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The map label for this gene is cysB [H]

Identifier: 160875719

GI number: 160875719

Start: 3086847

End: 3087830

Strand: Reverse

Name: cysB [H]

Synonym: Sbal195_2607

Alternate gene names: 160875719

Gene position: 3087830-3086847 (Counterclockwise)

Preceding gene: 160875721

Following gene: 160875716

Centisome position: 57.75

GC content: 45.22

Gene sequence:

>984_bases
ATGAAACTGCAACAACTCAGGTATATTGCTGAGGTCGTAAAACATAACCTCAATGTATCGGCGACAGCTGAAAACTTGTA
TACCTCTCAACCTGGGATCAGTAAACAAGTTCGCATGCTTGAAGATGAGTTGGGGATCCAAATATTTGGTCGTAGCGGTA
AGCACTTAACCCATGTCACACCTGCGGGTTTGCAAGTGATCAGCATTGCCAATGATATCTTGGGTAAAGTCGAGAGCATT
AAAAAAGTTTCTGAGGAATATACTAAGCCCGATCAAGGCGAGTTAAATATTTCCACGACAGACACTCAGGCGCGCTATGC
ATTGCCGACGATCATTCGGCAGTTTATTGATCGCTACCCTAAAGTGAACTTGCATATGCATCAGGGGACGCCGTCGCAGA
TCAGCGAACAGGCCGCTCGCGGTGATGCGGATTTCGCCATCGCGACTGAAGCAATGCATTTGTATTCCGACTTGATTATG
TTGCCTTGTTATCATTGGAATCGCTCCATCGTCGTTCCTAAAGATCATCCTCTTGCGACCCGTAGCCCAAATAATTTGAT
TAGCATCGAAGATTTAGCGCGCTTCCCCTTAGTGACTTATGTCTTTGGTTTTGACCGTGCTTCTGAAATTGAGAAAGCGT
TCAATCGCGCCAATTTAGAGCCGCGTGTTGTTTTTAGTGCGACCAGCGCCGATGTGTTGAAAACCTATGTCCGTTTAGGA
TTAGGTGTGGGTGTTATTGCCTCTATGGCGATTGACCCTAATGTCGATAAAGATTTAGTTGCGATTGATGCTGGCCATTT
GTTTGCCCACAGTACGACTAAAATTGGCTTCAGGAAGGGTAGCTTCTTACGCAGTTATATGTATGATTTTATTCAGCACT
TTGCGCCGCATTTAACCCGTGACGTGGTGGAGAAAGCTGTCGCCTTACGGGATCAACAGCTTATTGATGAAATGTTTGCC
GATAAAAATCTGCCAATGCGTTAA

Upstream 100 bases:

>100_bases
TTTAAGTTCTACTATATACTATAACGATTGATTATAGCTGAGAGTGCTTTGAGGGCTCAGTTGTTAATGGCATTTTGCGT
GTTTGAAACAAGGAAATAAG

Downstream 100 bases:

>100_bases
TTGGCCATAATTTACTGACATAAAAATGCCGCTAATATAAGCAGCATTTTTATGGAATAATGTTTAGCTGAAACTTCATT
TATAAATCAAGGTATTAAAC

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGLQVISIANDILGKVESI
KKVSEEYTKPDQGELNISTTDTQARYALPTIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSIVVPKDHPLATRSPNNLISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLG
LGVGVIASMAIDPNVDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFA
DKNLPMR

Sequences:

>Translated_327_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGLQVISIANDILGKVESI
KKVSEEYTKPDQGELNISTTDTQARYALPTIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSIVVPKDHPLATRSPNNLISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLG
LGVGVIASMAIDPNVDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFA
DKNLPMR
>Mature_327_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGLQVISIANDILGKVESI
KKVSEEYTKPDQGELNISTTDTQARYALPTIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSIVVPKDHPLATRSPNNLISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLG
LGVGVIASMAIDPNVDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFA
DKNLPMR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=327, Percent_Identity=69.7247706422018, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1788296, Length=316, Percent_Identity=37.3417721518987, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=25.9515570934256, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI157672245, Length=251, Percent_Identity=27.8884462151394, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=26.1168384879725, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789204, Length=229, Percent_Identity=22.707423580786, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=26.8456375838926, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36707; Mature: 36707

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGLQVISIANDILGKVESIKKVSEEYTKPDQGELNISTTDTQARYALPTIIRQFIDRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
KVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIMLPCYHWNRSIVVPKDHPLAT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEECCCCCCCCC
RSPNNLISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLG
CCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
LGVGVIASMAIDPNVDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTR
CCHHHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DVVEKAVALRDQQLIDEMFADKNLPMR
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGLQVISIANDILGKVESIKKVSEEYTKPDQGELNISTTDTQARYALPTIIRQFIDRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
KVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIMLPCYHWNRSIVVPKDHPLAT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEECCCCCCCCC
RSPNNLISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLG
CCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
LGVGVIASMAIDPNVDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTR
CCHHHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DVVEKAVALRDQQLIDEMFADKNLPMR
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]