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The map label for this gene is estA [H]

Identifier: 160875813

GI number: 160875813

Start: 3209011

End: 3209682

Strand: Reverse

Name: estA [H]

Synonym: Sbal195_2701

Alternate gene names: 160875813

Gene position: 3209682-3209011 (Counterclockwise)

Preceding gene: 160875814

Following gene: 160875812

Centisome position: 60.02

GC content: 48.07

Gene sequence:

>672_bases
ATGTCAGTTTCTCTCGAGCGTATTGTCGTTGAACCTAAGACACCAGCTACAGCCGTGGTCATTTGGTTACATGGATTAGG
GGATTCGGGTGCCGGATTTGCCCCCGTAGTGCCTGCATTAGGTTTGCCTGCCGATCATGCTATCCGGTTTATTTTCCCCC
ATGCACCGGAGCAAGCCGTGACCATCAATGGCGGCTACGTCATGCGCGCTTGGTACGATATTAAAAGCATGGATTTGCAC
GACAGAGCTGATATGCAAGGCGTGCTTGAGTCGGAGAAAAGGGTTGCTGCATTAATTAATGAACAAATCGCGGCGGGTAT
TGCGAGCGAGCGTATCGTATTAGCAGGATTCAGTCAGGGCGGAGTTATGAGTTTATTCTCTGGACTGCGTTTCCCCGAGC
AACTTGCAGGGATTATGGCACTGTCTTGCTATTTACCGACGGGCGATGTATTACCCGCTGAATTAAGTGCAGCGAATCGT
AATACACCCATATTACAGCAACACGGTCTACAGGATGATGTAGTGCCATTATCCGCTGGAACATTAGCCAAAGAAGCCTT
AATTACGGGCGGTTATAATGTTGTGTGGCAGACTTATCCTATGCCGCATTCAGTGATCCCTGCGCAGTTAAAAGAGATTT
CTAAGTGGTTATTACAGCGATTTGAAATGTAG

Upstream 100 bases:

>100_bases
AGTACGCCTGCATAGTGCATAGTGCATAGTGGTTAACTTATAGATTTATCATCAGTTTTTAGTTGATGAGTGTTTAATAT
CGAATTGGGGATTTTTTAGT

Downstream 100 bases:

>100_bases
AGAAGTCTAATTGCTGATAAAAACAGCATGCGGTAAATTGAAAATTGCCCATGCTTGTTTTTTTATGGGAACAATCACTG
TTTTCTGTAATGTTTTCTAA

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSVSLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPADHAIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLH
DRADMQGVLESEKRVAALINEQIAAGIASERIVLAGFSQGGVMSLFSGLRFPEQLAGIMALSCYLPTGDVLPAELSAANR
NTPILQQHGLQDDVVPLSAGTLAKEALITGGYNVVWQTYPMPHSVIPAQLKEISKWLLQRFEM

Sequences:

>Translated_223_residues
MSVSLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPADHAIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLH
DRADMQGVLESEKRVAALINEQIAAGIASERIVLAGFSQGGVMSLFSGLRFPEQLAGIMALSCYLPTGDVLPAELSAANR
NTPILQQHGLQDDVVPLSAGTLAKEALITGGYNVVWQTYPMPHSVIPAQLKEISKWLLQRFEM
>Mature_222_residues
SVSLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPADHAIRFIFPHAPEQAVTINGGYVMRAWYDIKSMDLHD
RADMQGVLESEKRVAALINEQIAAGIASERIVLAGFSQGGVMSLFSGLRFPEQLAGIMALSCYLPTGDVLPAELSAANRN
TPILQQHGLQDDVVPLSAGTLAKEALITGGYNVVWQTYPMPHSVIPAQLKEISKWLLQRFEM

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=221, Percent_Identity=39.8190045248869, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI9966764, Length=204, Percent_Identity=38.2352941176471, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI20270341, Length=222, Percent_Identity=27.4774774774775, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508057, Length=211, Percent_Identity=35.5450236966825, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6323147, Length=210, Percent_Identity=33.8095238095238, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45553055, Length=215, Percent_Identity=37.2093023255814, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553057, Length=215, Percent_Identity=37.2093023255814, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21357257, Length=215, Percent_Identity=37.2093023255814, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI161078179, Length=214, Percent_Identity=30.8411214953271, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24038; Mature: 23907

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPADHAIRFIFPHAPEQAV
CCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHCCCCHHHEEEEEECCCCCCEE
TINGGYVMRAWYDIKSMDLHDRADMQGVLESEKRVAALINEQIAAGIASERIVLAGFSQG
EECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GVMSLFSGLRFPEQLAGIMALSCYLPTGDVLPAELSAANRNTPILQQHGLQDDVVPLSAG
CHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCHHHCCCCCCCCCHHHCCCCCCCEECCCC
TLAKEALITGGYNVVWQTYPMPHSVIPAQLKEISKWLLQRFEM
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSLERIVVEPKTPATAVVIWLHGLGDSGAGFAPVVPALGLPADHAIRFIFPHAPEQAV
CCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHCCCCHHHEEEEEECCCCCCEE
TINGGYVMRAWYDIKSMDLHDRADMQGVLESEKRVAALINEQIAAGIASERIVLAGFSQG
EECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GVMSLFSGLRFPEQLAGIMALSCYLPTGDVLPAELSAANRNTPILQQHGLQDDVVPLSAG
CHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCHHHCCCCCCCCCHHHCCCCCCCEECCCC
TLAKEALITGGYNVVWQTYPMPHSVIPAQLKEISKWLLQRFEM
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA