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The map label for this gene is trmJ [H]

Identifier: 160875617

GI number: 160875617

Start: 2955788

End: 2956516

Strand: Reverse

Name: trmJ [H]

Synonym: Sbal195_2505

Alternate gene names: 160875617

Gene position: 2956516-2955788 (Counterclockwise)

Preceding gene: 160875619

Following gene: 160875616

Centisome position: 55.29

GC content: 49.11

Gene sequence:

>729_bases
ATGCTAAGTAATATTCGAGTGGTTTTAGTGGGGACTTCGCACCCCGGCAATATTGGTTCCACTGCCAGAGCGATGAAGAC
TATGGGTCTGTCAAATCTGTACCTTGCAGAACCGCGTGTTGAACCCGATGGGCATTCCATTGCATTAGCCGCGGGTGCAT
CGGATATTCTGAAACATCTTGTGAAAGTGGATTCACTTGCTGAAGCGATTGCCGATTGTAGTCTAGTGATCGCAACCAGC
GCGCGTAGTCGTACCTTAGATTGGCCGATGTTAGAGCCAAGAGAAGCAGGGGTGAGATTAGCGACCGAGGCACTTAAAGG
CCCCGTTGCAATCGTGTTTGGCCGTGAGAACCACGGGTTGAGCAATGAAGAGTTGCAACAGTGCACTTATCATGTGGCGA
TTCCTGCAAATCCTGAATACAGCTCGCTCAATCTTGCGCAAGCAGTACAAATCATTTGTTATGAAACCCGTGTTGCCCAC
CTCGCACAAAAAGAAGTGGCCGAAGAAGAGCTGACGGAGTATCCCTTGGCGGCCGATCAGGAACGTTTCTTTGCACACCT
TGAGACGACACTGCTATCAACCGGCTTTATCATTAAGAATCATCCCGGACAAGTGATGATGAAACTGCGCCGCTTGTTTA
GCCGCGCACGGATTGAGAGTCAGGAGATGAATATCCTCAGGGGTATTTTGACCTCTATTGAGAAAGTGACGGGTTCTAAA
GACAAATAG

Upstream 100 bases:

>100_bases
ATTTGATAGTTATATCAAATACCGATTTTCATATAAGCATTCGGCAATGGCTGTGATAATATTCGTCCCCAACGTTTTAT
GAATTAAATTAAGTTGTTTC

Downstream 100 bases:

>100_bases
CCAAATCCTCATCATCAATAATTACCGTACAGAATGACCGTAAAGGGATAAACAATGGGCGTGATAGCTAGGCTAAAAGA
AGACATTGAATCTATATATC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSNLYLAEPRVEPDGHSIALAAGASDILKHLVKVDSLAEAIADCSLVIATS
ARSRTLDWPMLEPREAGVRLATEALKGPVAIVFGRENHGLSNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAQKEVAEEELTEYPLAADQERFFAHLETTLLSTGFIIKNHPGQVMMKLRRLFSRARIESQEMNILRGILTSIEKVTGSK
DK

Sequences:

>Translated_242_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSNLYLAEPRVEPDGHSIALAAGASDILKHLVKVDSLAEAIADCSLVIATS
ARSRTLDWPMLEPREAGVRLATEALKGPVAIVFGRENHGLSNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAQKEVAEEELTEYPLAADQERFFAHLETTLLSTGFIIKNHPGQVMMKLRRLFSRARIESQEMNILRGILTSIEKVTGSK
DK
>Mature_242_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSNLYLAEPRVEPDGHSIALAAGASDILKHLVKVDSLAEAIADCSLVIATS
ARSRTLDWPMLEPREAGVRLATEALKGPVAIVFGRENHGLSNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAQKEVAEEELTEYPLAADQERFFAHLETTLLSTGFIIKNHPGQVMMKLRRLFSRARIESQEMNILRGILTSIEKVTGSK
DK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=67.6470588235294, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1790865, Length=242, Percent_Identity=32.2314049586777, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26512; Mature: 26512

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSNLYLAEPRVEPDGHSIALAAGASDILKHL
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCHHHHHHHH
VKVDSLAEAIADCSLVIATSARSRTLDWPMLEPREAGVRLATEALKGPVAIVFGRENHGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
SNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAHLAQKEVAEEELTEYPLAADQ
CHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ERFFAHLETTLLSTGFIIKNHPGQVMMKLRRLFSRARIESQEMNILRGILTSIEKVTGSK
HHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
DK
CC
>Mature Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSNLYLAEPRVEPDGHSIALAAGASDILKHL
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCHHHHHHHH
VKVDSLAEAIADCSLVIATSARSRTLDWPMLEPREAGVRLATEALKGPVAIVFGRENHGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
SNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAHLAQKEVAEEELTEYPLAADQ
CHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ERFFAHLETTLLSTGFIIKNHPGQVMMKLRRLFSRARIESQEMNILRGILTSIEKVTGSK
HHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
DK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA