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The map label for this gene is ppsA [H]

Identifier: 160875713

GI number: 160875713

Start: 3076801

End: 3079170

Strand: Reverse

Name: ppsA [H]

Synonym: Sbal195_2601

Alternate gene names: 160875713

Gene position: 3079170-3076801 (Counterclockwise)

Preceding gene: 160875716

Following gene: 160875712

Centisome position: 57.58

GC content: 46.58

Gene sequence:

>2370_bases
GTGCAGCAATACGTACTCTGGTATCAAGAATTAGGCATGGGTGACGTCAACTTGGTTGGCGGTAAAAATGCTTCTCTAGG
CGAGATGATCAGCAATCTGGCCAATGCCGGTGTTCAAGTACCTGGCGGATTTGCGACAACTTCCCATGCGTTCAACGAGT
TCCTTGAACAAAGCGGAGTTAACCAGAAGATTTATGACATTCTAGCCACACTGGATGTAGATGATGTCAATGCGCTGGCT
AAAGTTGGTGCACAGATCAGGCAGTGGGTTATCGACACCCCATTTCAACCAGCATTAGAGCAAGCCATTCGTGAAGCTTA
CGATAAACTTGCCGCTGAAACTAGCGAAGCGTCATTCGCGGTACGTTCTTCTGCAACAGCTGAAGATATGCCAGATGCGT
CTTTTGCGGGTCAGCAAGAAACTTTCCTAAACGTTAAAGGTTTTGAATCTGTACTGACGGCTATCAAGCACGTTTACGCT
TCACTCTTTAACGACCGCGCGATTTCTTACCGTGTTCACCAAGGTTACGAACACCACGGTGTTGCCTTGTCTGCTGGCGT
GCAACGCATGGTTCGTTCAGACAAAGCGGCTTCAGGTGTGATGTTCACTATGGACACTGAATCTGGCAATAACGATGTCG
TGTTTATCACTTCATCTTTTGGTCTGGGTGAAATGGTTGTTCAAGGCGCGGTAAACCCTGATGAATTTTATGTTCATAAA
CCTATTTTAGCCTTAGGTCACAAGGCTGTTGTGCGCCGTAATATCGGTAGCAAGCTCATCCAAATGGTGTATTCAGATGA
TGCTGCTCACGGCAAACAAGTCAAAATTGAAGATGTTGCAAGCGAGCAACGCCGTCAATTCTCAATCAATGACGCTGAAG
TTGAAGAGTTGGCAAAACAAGCTGTGATTATCGAGCAACACTATGGTCGTCCAATGGACATCGAGTGGGCAAAAGACGGT
AACGACGGTAAATTGTATATCGTTCAAGCGCGTCCAGAAACGGTTCGTAGCCGTGAAGATGTGCAATTGATTGAGCGTTA
TCACTTAAAGAGCCGTGGCGCTGTGATCTGTGAAGGTCGCGCTATCGGTCATAAAGTGGGAACCGGCGTAGCCAAAGTAT
TAACCTCTATCGCTGATATGGATCAAATCCAACCAGGTGACGTGTTAGTGACTGACATGACAGATCCAGATTGGGAACCT
ATCATGAAACGCGCGAGCGCCATCGTCACTAACCGTGGCGGTCGTACTTGTCACGCGGCGATTATCGCCCGTGAATTAGG
TGTGCCAGCGGTGGTGGGTTGTGGTGACGTGACTGACCGTATCAAGAATGGTCAAATCGTGACTGTTTCTTGCGCTGAAG
GTGACACTGGCTTTATCTACGAAGGTAAACAAGAGTTTGAAGTGATTTCAAACCGTGTTGACTCTTTGCCAGAACTGCCA
ATGAAAATCATGATGAACGTGGGTAACCCTGATCGTGCATTTGATTTCGCCCGTTTACCAAACGAAGGTGTGGGTCTAGC
GCGTTTAGAATTCATCATCAACCGTATGATTGGTATTCATCCAAAGGCACTGCTCGAGTTCAATCAACAGGATGCAGCAC
TGCAAACTGAAATCAACGAGATGATTGCTGGTTATGAATCACCGGTTGAGTTCTACATTGCCCGTCTGGTTGAAGGTATT
GCGACTATCGGTTCTGCTTTCTATCCGAAGAAAGTGATCGTACGTATGTCAGACTTTAAGTCTAACGAATACGCAAACTT
GGTTGGCGGTGACAGATACGAACCAGAAGAAGAGAACCCAATGTTGGGCTTCCGCGGTGCAAGCCGTTATATCTCTGAAT
CGTTCCGTGACTGTTTTGCCTTAGAGTGTGAAGCGATCAAACGTGTTCGTAACGACATGGGTCTGAAAAACGTTGAAGTC
ATGATCCCGTTCGTACGTACAGTGAAAGAAGCTGAACAAGTGATTGGCCTGCTGAAAGAGCAAGGTTTAGAGCGCGGTAA
AGATGGTTTACGCGTCATCATGATGTGTGAGGTGCCTTCAAACGCTCTGTTAGCGGATCAATTCCTTGAGCATTTCGATG
GTTTCTCTATCGGCTCAAACGATTTAACTCAGCTGACACTCGGTCTTGACCGTGACTCAGGTATCATCAGCCACTTATTT
GATGAGCGTGATGAAGCCGTTAAAATGCTACTTTCTTTAGCCATTAAAGCGGCTAAAACTAAGGGCGCATACATAGGTAT
TTGTGGTCAAGGTCCTTCAGATCACGCCGATTTTGCGGCTTGGTTAGTTGAGCAGGGCATAGATACCGTATCGTTAAATC
CAGACACTGTGATTGATACTTGGTTGTATTTAGCCGAAGCTCACGGTTGA

