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The map label for this gene is cpdB [H]

Identifier: 160875855

GI number: 160875855

Start: 3255911

End: 3257956

Strand: Direct

Name: cpdB [H]

Synonym: Sbal195_2744

Alternate gene names: 160875855

Gene position: 3255911-3257956 (Clockwise)

Preceding gene: 160875853

Following gene: 160875856

Centisome position: 60.89

GC content: 45.89

Gene sequence:

>2046_bases
ATGTTAAATAAGAAAATACTCGCAGTGATGATCACTGCCGCCCTTGGTGTCAGCGCCTGTAATTCTGATGACGACAAAGT
CGTTCAAGCCGATTTACGGGTACTCGAAACCACAGATCTGCACACAAATATCATGGATTTCAACTACTACAGTGGTAAAG
ACGATCCCACAATCGGTCTTGCCCGCACTGCAAGCTTGATCCACGCTGCACGCAACGAAGCCAAGAATAGCGTCCTCGTT
GATAACGGCGATCTACTGCAAGGCAGCCCTATGGGCGATTATATGGCTAAAGTGGGCCTGCAAATGGGCGATGTTCACCC
TGCTTATAAAGCCATGAACCTATTAGATTATGAAGTCGGCAATATCGGTAATCATGAATTTAACTATGGTTTAGATTTCT
TAAATAAATCCATTAGTGGTGCAGATTTCCCTTATATCAACTCGAACGTTTATTGCGCCGACGATAATGGTTGCTGGAAA
GAAGTTAAAAAAGGTGAGCATTTATTCACCCCTTATATCATCAAACAAAAAATCATTATCGACAGCGAAGGCAATAATCA
AACCATTAACATTGGTTATATTGGTTTTGTGCCACCGCAAATTCTATTGTGGGATAAACAAAATCTGACGGGAAAAGTGA
CGGTTGAAGATATCGTTACCACCGCGAAAAAATATGTCCCAGAAATGAAAGCTAAAGGGGCCGATATCATTATCGCCATT
CCACACTCTGGCATTGGTTCGACGGAAAATCCTGGCGATCCTATGGCTGAAAACGCCACTTACGCTTTGACTTATGTTGA
CGGTATCGATGCGATTCTATTCGGCCACAGCCACTCTATCTTCCCAGATGCTAAGTATGCTGACTTACCCAATACCGATG
TCACTAAGGGCCTACTGAACGGCGTTCCCGCAGTGATGCCAGGTCGTTGGGGTGATAACTTAGGGGTGGTTGACTTTAAA
CTCGAACGTAGAGATGACAAGTGGCATGTCCTTACTGCCAAAACGCAAGCTCGCCCTATCTATGATGGCGCAAATAAAGC
CCCATTAGTTGAAGCGGATGCTGCTATTCACGATGCTGTCGCAGCCGAGCACCAAGGTACCCTCGCCTTTGTGGATGAAC
CTATCGGTGTAGCCGCGGCGGACATGTATAGCTTCCTTACCTTAGTGCAAGACGACCCTACGGTACAAATCGTTTCCGAT
GCGCAAATCGCTAACGTGAAAGCGAAATTACCTGCAGCATTAAAAGGGTTGCCAGTACTGTCAGCCTCTGCACCTTTTAA
AGCGGGCGGTCGCCACGGTACAACCAGCGATGCCGATCAATTTGTGCAAGTCGATAAAGGTCCACTGACCTTTAAAAACG
CCGCCGATCTGTACCTGTATCCCAATACTATGGTGGCCGTCAAAGCAACGGGTGCCGAATTGAAAGATTGGCTTGAGTGC
TCAGCCAACCAGTTCAATCAAATCGACCCAGCAAAAACCACCCCACAAGAGTTAATCAACTGGGATGGCCATCCAACCTA
TAATTTCGACGTACTCGATGGCTTAACTTACAAAATTGATGTCACTCAACCGAACAAGTTTGACCGCGATTGCGCCGTTG
TTAATGCCAACGCAAACCGTATCGTTGATTTAAGCTATAAAGACGAAAACGGCAAAGTCATCACAGGTGAAGAATTTGCC
GCGAAAGAGTTTATCGTCGCCTCAAATAACTATCGCGCCTTTGGTGGTAAGTTTGCCGGTACGGGTAGCGACCATGTCGT
GCTTGAACTGCCCGATACCAACCGTGAAGCATTAGCGGCTTATATCACAGCGCAATCTCAATACAACGAAACCACGGGTA
AGTACGAAGGTATGGTCAATCCAAGTGCCGATTACAACTGGGATTTCAAAACCATCACGACCAACGTCACATTAGACGTT
CGCTTCGAAACCCAAGACAGTGATAAAGCTGCTACCTTTATCGAAGCCAATAAACAACGTGAAATGAAGAAGATCAGCAA
GGATGAACTGGGTTTCGCCGTTTATAGCATCAACCTAACCAAATAA

