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The map label for this gene is maiA [H]

Identifier: 160874636

GI number: 160874636

Start: 1831045

End: 1831695

Strand: Direct

Name: maiA [H]

Synonym: Sbal195_1519

Alternate gene names: 160874636

Gene position: 1831045-1831695 (Clockwise)

Preceding gene: 160874635

Following gene: 160874637

Centisome position: 34.24

GC content: 50.54

Gene sequence:

>651_bases
ATGAAACTTTATGGTTATTGGCGCTCAAGCGCGGCGTACAGAGTTCGCATCGCCCTCAATCTTAAGGGGATTTCAGCCGA
GCAGTTGTCTGTGCATCTTGTACGTGATGGCGGCGAGCAACATAAAGCGGCCTACAGTGCGCTTAATCCGCTGGAGTTAG
TGCCAACCTTAACGTTGGATGATGAGCTGGATGCGGATGCATTGTCGCAATCCTTAGCCATTATCGAATATCTTGATGAG
ATACACCCTCAGTCACCATTATTGCCTGCATCGGCTCTTGAGCGTGCCCATGTGCGCGCCATGGCACTCACGGTAGCCTG
TGAAATTCACCCATTAAACAATTTGCGGGTATTGCAGTATTTAACCCAAACCTTAGGGGTCGATGAAGCGGCTAAAAATA
CTTGGTATCACCACTGGGTCGCGAGTGGCTTTGCAGCCCTAGAAACCTTGTTGGTAAGGCACAGCGGTCGTTACTGCTTT
GGGGATACAGTGACATTAGCCGACTTATGTTTAGTGCCGCAGGTGTATAACGCCCAGCGTTTTAATGTGGATTTAACGCC
TTATCCCAATATCATGCGCGTGTGGACTGAGTGTAATCAGCTAGAAGCCTTTGCCGATGCGGCCCCAGAGCGCCAAGCGG
ATGCGGTGTGA

Upstream 100 bases:

>100_bases
ATACAAAGCCTAAGGCTTTCCCTTAAAGGGCTACATCGACTCAAACTAACGGGGCTTAATTGCCCCGTTTTTATACTGAC
TTTTATAAGGATGTGAAAAT

Downstream 100 bases:

>100_bases
TGCTTTGTAACGAATAGTGCGGATATAGTGTTTACCAATTGAAAGAATGGATGATGAGGCTTGTTTTGCGAGTCTTATCT
CAGCGATGGAGTTTAAATGC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLTLDDELDADALSQSLAIIEYLDE
IHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQYLTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCF
GDTVTLADLCLVPQVYNAQRFNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV

Sequences:

>Translated_216_residues
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLTLDDELDADALSQSLAIIEYLDE
IHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQYLTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCF
GDTVTLADLCLVPQVYNAQRFNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV
>Mature_216_residues
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLTLDDELDADALSQSLAIIEYLDE
IHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQYLTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCF
GDTVTLADLCLVPQVYNAQRFNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=48.5849056603774, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=39.1509433962264, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI22202622, Length=165, Percent_Identity=46.6666666666667, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17551302, Length=212, Percent_Identity=44.3396226415094, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17510461, Length=213, Percent_Identity=40.3755868544601, Blast_Score=156, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17556142, Length=214, Percent_Identity=40.1869158878505, Blast_Score=146, Evalue=7e-36,
Organism=Drosophila melanogaster, GI21355859, Length=213, Percent_Identity=45.5399061032864, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24645375, Length=213, Percent_Identity=45.5399061032864, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI45553325, Length=213, Percent_Identity=45.5399061032864, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI21355857, Length=213, Percent_Identity=38.9671361502347, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24087; Mature: 24087

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLTLD
CCEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHCEEEECC
DELDADALSQSLAIIEYLDEIHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHH
LTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCFGDTVTLADLCLVPQVYNAQR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCHHCCCEE
FNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLTLD
CCEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHCEEEECC
DELDADALSQSLAIIEYLDEIHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCHHHHHH
LTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCFGDTVTLADLCLVPQVYNAQR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCHHCCCEE
FNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]