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The map label for this gene is rlmL [H]

Identifier: 160875797

GI number: 160875797

Start: 3188540

End: 3190669

Strand: Reverse

Name: rlmL [H]

Synonym: Sbal195_2685

Alternate gene names: 160875797

Gene position: 3190669-3188540 (Counterclockwise)

Preceding gene: 160875798

Following gene: 160875796

Centisome position: 59.67

GC content: 45.59

Gene sequence:

>2130_bases
ATGCTTAATTTTTTTGCCGCCGCCCCTAAGGGCTTTGAATATAGCTTAGCCCAAGAGCTGACAGAATTTGGTGCGACTGA
AGTAAAAGAGAGTGTTGCTGGTGTGTATTTCACGGCCTCCTTAGCTTTAGCGTATCGCATTACTTTATGGACACGCTTAG
CCAGCCGCATTGTGCTGGTTATCTATAAAGGAAGCTGCGAGTCGGCAGAACAACTCTATAATGCAGCTTATTGTGTTGAT
TGGCCGGCACACTTTTCCAATAAAAGCACTTTTAGTATCGATTTCCATGGTACGGGTGGTTTTCTTAATAATACCCAGTT
TGGCGCCTTGAAGATTAAGGATGCGATTGTCGATCGTTTCCGTGACGATGATATTGAGCGTCCGAATGTGTCTCGCGTTG
ATGCTGAATTTAAAGTCGATGCGCATTTCCGTAATGGCGTGATCACGATAGCGATGAACTTCTCGGGTCCATCGCTTCAT
CAACGTGGCTATCGTTCGACCACAGGTGAAGCGCCATTGAAGGAAAACTTAGCCGCCAACATGCTCGTACGCAGTGGCTG
GCAAGCGTCGCCTTCAACCTTGCTCGATCCTTTCTGTGGTAGCGGTACAGTGCTGATTGAAGCGGCGTTAATGGCGGCTG
ATATTGCCCCTGGTCTGCAACGTAGTCGTTTTGGTTTTGAACATTGGCGTCGCCATGATAAAGCCGTGTGGCAAGAAATC
GTTGAAGAAGCGAAAGCGCGTGCTTCATTAGGCGTGAAACGTTGCGAGATTAAATTCTACGGTTCTGATATCGATTCGCG
CTTAGTCGCGTTAGCGAAACGTAACGCCGAAAATGCCGGTGTACTGGAGTTAATCGAGTTTAAAGTTGCGGATGCTTTAA
CGATAGCGCCGCCTGCGGAAAGCGGCTATTTGATCACCAACCCACCTTATGGTGAGCGTTTAGGTAATGTGTCTGAGTTA
TTGCAGCTTTACTATCAGCTGGGCGACAAGTTTAAGAAAGAGTTTGGCGGCTGGAAAGTGGCCATGCTGTGTAGTGATAT
CGAACTGGTGTCATCGCTAAAGCTTAAAGCCGATAAACAAATGAAGATGTTTAACGGCGCACTTGAATGTGCTTTTAACA
TCTACACCTTACATGCCAACAGTACTCGCCGTGATACGCCAGTGCTGCCAGATGGCGTCGACATTGCTGATATTGCCCCA
GCTTTTGCTAACCGCATTAAGAAGAATGCCAAGTTATTAGAGAAATGGGCGAAGAAAGAAGGCATCGACAGCTATCGTAT
TTACGATGCCGATATTCCTGAATATAACGTCGCAGTAGATAAGTATTTAGATTACGTGATTATTCAAGAGTATATGGCGC
CAGCGACCATACCTGAAGCCGTGACTAAACGTCGTTTAAGTGATGTGTTATTAGCACTGCCATCGGCCATTGGCATCAAC
CCTAACAAGATGATCATGAAGACGCGTGAGCGTCAAAAGGGCACGAGTCAATATCAAAAGCTGGATGAGCGTAAGTTAGA
GCTTATCACTACCGAATATGGCGCTAAGTTCAAGTTGAACTTAACCGGTTATTTGGATACAGGTTTGTTCCTCGATCACA
GATTGACACGTCGTTTAGTCGGTCAAAAATCTAAAGGTCGCCGCGTACTGAACTTGTTCTCTTATACAGGTTCTGCTTCT
GTGCATGCAGCGCTTGGCGGTGCTAAGTCGGTAACGACAGTTGATATGTCTAATACCTATATCGCTTGGGCGAAGGACAA
CTTTGCGCTCAATGGCCTGCAAGGTAAGCAGTATGAGTTTGTTCAATCTGACTGCATGCAGTGGATTAGGGATTGTAATG
AACAGTACGATCTGATTTTTATCGATCCGCCAACCTTCTCAAATTCTAAGCGCATGGAAGATTCGTTTGATGTGCAACGG
GATCATGTGAACTTACTGGCATCCTTAGTAAAACTGCTCAGCCCAACGGGTGAGCTAGTGTTCTCTAACAATAAACGTAA
GTTTAAGATGGACATAGAGACGCTGACAAAGATGAACATTAATGTGACGAACATTGATGATGTGACTTTGCCTATGGACT
ACAAACGTAATCCACATATCCATAATACCTGGCTTATCACCCATGCCTGA

