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The map label for this gene is anfA [H]

Identifier: 160874561

GI number: 160874561

Start: 1732204

End: 1733574

Strand: Direct

Name: anfA [H]

Synonym: Sbal195_1443

Alternate gene names: 160874561

Gene position: 1732204-1733574 (Clockwise)

Preceding gene: 160874558

Following gene: 160874562

Centisome position: 32.39

GC content: 49.96

Gene sequence:

>1371_bases
ATGGCTCGATTGACACTTTTTTATCACCTGCAGGATGTAAACGCCGAACAAGCCATCTTAGAACTCGCCGGCGCTGCCGA
CTTTAATAAGCTACGCAGTGTCGATGATTTACCTTGGATAGAACAGCTTGAGGCGAATCACGTCGATGTTGCCATCATTG
AGCTGTCCAGCCTTAGCCGTGAGGATTATCTCGGGCTACTGGACAATGTAGCTCTGTCCAATATCGAGTTTATTTTTCTC
TCCGACGGCCAGCCGAACCCCAATCTTGATCAGTTAATGTCGCATTTTGCCGGTTATCATTTTCGCAAACCCTACGACAT
GACGTTAATCAGCGATATTTTCGCCGATTTTGCCCAGTATCTAGTGTCATCCCCAATTCGGCCTCGGCCTTTTTCCAGCG
AGCTAGACCAATATGGGTTACTCGTCGGCTCCTCTGCTGCGATGCATAAGCTGTATCGCACGATTCGCCGCGTCTCCGCG
ACCGACAGTAATGTGCTGATCATTGGTGAGAGTGGGGCGGGGAAAGAGCTGGTCGCCAATACCATACATCTTGCCAGCCC
TAGGCTAAACGAGCCTTTTATCGCGATTAACTGCGGCGCCTTAAGCCCTGAATTGGTCGATAGCGAGTTGTTTGGCCACG
TAAAAGGCGCGTTCACTGGCGCTCATCGCGATCACAAAGGCGTATTTGAGCAGGCAGAAGGCGGAACCTTATTTCTCGAT
GAAGTCACCGAAATGCCAATTGAGCATCAAGTGAAGTTACTCAGAGTGCTTGAAAATAGCGAATATCGCCCTGTTGGCGG
TGCCAAAGTGCAACTCGCCAATGTGCGGATTGTGGCGGCCACCAATCGTGATCCCATAGCGGCAATCGAGGCTGGGCAAT
TTCGCGAGGATTTATATTTCCGTTTGGCCCAGTTCCCTATCCGCGTTCCGCCATTAAGGGAACGTGGCGATGATATTCTT
GGGTTGGCGCAGCATTTTCTTGCCTATCGTAACGTGGCAGAAAAGCAGAATAAAAGCCTCGCTGATGACAGTGCGCAGAT
GATCGCTGCGTACCCTTGGTCGGGTAATGTGCGTGAATTAAAGCATGCCATTGAGCGAGCCTATATTCTGGCAGACCAAG
AGATTTTACCCAGCCATTTACAGTTGCCACAGGCGAGTGAAATGGGAACATCTTGCGATGACCAAGTCGTTATCCCGCAT
GGCATGCGTTTAGATGACTTAGAAAAAATCGCCATTTATCAAGCATTAGAAACAACTCAAGGCAATAAAACCGATACCGC
CGAGCAGCTGGGGATCAGCGTGAAAACTCTCTACAACAAGCTCAGCAAGTACGAGCAGCAAGTCGAAGCGGGTGAATCGA
CGGTGGAATAG

Upstream 100 bases:

>100_bases
TACGCCATCCTCTTGGAATATCGGCAACTATTACCGCTGCTGTTGTTAAAATTTCACGACTGACATACAGTAGCCCTTCA
TACACAGGGAGTGGGATAGC

Downstream 100 bases:

>100_bases
TTTTTTGCGTTCAAAAGCCGCAGATGTGAGTGAGTTTAACATCTCATTGAGCTTTATCCTGTGGCTTTAGCCATCTTAAG
CATACCGTTGATGTGGTTGC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSREDYLGLLDNVALSNIEFIFL
SDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQYLVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSA
TDSNVLIIGESGAGKELVANTIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYFRLAQFPIRVPPLRERGDDIL
GLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVRELKHAIERAYILADQEILPSHLQLPQASEMGTSCDDQVVIPH
GMRLDDLEKIAIYQALETTQGNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESTVE

Sequences:

>Translated_456_residues
MARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSREDYLGLLDNVALSNIEFIFL
SDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQYLVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSA
TDSNVLIIGESGAGKELVANTIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYFRLAQFPIRVPPLRERGDDIL
GLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVRELKHAIERAYILADQEILPSHLQLPQASEMGTSCDDQVVIPH
GMRLDDLEKIAIYQALETTQGNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESTVE
>Mature_455_residues
ARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSREDYLGLLDNVALSNIEFIFLS
DGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQYLVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSAT
DSNVLIIGESGAGKELVANTIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLDE
VTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYFRLAQFPIRVPPLRERGDDILG
LAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVRELKHAIERAYILADQEILPSHLQLPQASEMGTSCDDQVVIPHG
MRLDDLEKIAIYQALETTQGNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESTVE

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=315, Percent_Identity=45.0793650793651, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788550, Length=315, Percent_Identity=42.2222222222222, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=41.0658307210031, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=40.7407407407407, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788905, Length=309, Percent_Identity=42.7184466019417, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=41.85303514377, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1790299, Length=329, Percent_Identity=39.209726443769, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=39.3650793650794, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI87081872, Length=317, Percent_Identity=37.8548895899054, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=37.6543209876543, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=32.4921135646688, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=34.0807174887892, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=28.6184210526316, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50713; Mature: 50582

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSR
CCEEEEEEEEECCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCCH
EDYLGLLDNVALSNIEFIFLSDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQY
HHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSATDSNVLIIGESGAGKELVAN
HHCCCCCCCCCHHHHHHHCEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
TIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEC
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYF
HHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEECCHHHHHHHHH
RLAQFPIRVPPLRERGDDILGLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVREL
HHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHH
KHAIERAYILADQEILPSHLQLPQASEMGTSCDDQVVIPHGMRLDDLEKIAIYQALETTQ
HHHHHHHHHEECHHHCHHHHCCCCHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCC
GNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESTVE
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSR
CEEEEEEEEECCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCCH
EDYLGLLDNVALSNIEFIFLSDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQY
HHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSATDSNVLIIGESGAGKELVAN
HHCCCCCCCCCHHHHHHHCEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
TIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEC
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYF
HHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEECCHHHHHHHHH
RLAQFPIRVPPLRERGDDILGLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVREL
HHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHH
KHAIERAYILADQEILPSHLQLPQASEMGTSCDDQVVIPHGMRLDDLEKIAIYQALETTQ
HHHHHHHHHEECHHHCHHHHCCCCHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCC
GNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESTVE
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]