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The map label for this gene is btuB [H]

Identifier: 160875530

GI number: 160875530

Start: 2855821

End: 2858382

Strand: Reverse

Name: btuB [H]

Synonym: Sbal195_2418

Alternate gene names: 160875530

Gene position: 2858382-2855821 (Counterclockwise)

Preceding gene: 160875531

Following gene: 160875528

Centisome position: 53.45

GC content: 42.78

Gene sequence:

>2562_bases
ATGATCAAAAGAACCAAAGTAGCGTCGGCTATTAACTTAGCTATCGTTGCATCTATCACTTCTGGTGCATTTGTAACGTC
AAGCGCATTTGCAGCGGAAGAAAAAGCCAAAGTTGAACGTATTGAAGTCACGGGTTCACGCATTCAGCGCCAAGATATGG
AAACAGCTTCACCTGTGACCGTTATTGATGCTGCTGCGATTAAAGCGGAAGGTTTTACTTCTGTTGACCAAATGCTACAA
GTACAAACATCTATGGCGGGTGCAGCCGTGGGTTCAAGCACTAACAATGGTGCTGATGGCGTCGCGCAAGTTGATTTACG
TGGTATGGGTTCACAGCGCACGCTTGTATTACTGAATGGCCGTCGTATGGTGAACTCAGGTTCTGGTGCTGATAGTGCAG
TGGATCTGAACTCAATCCCTGTGGCTATGATCGCACGTGTTGAAATTCTGAAAGATGGCGCCTCAGCGGTTTATGGTTCA
GATGCGATTGCCGGTGTAGTGAACATTATCACTAAGAAAGACTTTGAAGGTTTCCAGCTCGATTTTAACGGCAGTGGCAC
TGACAAAGGTGACGGTGAGAGTGGTGATGTAAGTGCATTATATGGTTTCAACACAGAGGGTGGTAACTACACCTTTGGTG
CTGCTTATGCTGACCGTCGTGGTGTTATCCAAGGTGATCGAGATTGGTATGACCCACTCGATAATACGAGTTCAATTATT
CCAACTGGTGCATTAAACCAGAAGGTTATTGATGCAAATGGTAATTGGGTTAACAGAGCCACAGGTTATGATTATTCGAA
TGACGTTTACTTCCAAACGCCAAGCGAACGCCGTAGCTTATTTGCAAATATGACACAAGAGTTAGGTAATGATGTGGTAC
TAACCGCGGATGCTATCTATACCAATCGCCGTTCAAACCAACAGCTTGCGGCACAGCCTGCGCAAATCAAATTAAATGTT
TGTGGCGACCCAAGAGTGACAGATCCAACTGCTGGTTGCCTTAATCTTGACCAAAGCATGGTTGATGCAGGGATTAAAGC
TGACGCTCGCGGACAAGTGCAATATAACAGACGTATGAATGATGTTGGACCTCGTGTTTACAGCCAAGATACTGATACTT
GGCGTTTATCTGGTGGATTAGCGGGTTCTTTGGATGTTCATACTGGTATGAACTGGAATGTGGACTACACATTTGGTAAG
AACGAGGCTAAAACTGGTGTTGATAACTCAATTAACGCAACATTAATGGCTGCTTCTATTTATGCAGACCCATATGCGTG
GTTTGAAGGGTCTCCACTGACGAATGAAATGAAAGACGTTTCGTATGACGAGAAATCAACTGGTGGCAATGAACAACAAA
CGTTGTCTGCTGGCTTAAATGGTGAACTCTTCGATTTAAGTGCGGGTGCTGTGGGCTTTGCGATTGGCGCTGAATACCGT
CGTGAAAGTGGTTTCTATAATCCAGATCCAGTTGTTGTCGCTGGCGATAGTACTGCTGCTCAGCAAGACCCTACAAGCGG
TAACTACAACGTTATCTCTATTTTCCAAGAAGTGAGCGTACCATTTACAGAAAAATTAACGGGTGAATTTGCACTGCGTT
TAGATGATTATTCAACATTTGGTAAAGCAACTACGTGGAAAATTGGGTTGACATATGTCGCATCCGACGAACTTATGTTA
CGTACTGTCGCTGCTACAGGTTTCCGTGCTCCAAGTGTTGCTGAATTATATGGTGGTAATTCAGGTTCTTTTGATTATTT
AACGAACCCTTGGTTACCTCTGAGTGAACAAACTGGACAGATTTTAGTTAATCGCACATCTGATGAAAATTTAAAACCTG
AAGAATCTGAGTCATATACCGCTGGTTTAGTTTACTCACCAAGCTATATTGATGGTATGTCTCTGACTTTAGACTACTGG
CGTTTCAAAGTAACCAATGCTATTGCTCGTGCAGATGTTCAAGTTGGTTTAGATGACTGTTATAAAGGTGATGCTGAAGC
ATGTAGTACATTCAATATTACTGCCGATGGTGATTTGTTTAATTTAACAAGTGCATTAACAAACGTTGGGTCACAGGATA
CTAGCGGTGTTGATTTCAACTTAGCTTATAGCTTTGCATTGTTTGGGTTAGACTGGAAAGTCAGTAATGACACTACATAC
CTCATTAATTATGAGCAAGATGGTGAAAACTATACTGGCACTATCGATGGGAACTTCGGTGCTTACTCTCGTGTTCGTAA
CAACTTCAGCATTTCTGCGGGTCAAGATGATTGGAGTGTAATGTACTTCAACCGCTATATCCGATCTATGAATGATAATT
ACACTGACTATGATGTGAATGATGAACCATTTGCTGCTGTTAGAGGTGTTGATTCTATTCTGTATCACAATATTTCTGGT
ACTTATCACGTAAATGACAGCACCACAGTTAGCTTAGGGGTGAAGAACTTTACTGACGAGAAGCCGCTATACGTTACTAA
CGGTAGTGATGCTGGTACAGTTCCAGAAGTGTACGACACCATTGGCCGTACTATATACGGTGGTGTAACGGTTAAGTTCT
AA

Upstream 100 bases:

>100_bases
GTTAAAGTGAATCATCAGTATCGAGTGCAACACTAGTACATCAATAGTTGTACAAGCTGTAAGCATAAAAATAATAGTAA
GATCTAGGGAGCATTCATAC

Downstream 100 bases:

>100_bases
GCTAAGTTAATACTCTTAGTGTTTAAAAAGCGCCTTAGGGTAATGCCGTTCACTTAAGAGTGAACGGCATTTTTCTTTGG
TCTAACCGGATACTTGTTTT

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MIKRTKVASAINLAIVASITSGAFVTSSAFAAEEKAKVERIEVTGSRIQRQDMETASPVTVIDAAAIKAEGFTSVDQMLQ
VQTSMAGAAVGSSTNNGADGVAQVDLRGMGSQRTLVLLNGRRMVNSGSGADSAVDLNSIPVAMIARVEILKDGASAVYGS
DAIAGVVNIITKKDFEGFQLDFNGSGTDKGDGESGDVSALYGFNTEGGNYTFGAAYADRRGVIQGDRDWYDPLDNTSSII
PTGALNQKVIDANGNWVNRATGYDYSNDVYFQTPSERRSLFANMTQELGNDVVLTADAIYTNRRSNQQLAAQPAQIKLNV
CGDPRVTDPTAGCLNLDQSMVDAGIKADARGQVQYNRRMNDVGPRVYSQDTDTWRLSGGLAGSLDVHTGMNWNVDYTFGK
NEAKTGVDNSINATLMAASIYADPYAWFEGSPLTNEMKDVSYDEKSTGGNEQQTLSAGLNGELFDLSAGAVGFAIGAEYR
RESGFYNPDPVVVAGDSTAAQQDPTSGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGKATTWKIGLTYVASDELML
RTVAATGFRAPSVAELYGGNSGSFDYLTNPWLPLSEQTGQILVNRTSDENLKPEESESYTAGLVYSPSYIDGMSLTLDYW
RFKVTNAIARADVQVGLDDCYKGDAEACSTFNITADGDLFNLTSALTNVGSQDTSGVDFNLAYSFALFGLDWKVSNDTTY
LINYEQDGENYTGTIDGNFGAYSRVRNNFSISAGQDDWSVMYFNRYIRSMNDNYTDYDVNDEPFAAVRGVDSILYHNISG
TYHVNDSTTVSLGVKNFTDEKPLYVTNGSDAGTVPEVYDTIGRTIYGGVTVKF

Sequences:

>Translated_853_residues
MIKRTKVASAINLAIVASITSGAFVTSSAFAAEEKAKVERIEVTGSRIQRQDMETASPVTVIDAAAIKAEGFTSVDQMLQ
VQTSMAGAAVGSSTNNGADGVAQVDLRGMGSQRTLVLLNGRRMVNSGSGADSAVDLNSIPVAMIARVEILKDGASAVYGS
DAIAGVVNIITKKDFEGFQLDFNGSGTDKGDGESGDVSALYGFNTEGGNYTFGAAYADRRGVIQGDRDWYDPLDNTSSII
PTGALNQKVIDANGNWVNRATGYDYSNDVYFQTPSERRSLFANMTQELGNDVVLTADAIYTNRRSNQQLAAQPAQIKLNV
CGDPRVTDPTAGCLNLDQSMVDAGIKADARGQVQYNRRMNDVGPRVYSQDTDTWRLSGGLAGSLDVHTGMNWNVDYTFGK
NEAKTGVDNSINATLMAASIYADPYAWFEGSPLTNEMKDVSYDEKSTGGNEQQTLSAGLNGELFDLSAGAVGFAIGAEYR
RESGFYNPDPVVVAGDSTAAQQDPTSGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGKATTWKIGLTYVASDELML
RTVAATGFRAPSVAELYGGNSGSFDYLTNPWLPLSEQTGQILVNRTSDENLKPEESESYTAGLVYSPSYIDGMSLTLDYW
RFKVTNAIARADVQVGLDDCYKGDAEACSTFNITADGDLFNLTSALTNVGSQDTSGVDFNLAYSFALFGLDWKVSNDTTY
LINYEQDGENYTGTIDGNFGAYSRVRNNFSISAGQDDWSVMYFNRYIRSMNDNYTDYDVNDEPFAAVRGVDSILYHNISG
TYHVNDSTTVSLGVKNFTDEKPLYVTNGSDAGTVPEVYDTIGRTIYGGVTVKF
>Mature_853_residues
MIKRTKVASAINLAIVASITSGAFVTSSAFAAEEKAKVERIEVTGSRIQRQDMETASPVTVIDAAAIKAEGFTSVDQMLQ
VQTSMAGAAVGSSTNNGADGVAQVDLRGMGSQRTLVLLNGRRMVNSGSGADSAVDLNSIPVAMIARVEILKDGASAVYGS
DAIAGVVNIITKKDFEGFQLDFNGSGTDKGDGESGDVSALYGFNTEGGNYTFGAAYADRRGVIQGDRDWYDPLDNTSSII
PTGALNQKVIDANGNWVNRATGYDYSNDVYFQTPSERRSLFANMTQELGNDVVLTADAIYTNRRSNQQLAAQPAQIKLNV
CGDPRVTDPTAGCLNLDQSMVDAGIKADARGQVQYNRRMNDVGPRVYSQDTDTWRLSGGLAGSLDVHTGMNWNVDYTFGK
NEAKTGVDNSINATLMAASIYADPYAWFEGSPLTNEMKDVSYDEKSTGGNEQQTLSAGLNGELFDLSAGAVGFAIGAEYR
RESGFYNPDPVVVAGDSTAAQQDPTSGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTFGKATTWKIGLTYVASDELML
RTVAATGFRAPSVAELYGGNSGSFDYLTNPWLPLSEQTGQILVNRTSDENLKPEESESYTAGLVYSPSYIDGMSLTLDYW
RFKVTNAIARADVQVGLDDCYKGDAEACSTFNITADGDLFNLTSALTNVGSQDTSGVDFNLAYSFALFGLDWKVSNDTTY
LINYEQDGENYTGTIDGNFGAYSRVRNNFSISAGQDDWSVMYFNRYIRSMNDNYTDYDVNDEPFAAVRGVDSILYHNISG
TYHVNDSTTVSLGVKNFTDEKPLYVTNGSDAGTVPEVYDTIGRTIYGGVTVKF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=164, Percent_Identity=31.7073170731707, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788478, Length=137, Percent_Identity=33.5766423357664, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786798, Length=102, Percent_Identity=37.2549019607843, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 92009; Mature: 92009

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRTKVASAINLAIVASITSGAFVTSSAFAAEEKAKVERIEVTGSRIQRQDMETASPVT
CCCCHHHHHHEEEEEEEEECCCCEEECHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCEE
VIDAAAIKAEGFTSVDQMLQVQTSMAGAAVGSSTNNGADGVAQVDLRGMGSQRTLVLLNG
EEEEEHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEC
RRMVNSGSGADSAVDLNSIPVAMIARVEILKDGASAVYGSDAIAGVVNIITKKDFEGFQL
CEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEEE
DFNGSGTDKGDGESGDVSALYGFNTEGGNYTFGAAYADRRGVIQGDRDWYDPLDNTSSII
EECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
PTGALNQKVIDANGNWVNRATGYDYSNDVYFQTPSERRSLFANMTQELGNDVVLTADAIY
CCCCCCCEEEECCCCEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEHHEE
TNRRSNQQLAAQPAQIKLNVCGDPRVTDPTAGCLNLDQSMVDAGIKADARGQVQYNRRMN
ECCCCCHHHHCCCCEEEEEECCCCCCCCCCCCHHCCCHHHHHCCCCCCCCCCEEECCCCH
DVGPRVYSQDTDTWRLSGGLAGSLDVHTGMNWNVDYTFGKNEAKTGVDNSINATLMAASI
HCCCEEECCCCCEEEECCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEE
YADPYAWFEGSPLTNEMKDVSYDEKSTGGNEQQTLSAGLNGELFDLSAGAVGFAIGAEYR
ECCCCCEECCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEEECCCHHH
RESGFYNPDPVVVAGDSTAAQQDPTSGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTF
HHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHCCCEEEEEECCCCCC
GKATTWKIGLTYVASDELMLRTVAATGFRAPSVAELYGGNSGSFDYLTNPWLPLSEQTGQ
CCCEEEEEEEEEEECCCEEEEEHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCE
ILVNRTSDENLKPEESESYTAGLVYSPSYIDGMSLTLDYWRFKVTNAIARADVQVGLDDC
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHEEEHHHHHHHHHEEECHHHH
YKGDAEACSTFNITADGDLFNLTSALTNVGSQDTSGVDFNLAYSFALFGLDWKVSNDTTY
CCCCCHHCEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEECCCEEE
LINYEQDGENYTGTIDGNFGAYSRVRNNFSISAGQDDWSVMYFNRYIRSMNDNYTDYDVN
EEEEECCCCCEEEEECCCCCCHHHHCCCEEEECCCCCCEEHHHHHHHHHCCCCCCCCCCC
DEPFAAVRGVDSILYHNISGTYHVNDSTTVSLGVKNFTDEKPLYVTNGSDAGTVPEVYDT
CCCHHHHHHHHHHHEECCCCEEEECCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHH
IGRTIYGGVTVKF
HCCEEECCEEEEC
>Mature Secondary Structure
MIKRTKVASAINLAIVASITSGAFVTSSAFAAEEKAKVERIEVTGSRIQRQDMETASPVT
CCCCHHHHHHEEEEEEEEECCCCEEECHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCEE
VIDAAAIKAEGFTSVDQMLQVQTSMAGAAVGSSTNNGADGVAQVDLRGMGSQRTLVLLNG
EEEEEHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEC
RRMVNSGSGADSAVDLNSIPVAMIARVEILKDGASAVYGSDAIAGVVNIITKKDFEGFQL
CEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEEE
DFNGSGTDKGDGESGDVSALYGFNTEGGNYTFGAAYADRRGVIQGDRDWYDPLDNTSSII
EECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
PTGALNQKVIDANGNWVNRATGYDYSNDVYFQTPSERRSLFANMTQELGNDVVLTADAIY
CCCCCCCEEEECCCCEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEHHEE
TNRRSNQQLAAQPAQIKLNVCGDPRVTDPTAGCLNLDQSMVDAGIKADARGQVQYNRRMN
ECCCCCHHHHCCCCEEEEEECCCCCCCCCCCCHHCCCHHHHHCCCCCCCCCCEEECCCCH
DVGPRVYSQDTDTWRLSGGLAGSLDVHTGMNWNVDYTFGKNEAKTGVDNSINATLMAASI
HCCCEEECCCCCEEEECCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEE
YADPYAWFEGSPLTNEMKDVSYDEKSTGGNEQQTLSAGLNGELFDLSAGAVGFAIGAEYR
ECCCCCEECCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEEECCCHHH
RESGFYNPDPVVVAGDSTAAQQDPTSGNYNVISIFQEVSVPFTEKLTGEFALRLDDYSTF
HHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHCCCEEEEEECCCCCC
GKATTWKIGLTYVASDELMLRTVAATGFRAPSVAELYGGNSGSFDYLTNPWLPLSEQTGQ
CCCEEEEEEEEEEECCCEEEEEHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCE
ILVNRTSDENLKPEESESYTAGLVYSPSYIDGMSLTLDYWRFKVTNAIARADVQVGLDDC
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHEEEHHHHHHHHHEEECHHHH
YKGDAEACSTFNITADGDLFNLTSALTNVGSQDTSGVDFNLAYSFALFGLDWKVSNDTTY
CCCCCHHCEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEECCCEEE
LINYEQDGENYTGTIDGNFGAYSRVRNNFSISAGQDDWSVMYFNRYIRSMNDNYTDYDVN
EEEEECCCCCEEEEECCCCCCHHHHCCCEEEECCCCCCEEHHHHHHHHHCCCCCCCCCCC
DEPFAAVRGVDSILYHNISGTYHVNDSTTVSLGVKNFTDEKPLYVTNGSDAGTVPEVYDT
CCCHHHHHHHHHHHEECCCCEEEECCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHH
IGRTIYGGVTVKF
HCCEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11598060 [H]