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The map label for this gene is rsmB [H]

Identifier: 160874533

GI number: 160874533

Start: 1698608

End: 1699969

Strand: Reverse

Name: rsmB [H]

Synonym: Sbal195_1415

Alternate gene names: 160874533

Gene position: 1699969-1698608 (Counterclockwise)

Preceding gene: 160874534

Following gene: 160874532

Centisome position: 31.79

GC content: 49.56

Gene sequence:

>1362_bases
ATGGCATCTAACTCAGTCACATCATCGGCGTTAAATGTTCAGGCGAACACCCCAGCGCAAAAACGTGCGCTCAGTTACGC
CAGTACAATTAAGCAATTATTCGATGAAATTCAAAGCAGTAAAATGCCCGCCGACCGTATTCTGGCAAACTACTTTAGAG
AACATAAAAAACATGGCTCGAAGGATCGTAAAGTCATTCGTGAATCCTTGTTTAGTCTATTTCGCTGGTGGGGTTGGTTT
CAGCGATTAGGCGATCAGCAAGAATCTGCCGTTTATTTTGCTAGCCTAAGCGCCAGTGCTCTATTGGAAGCCCATACTTG
GCAAGATATCGCCAACGCTTGGCATGAGTTATCACAAACGCCCATCGATTATCAGCAAGCTCAAGCACTTGCCGTTGAAA
GTCTCGACGACAAACTGGCACTGATACAGTATTTATTCCCTGCCACTGAGTTTAAGGCACAGGATTTATTACCCGACTGG
TTCTGGCAAGTGTGCCCTGTTGATGCAGCGCAACAAACGCAATTAATTGCAGCTATGTCCTGTCGCCCGCCAATTTGGGG
ACGCGCGCAAGGGATCACGAGTCAAGACGCAGCGACACAACTGCAAGCGCTCGGTATAGAAGCGAGTTATGGTCATTACT
TTAGCGATGCCATCAATCTTGGCAGTAAGAGCATGAATCTTAATGAAATAGCCCTGTATAAAGACGGTAACATCGAGATC
CAAGATTTGGCTTCACAGGTCATAGGGCAAATTTGCGCGCCTGAACCCGATGCCCATTGGTGGGATGCTTGTAGCGGTGC
TGGCGGCAAAACCCTGCAACTGCGCTCTTTGATGTTAGCTAAAGCTGCTGATAACCAAACGTTATCTGCGGGCAGCATCA
TATCGTCCGATATTCGCTCCAATGCCCTAGAAGAACTCACTAAACGCGCCCAAAGAGCCAACTTTAACGGCATTAGCGTG
GCCCGTTGGAAAAGTGATGCCCTCCCCGTGCCTGCGGATCATTTTGACGGTGTGTTAGTCGATGCCCCGTGCAGTTGTAC
TGGCACTTGGCGACGTAATCCTGATATGCGTTGGTTGGATGGTGTTGAAGCCGTGGCCGATAAAGCCGCGTTACAGCTCG
ATATTTTAAGCCGCAGTAGCCGCGCAGTAAAGGCCGGTGGCATCCTCGTTTACGCCACTTGCTCCTTGTCGCCCATCGAG
AACCAAGACGTGGTAACGGCGTTTTTACAGCAACATCCAGAGTTCACGCTCACGCCGATACGCCATCCTTTTAGCGGTGA
ACAAACAGAAATGTTGACCGTGTTGCCCTTTGAGGCCAACAGTGATGGCATGTTCGTCGCACGTATGCTGCGCAGCAAGT
AA

Upstream 100 bases:

>100_bases
TTTTTCCCCTCTGTTCAGCTATGCCTCTTACATAAGGCGCAGTCACAGAAGCATGATCTAGGCTAAATTTATGGGTAATC
CAAGCAGCTTCTTTTCCGGT

Downstream 100 bases:

>100_bases
CACTAACTCATTATTTTGTATCAAAACTTGGCGCCCTCCGTTCTGGGCGCCAAGTTCAATCGTATACTGTGTCTTACGAT
TAAAATCAGATTTACTCTTT

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MASNSVTSSALNVQANTPAQKRALSYASTIKQLFDEIQSSKMPADRILANYFREHKKHGSKDRKVIRESLFSLFRWWGWF
QRLGDQQESAVYFASLSASALLEAHTWQDIANAWHELSQTPIDYQQAQALAVESLDDKLALIQYLFPATEFKAQDLLPDW
FWQVCPVDAAQQTQLIAAMSCRPPIWGRAQGITSQDAATQLQALGIEASYGHYFSDAINLGSKSMNLNEIALYKDGNIEI
QDLASQVIGQICAPEPDAHWWDACSGAGGKTLQLRSLMLAKAADNQTLSAGSIISSDIRSNALEELTKRAQRANFNGISV
ARWKSDALPVPADHFDGVLVDAPCSCTGTWRRNPDMRWLDGVEAVADKAALQLDILSRSSRAVKAGGILVYATCSLSPIE
NQDVVTAFLQQHPEFTLTPIRHPFSGEQTEMLTVLPFEANSDGMFVARMLRSK