Upstream 100 bases:

>100_bases
AAATTAACCTAACAGTTAAATGACGCCGAATTAACGGGATGATGGGCATGCTCTTGACGGAACATGCGTTGGGGTCAAAT
AAATATAAATAGAGATAACT

Downstream 100 bases:

>100_bases
GCGTTCAATCCCGAAATATCACCTCAAAAGGCTCCTCGTGAGCCTTTTTTATTCCCTACCGATTAAATTAACGCTAATCT
GCGCGCTATAAACGGCGTGT

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MLNKKILAVMITAALGVSACNSDDDKVVQADLRVLETTDLHTNIMDFNYYSGKDDPTIGLARTASLIHAARNEAKNSVLV
DNGDLLQGSPMGDYMAKVGLQMGDVHPAYKAMNLLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWK
EVKKGEHLFTPYIIKQKIIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIVTTAKKYVPEMKAKGADIIIAI
PHSGIGSTENPGDPMAENATYALTYVDGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLNGVPAVMPGRWGDNLGVVDFK
LERRDDKWHVLTAKTQARPIYDGANKAPLVEADAAIHDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSD
AQIANVKAKLPAALKGLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAELKDWLEC
SANQFNQIDPAKTTPQELINWDGHPTYNFDVLDGLTYKIDVTQPNKFDRDCAVVNANANRIVDLSYKDENGKVITGEEFA
AKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAQSQYNETTGKYEGMVNPSADYNWDFKTITTNVTLDV
RFETQDSDKAATFIEANKQREMKKISKDELGFAVYSINLTK

Sequences:

>Translated_681_residues
MLNKKILAVMITAALGVSACNSDDDKVVQADLRVLETTDLHTNIMDFNYYSGKDDPTIGLARTASLIHAARNEAKNSVLV
DNGDLLQGSPMGDYMAKVGLQMGDVHPAYKAMNLLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWK
EVKKGEHLFTPYIIKQKIIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIVTTAKKYVPEMKAKGADIIIAI
PHSGIGSTENPGDPMAENATYALTYVDGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLNGVPAVMPGRWGDNLGVVDFK
LERRDDKWHVLTAKTQARPIYDGANKAPLVEADAAIHDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSD
AQIANVKAKLPAALKGLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAELKDWLEC
SANQFNQIDPAKTTPQELINWDGHPTYNFDVLDGLTYKIDVTQPNKFDRDCAVVNANANRIVDLSYKDENGKVITGEEFA
AKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAQSQYNETTGKYEGMVNPSADYNWDFKTITTNVTLDV
RFETQDSDKAATFIEANKQREMKKISKDELGFAVYSINLTK
>Mature_681_residues
MLNKKILAVMITAALGVSACNSDDDKVVQADLRVLETTDLHTNIMDFNYYSGKDDPTIGLARTASLIHAARNEAKNSVLV
DNGDLLQGSPMGDYMAKVGLQMGDVHPAYKAMNLLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWK
EVKKGEHLFTPYIIKQKIIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIVTTAKKYVPEMKAKGADIIIAI
PHSGIGSTENPGDPMAENATYALTYVDGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLNGVPAVMPGRWGDNLGVVDFK
LERRDDKWHVLTAKTQARPIYDGANKAPLVEADAAIHDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSD
AQIANVKAKLPAALKGLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAELKDWLEC
SANQFNQIDPAKTTPQELINWDGHPTYNFDVLDGLTYKIDVTQPNKFDRDCAVVNANANRIVDLSYKDENGKVITGEEFA
AKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAQSQYNETTGKYEGMVNPSADYNWDFKTITTNVTLDV
RFETQDSDKAATFIEANKQREMKKISKDELGFAVYSINLTK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=678, Percent_Identity=52.9498525073746, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=502, Percent_Identity=21.7131474103586, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 74346; Mature: 74346

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKKILAVMITAALGVSACNSDDDKVVQADLRVLETTDLHTNIMDFNYYSGKDDPTIGL
CCCHHHHHHHHHHHHCHHCCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCCCCCEEH
ARTASLIHAARNEAKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDVHPAYKAMNLLDYEVG
HHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHEEECC
NIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWKEVKKGEHLFTPYIIKQKIII
CCCCCCCCCCHHHHHCCCCCCCCCEECCEEEEECCCCHHHHHHCCCEEECCEEEEEEEEE
DSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIVTTAKKYVPEMKAKGADIIIAI
ECCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEC
PHSGIGSTENPGDPMAENATYALTYVDGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLN
CCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHC
GVPAVMPGRWGDNLGVVDFKLERRDDKWHVLTAKTQARPIYDGANKAPLVEADAAIHDAV
CCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHH
AAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPAALKGLPVL
HHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCCCHHHCCCEEE
SASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAELKDWLEC
ECCCCCCCCCCCCCCCCCHHEEEECCCCEEECCCCEEEEECCEEEEEEECCHHHHHHHHH
SANQFNQIDPAKTTPQELINWDGHPTYNFDVLDGLTYKIDVTQPNKFDRDCAVVNANANR
CCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCEEEEEEECCCCCCCCCEEEEECCCCE
IVDLSYKDENGKVITGEEFAAKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAA
EEEEEEECCCCCEEECCCCCCCEEEEECCCEEECCCEECCCCCCEEEEECCCCCHHHEEE
YITAQSQYNETTGKYEGMVNPSADYNWDFKTITTNVTLDVRFETQDSDKAATFIEANKQR
EEEECCCCCCCCCCCCCEECCCCCCCCEEEEEEEEEEEEEEEECCCCCCCEEEEECHHHH
EMKKISKDELGFAVYSINLTK
HHHHCCHHHCCEEEEEEEECC
>Mature Secondary Structure
MLNKKILAVMITAALGVSACNSDDDKVVQADLRVLETTDLHTNIMDFNYYSGKDDPTIGL
CCCHHHHHHHHHHHHCHHCCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCCCCCEEH
ARTASLIHAARNEAKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDVHPAYKAMNLLDYEVG
HHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHEEECC
NIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWKEVKKGEHLFTPYIIKQKIII
CCCCCCCCCCHHHHHCCCCCCCCCEECCEEEEECCCCHHHHHHCCCEEECCEEEEEEEEE
DSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIVTTAKKYVPEMKAKGADIIIAI
ECCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEC
PHSGIGSTENPGDPMAENATYALTYVDGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLN
CCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHC
GVPAVMPGRWGDNLGVVDFKLERRDDKWHVLTAKTQARPIYDGANKAPLVEADAAIHDAV
CCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHH
AAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPAALKGLPVL
HHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCCCHHHCCCEEE
SASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAELKDWLEC
ECCCCCCCCCCCCCCCCCHHEEEECCCCEEECCCCEEEEECCEEEEEEECCHHHHHHHHH
SANQFNQIDPAKTTPQELINWDGHPTYNFDVLDGLTYKIDVTQPNKFDRDCAVVNANANR
CCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCEEEEEEECCCCCCCCCEEEEECCCCE
IVDLSYKDENGKVITGEEFAAKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAA
EEEEEEECCCCCEEECCCCCCCEEEEECCCEEECCCEECCCCCCEEEEECCCCCHHHEEE
YITAQSQYNETTGKYEGMVNPSADYNWDFKTITTNVTLDVRFETQDSDKAATFIEANKQR
EEEECCCCCCCCCCCCCEECCCCCCCCEEEEEEEEEEEEEEEECCCCCCCEEEEECHHHH
EMKKISKDELGFAVYSINLTK
HHHHCCHHHCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA