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The map label for this gene is thiH [H]

Identifier: 160875546

GI number: 160875546

Start: 2878836

End: 2879951

Strand: Reverse

Name: thiH [H]

Synonym: Sbal195_2434

Alternate gene names: 160875546

Gene position: 2879951-2878836 (Counterclockwise)

Preceding gene: 160875547

Following gene: 160875545

Centisome position: 53.86

GC content: 47.49

Gene sequence:

>1116_bases
ATGAGTTTTGTAGCGGAATTTGCCAACATCCCACGGGATAAACTGTTACTCGATTTGTATTCTTGCACTGCCCAAGATGT
TGAGCGGGCGCTGGTGAGTCCAGCAGGGGATTTACGTAGCTTATTGGCCTTGCTATCACCTGCGGCGGAACCTTATATCG
AAACCATGGCGCAGCACTCTGCGGCGCTAACGCGGCAGCGATTTGGCGCGAATCTGGGCATGTATTTACCGCTATACGTC
TCGAATCTGTGCGCTAATGAGTGTGATTACTGTGGCTTTAGCATGAGTAACAAACTCAAACGCAAGACCTTGAATGAGCA
GGAGTTGATGGCCGAAATGGCCATTATCAAAGATAGGGGCTTCGATTCTATTTTACTGGTGTCAGGTGAGCATGAAACTA
AGGTCGGCATCGATTATTTCAAGCAAATGTTGCCGCTGGTAAAGCAGCAATTTAGCCATTTGGCGATGGAGGTCCAGCCC
ATGAGTGAGGACCATTATTGCCAGTTAGTCGCGCTAGGTTTAGATGCTGTGATGATTTATCAGGAGACCTATCAGCCCGA
AACTTATGCTCGTCACCATTCTCGAGGGAAAAAAATGGATTTTGCCTATCGTTTAGCAACACCGGACAGAGTTGCGGCGG
CGGGCGTCGATAAAATTGGTCTTGGGGTATTACTCGGGCTGGATGATTGGCGTTTAGATGCGTTAATGATGGGATATCAT
CTCGACTATTTAGAGCGACGCTATTGGCGCACCCGCTTTAGTATTTCGCTACCAAGGTTACGACCTTGTACTGGCGGGAT
CACCCCAAAAGTCATGCTATCGGATCTAGGTTTAGTGCAAATGATTTGTGCATTTAGACTTTTTAATCAACAGCTTAACA
TCAGCATGTCGACAAGGGAAAGCCCTGAGCTGAGGGATAATCTTTTGCCACTTGGGATCACTCAAATCAGTGCGGGCAGT
TCGACACAACCGGGCGGCTATCAAGCGCCTGACAGTCAACTCGATCAATTTGAGATAAGCGATGATCGCAGTGTCGAGCA
AGTTATCGAACAGATGCAACGGCAGGGTTTTAATCCCGTATTTAAGGATTGGGAAGCTAATTGGATCACAGGATAA

Upstream 100 bases:

>100_bases
AAGAGGCGGTGCAATGCGGTAGACGGGCCTTTGAAGCTGGGCTTGGCCGAGTACAAACGGGCGCGGTACATACCAGTCCT
CTGACGGGATTTTTAAACCA

Downstream 100 bases:

>100_bases
ATCTGGCCGTAAATAAATGCTGAGAATGCGGATAAAATCTGTACTTTTGCACGTTGCTGGTCAATAATTAACCCATTAAT
CACAGCGTGCAGGAATACCG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSFVAEFANIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHSAALTRQRFGANLGMYLPLYV
SNLCANECDYCGFSMSNKLKRKTLNEQELMAEMAIIKDRGFDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQP
MSEDHYCQLVALGLDAVMIYQETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLNISMSTRESPELRDNLLPLGITQISAGS
STQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPVFKDWEANWITG

Sequences:

>Translated_371_residues
MSFVAEFANIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHSAALTRQRFGANLGMYLPLYV
SNLCANECDYCGFSMSNKLKRKTLNEQELMAEMAIIKDRGFDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQP
MSEDHYCQLVALGLDAVMIYQETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLNISMSTRESPELRDNLLPLGITQISAGS
STQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPVFKDWEANWITG
>Mature_370_residues
SFVAEFANIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHSAALTRQRFGANLGMYLPLYVS
NLCANECDYCGFSMSNKLKRKTLNEQELMAEMAIIKDRGFDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQPM
SEDHYCQLVALGLDAVMIYQETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYHL
DYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLNISMSTRESPELRDNLLPLGITQISAGSS
TQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPVFKDWEANWITG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=52.7322404371585, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41950; Mature: 41819

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVAEFANIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHS
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
AALTRQRFGANLGMYLPLYVSNLCANECDYCGFSMSNKLKRKTLNEQELMAEMAIIKDRG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
FDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQPMSEDHYCQLVALGLDAVMIY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHEEE
QETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
HHHCCCHHHHHHHCCCCCCCEEEEECCCHHHHHCCCCHHCCEEEECCCCHHHHHHHHHHH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLNISMSTRE
HHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCEEECCCC
SPELRDNLLPLGITQISAGSSTQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPV
CCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCHH
FKDWEANWITG
HHCCCCCCCCC
>Mature Secondary Structure 
SFVAEFANIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHS
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
AALTRQRFGANLGMYLPLYVSNLCANECDYCGFSMSNKLKRKTLNEQELMAEMAIIKDRG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
FDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQPMSEDHYCQLVALGLDAVMIY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHEEE
QETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
HHHCCCHHHHHHHCCCCCCCEEEEECCCHHHHHCCCCHHCCEEEECCCCHHHHHHHHHHH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLNISMSTRE
HHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCEEECCCC
SPELRDNLLPLGITQISAGSSTQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPV
CCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCHH
FKDWEANWITG
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]