Upstream 100 bases:

>100_bases
AGACAGTGACTAAATGGGGCTTTGGCTAATAACTGTTTATCTTTTATGTATCCGAGCAGGTATAATGTCGCTTTGATTTT
GACTGCGTACTGATTATCCC

Downstream 100 bases:

>100_bases
GTTGACTCAAGCAGAGCGGGATAACAGGATTTATCTGCTATATCACACCGATGGCTGCCATCTGTGTGAACTGGCCGCGG
CGTTACTGGACGCGGCCGAC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLALAYRITLWTRLASRIVLVIYKGSCESAEQLYNAAYCVD
WPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRFRDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFKVADALTIAPPAESGYLITNPPYGERLGNVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQMKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAP
AFANRIKKNAKLLEKWAKKEGIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEFVQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQR
DHVNLLASLVKLLSPTGELVFSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA

Sequences:

>Translated_709_residues
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLALAYRITLWTRLASRIVLVIYKGSCESAEQLYNAAYCVD
WPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRFRDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFKVADALTIAPPAESGYLITNPPYGERLGNVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQMKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAP
AFANRIKKNAKLLEKWAKKEGIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEFVQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQR
DHVNLLASLVKLLSPTGELVFSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA
>Mature_709_residues
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLALAYRITLWTRLASRIVLVIYKGSCESAEQLYNAAYCVD
WPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRFRDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFKVADALTIAPPAESGYLITNPPYGERLGNVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQMKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAP
AFANRIKKNAKLLEKWAKKEGIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEFVQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQR
DHVNLLASLVKLLSPTGELVFSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=713, Percent_Identity=49.0883590462833, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=276, Percent_Identity=27.536231884058, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 79681; Mature: 79681

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLALAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IYKGSCESAEQLYNAAYCVDWPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRF
EECCCCHHHHHHHCCEEEEECCHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
HHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHH
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFKVADALTIAPPAE
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEECCCCC
SGYLITNPPYGERLGNVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQ
CCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAPAFANRIKKNAKLLEKWAKKE
HHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCEEEECCCCCHHHH
VQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLASLVKLLSPTGELV
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
FSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA
ECCCCEEEEEEHHHHHEECCEEECCCCEEECCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLALAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IYKGSCESAEQLYNAAYCVDWPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRF
EECCCCHHHHHHHCCEEEEECCHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
HHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHH
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFKVADALTIAPPAE
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEECCCCC
SGYLITNPPYGERLGNVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQ
CCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAPAFANRIKKNAKLLEKWAKKE
HHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCEEEECCCCCHHHH
VQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLASLVKLLSPTGELV
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
FSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA
ECCCCEEEEEEHHHHHEECCEEECCCCEEECCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA