Mature: 35513

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00589 PTS_HPR_SER ; PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPNPSSTPAVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIAL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHHH
AFVFYLTVTTGAGDASWGLVRFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCH
AELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGLNALKVAQHKLHHTWIQAFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
GILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTTSSDP
CHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
HKAHHEHFSAPHSHTEKPQHASEIKLTKHS
CHHHHHHCCCCCCCCCCCCCHHHCEEECCC
>Mature Secondary Structure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CHHHHHHCCCCCCCCCCCCCHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]