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The map label for this gene is sucB [H]

Identifier: 160875739

GI number: 160875739

Start: 3111109

End: 3112299

Strand: Reverse

Name: sucB [H]

Synonym: Sbal195_2627

Alternate gene names: 160875739

Gene position: 3112299-3111109 (Counterclockwise)

Preceding gene: 160875740

Following gene: 160875738

Centisome position: 58.2

GC content: 47.86

Gene sequence:

>1191_bases
ATGAGTATCGAAATCAAGGTACCCGTACTACCAGAATCTGTTGCCGATGCCACGATTGCCACTTGGCATGTGAAAGTGGG
CGAGCAAGTTTCTCGTGATCAAAACCTGGTTGATATTGAAACCGATAAAGTGGTTCTTGAAGTGGTTGCACCAGAAGATG
GCCACATTGGCGAGTTTTTGTTTGAAGAAGGCGCCACTGTTCTGGGTGAACAAGTGATCGCTAAATTCATCGCCGGTGCT
GTATCTGGCAAAGAAGTCACTAAAGCGCAAGCTGAAGCGGCTACGCCTGCAGCTGAAACCAGCGATGAAAGCAGTGATGC
GTTAAGCCCATCTGTACGCCGTTTACTGGCTGAGCACAATGTTGATGCCAGCAAAGTAAAAGGCACAGGTGTGGGTGGTC
GTATTACTAAAGAAGACGTTGAAGCTTTTGTTAAGTCTGCGCCTAAAGCAGCGGCGCCAGCAGCACCTGCTGTGGCTCCA
TTAGCGGCTGGCCGTAGCGAGAAGCGCGTGCCAATGACACGTCTGCGTAAGACAATCGCTAACCGCTTGTTAGAAGCGAA
AAACTCTACCGCTATGCTCACCACGTTTAACGAAGTGAACATGAAGCCAATCATGGATATCCGTAAGCAATACCAAGATA
TCTTTGAAAAGCGTCACGGTATCCGTTTAGGCTTTATGTCTTTCTACATTAAAGCCGTGACTGAAGCGCTGAAACGTTTC
CCTGAAGTGAACGCGTCTATTGATGGCGATGATATCGTTTATCACAACTACTTCGACATCAGCATTGCTGTATCAACGCC
ACGTGGTCTGGTAACACCAGTATTACGTGACACTGATACCATGAACTTAGCTGAAATCGAAAAAGCTGTCCGTGACTTAG
CCCTTAAAGGCCGTGACGGTAAGCTGACTGTGGCTGACATGACTGGCGGTAACTTCACTGTGACTAACGGTGGTGTTTTC
GGTTCACTGATGTCTACACCGATTCTTAACCTGCCACAAAGCGCGATTTTAGGCATGCATGCCATTAAAGATCGCCCAAT
GGCAGTCAATGGTCAGGTTGAAATCCTGCCCATGATGTACCTGGCACTCTCATATGACCATCGTATTGTTGATGGCCGTG
AGTCTGTTGGTTTCTTGGTGGCGATTAAAGACTTCCTAGAAGACCCAACTCGTCTGCTGCTTGATCTGTAA

Upstream 100 bases:

>100_bases
CCTGAGTTGCACGCACATCAGCAAGAATCTTTAGTGAACAGTGCCCTGAAACTTACAATCACACTGTAGTAATAGACATT
TTATAAAAGGATAGTTTTTC

Downstream 100 bases:

>100_bases
GACAACTCGATAAGAGTTTGCGCTTAAAGAAATAGCCAGACATACCCTCGTGACTGGCCCAAACGGGCCAGTTGGATTTG
ATTAGAAGTATACGGATAGA

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFEEGATVLGEQVIAKFIAGA
VSGKEVTKAQAEAATPAAETSDESSDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFVKSAPKAAAPAAPAVAP
LAAGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYIKAVTEALKRF
PEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMNLAEIEKAVRDLALKGRDGKLTVADMTGGNFTVTNGGVF
GSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL

Sequences:

>Translated_396_residues
MSIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFEEGATVLGEQVIAKFIAGA
VSGKEVTKAQAEAATPAAETSDESSDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFVKSAPKAAAPAAPAVAP
LAAGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYIKAVTEALKRF
PEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMNLAEIEKAVRDLALKGRDGKLTVADMTGGNFTVTNGGVF
GSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
>Mature_395_residues
SIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFLFEEGATVLGEQVIAKFIAGAV
SGKEVTKAQAEAATPAAETSDESSDALSPSVRRLLAEHNVDASKVKGTGVGGRITKEDVEAFVKSAPKAAAPAAPAVAPL
AAGRSEKRVPMTRLRKTIANRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYIKAVTEALKRFP
EVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMNLAEIEKAVRDLALKGRDGKLTVADMTGGNFTVTNGGVFG
SLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=55.0607287449393, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.5747126436782, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=26.6666666666667, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=28.0973451327434, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=28.476821192053, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=71.712158808933, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=33.6609336609337, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=42.2222222222222, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=31.4942528735632, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=25.7683215130024, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=29.8412698412698, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=406, Percent_Identity=41.871921182266, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=24.9443207126949, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=59.0717299578059, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=416, Percent_Identity=29.3269230769231, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24582497, Length=290, Percent_Identity=33.7931034482759, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=33.7931034482759, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42881; Mature: 42750

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFL
CEEEEEECCCCCHHCCCEEEEEEEECCHHHHCCCCEEEEECCEEEEEEECCCCCCHHHHH
FEEGATVLGEQVIAKFIAGAVSGKEVTKAQAEAATPAAETSDESSDALSPSVRRLLAEHN
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
VDASKVKGTGVGGRITKEDVEAFVKSAPKAAAPAAPAVAPLAAGRSEKRVPMTRLRKTIA
CCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
NRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYIKAVTEALKRF
HHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHC
PEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMNLAEIEKAVRDLALKGRDG
CCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
KLTVADMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMY
EEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHHHHHH
LALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
HHHCCCCEEECCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SIEIKVPVLPESVADATIATWHVKVGEQVSRDQNLVDIETDKVVLEVVAPEDGHIGEFL
EEEEEECCCCCHHCCCEEEEEEEECCHHHHCCCCEEEEECCEEEEEEECCCCCCHHHHH
FEEGATVLGEQVIAKFIAGAVSGKEVTKAQAEAATPAAETSDESSDALSPSVRRLLAEHN
HHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
VDASKVKGTGVGGRITKEDVEAFVKSAPKAAAPAAPAVAPLAAGRSEKRVPMTRLRKTIA
CCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
NRLLEAKNSTAMLTTFNEVNMKPIMDIRKQYQDIFEKRHGIRLGFMSFYIKAVTEALKRF
HHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHC
PEVNASIDGDDIVYHNYFDISIAVSTPRGLVTPVLRDTDTMNLAEIEKAVRDLALKGRDG
CCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
KLTVADMTGGNFTVTNGGVFGSLMSTPILNLPQSAILGMHAIKDRPMAVNGQVEILPMMY
EEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEHHHHHH
LALSYDHRIVDGRESVGFLVAIKDFLEDPTRLLLDL
HHHCCCCEEECCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]