Upstream 100 bases:

>100_bases
TACAAATAAAATTATAGATTTACGCGTGTTCGACAGGAGTGTAAAATGCCGCTGCCCTCGTGTGAAGGGGCCACTGAAAT
AAACTTGCGGAGATAGAACT

Downstream 100 bases:

>100_bases
TCTAGATTGTAATTTTATAAACTTGTTAAAAAAGCCGCTTTTTAGCGGCTTTTTTTGTTTATAATGGCGCCATAACGATA
ATTGACAGAAGCCATCTATG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSHAFNEFLEQSGVNQKIYDILATLDVDDVNALA
KVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSEASFAVRSSATAEDMPDASFAGQQETFLNVKGFESVLTAIKHVYA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILALGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVASEQRRQFSINDAEVEELAKQAVIIEQHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVICEGRAIGHKVGTGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQIVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPELP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQTEINEMIAGYESPVEFYIARLVEGI
ATIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEV
MIPFVRTVKEAEQVIGLLKEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERDEAVKMLLSLAIKAAKTKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Sequences:

>Translated_789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSHAFNEFLEQSGVNQKIYDILATLDVDDVNALA
KVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSEASFAVRSSATAEDMPDASFAGQQETFLNVKGFESVLTAIKHVYA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILALGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVASEQRRQFSINDAEVEELAKQAVIIEQHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVICEGRAIGHKVGTGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQIVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPELP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQTEINEMIAGYESPVEFYIARLVEGI
ATIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEV
MIPFVRTVKEAEQVIGLLKEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERDEAVKMLLSLAIKAAKTKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG
>Mature_789_residues
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSHAFNEFLEQSGVNQKIYDILATLDVDDVNALA
KVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSEASFAVRSSATAEDMPDASFAGQQETFLNVKGFESVLTAIKHVYA
SLFNDRAISYRVHQGYEHHGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PILALGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVASEQRRQFSINDAEVEELAKQAVIIEQHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVICEGRAIGHKVGTGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIKNGQIVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPELP
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQTEINEMIAGYESPVEFYIARLVEGI
ATIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEV
MIPFVRTVKEAEQVIGLLKEQGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERDEAVKMLLSLAIKAAKTKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=74.6803069053708, Blast_Score=1254, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=456, Percent_Identity=27.8508771929825, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI48994992, Length=195, Percent_Identity=32.8205128205128, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1789193, Length=403, Percent_Identity=25.0620347394541, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=329, Percent_Identity=26.4437689969605, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86739; Mature: 86739

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSHAFNEFLEQSGV
CCCCEEEHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NQKIYDILATLDVDDVNALAKVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSEASFA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHEE
VRSSATAEDMPDASFAGQQETFLNVKGFESVLTAIKHVYASLFNDRAISYRVHQGYEHHG
ECCCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PILALGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVASEQRRQFSINDAEVEELAKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHH
AVIIEQHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVICEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHCCCCCEEECCC
AIGHKVGTGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTDRIKNGQIVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPELP
HHHHHCCCCEEEECCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQTEINE
HHHHEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
MIAGYESPVEFYIARLVEGIATIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEVMIPFVRTVKEAEQVIGLLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
HHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERDEAVKMLLSLAIKAAKTKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDT
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC
>Mature Secondary Structure
MQQYVLWYQELGMGDVNLVGGKNASLGEMISNLANAGVQVPGGFATTSHAFNEFLEQSGV
CCCCEEEHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NQKIYDILATLDVDDVNALAKVGAQIRQWVIDTPFQPALEQAIREAYDKLAAETSEASFA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHEE
VRSSATAEDMPDASFAGQQETFLNVKGFESVLTAIKHVYASLFNDRAISYRVHQGYEHHG
ECCCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PILALGHKAVVRRNIGSKLIQMVYSDDAAHGKQVKIEDVASEQRRQFSINDAEVEELAKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHH
AVIIEQHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKSRGAVICEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHHCCCCCEEECCC
AIGHKVGTGVAKVLTSIADMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTDRIKNGQIVTVSCAEGDTGFIYEGKQEFEVISNRVDSLPELP
HHHHHCCCCEEEECCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
MKIMMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQTEINE
HHHHEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
MIAGYESPVEFYIARLVEGIATIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEVMIPFVRTVKEAEQVIGLLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
HHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERDEAVKMLLSLAIKAAKTKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTVIDT
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]