Sequences:

>Translated_453_residues
MASNSVTSSALNVQANTPAQKRALSYASTIKQLFDEIQSSKMPADRILANYFREHKKHGSKDRKVIRESLFSLFRWWGWF
QRLGDQQESAVYFASLSASALLEAHTWQDIANAWHELSQTPIDYQQAQALAVESLDDKLALIQYLFPATEFKAQDLLPDW
FWQVCPVDAAQQTQLIAAMSCRPPIWGRAQGITSQDAATQLQALGIEASYGHYFSDAINLGSKSMNLNEIALYKDGNIEI
QDLASQVIGQICAPEPDAHWWDACSGAGGKTLQLRSLMLAKAADNQTLSAGSIISSDIRSNALEELTKRAQRANFNGISV
ARWKSDALPVPADHFDGVLVDAPCSCTGTWRRNPDMRWLDGVEAVADKAALQLDILSRSSRAVKAGGILVYATCSLSPIE
NQDVVTAFLQQHPEFTLTPIRHPFSGEQTEMLTVLPFEANSDGMFVARMLRSK
>Mature_452_residues
ASNSVTSSALNVQANTPAQKRALSYASTIKQLFDEIQSSKMPADRILANYFREHKKHGSKDRKVIRESLFSLFRWWGWFQ
RLGDQQESAVYFASLSASALLEAHTWQDIANAWHELSQTPIDYQQAQALAVESLDDKLALIQYLFPATEFKAQDLLPDWF
WQVCPVDAAQQTQLIAAMSCRPPIWGRAQGITSQDAATQLQALGIEASYGHYFSDAINLGSKSMNLNEIALYKDGNIEIQ
DLASQVIGQICAPEPDAHWWDACSGAGGKTLQLRSLMLAKAADNQTLSAGSIISSDIRSNALEELTKRAQRANFNGISVA
RWKSDALPVPADHFDGVLVDAPCSCTGTWRRNPDMRWLDGVEAVADKAALQLDILSRSSRAVKAGGILVYATCSLSPIEN
QDVVTAFLQQHPEFTLTPIRHPFSGEQTEMLTVLPFEANSDGMFVARMLRSK

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI39995082, Length=189, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI301336155, Length=189, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI32698918, Length=201, Percent_Identity=27.8606965174129, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI76150625, Length=245, Percent_Identity=25.3061224489796, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI76150623, Length=245, Percent_Identity=25.3061224489796, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI11545785, Length=210, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI2367212, Length=310, Percent_Identity=30.3225806451613, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87081985, Length=259, Percent_Identity=25.4826254826255, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25143471, Length=187, Percent_Identity=29.9465240641711, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536757, Length=236, Percent_Identity=25, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319447, Length=188, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355201, Length=186, Percent_Identity=30.6451612903226, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920494, Length=166, Percent_Identity=33.1325301204819, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 50070; Mature: 49939

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNSVTSSALNVQANTPAQKRALSYASTIKQLFDEIQSSKMPADRILANYFREHKKHGS
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KDRKVIRESLFSLFRWWGWFQRLGDQQESAVYFASLSASALLEAHTWQDIANAWHELSQT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
PIDYQQAQALAVESLDDKLALIQYLFPATEFKAQDLLPDWFWQVCPVDAAQQTQLIAAMS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHC
CRPPIWGRAQGITSQDAATQLQALGIEASYGHYFSDAINLGSKSMNLNEIALYKDGNIEI
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEH
QDLASQVIGQICAPEPDAHWWDACSGAGGKTLQLRSLMLAKAADNQTLSAGSIISSDIRS
HHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NALEELTKRAQRANFNGISVARWKSDALPVPADHFDGVLVDAPCSCTGTWRRNPDMRWLD
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCEEEECCCCCCCCCCCCCCCHHHH
GVEAVADKAALQLDILSRSSRAVKAGGILVYATCSLSPIENQDVVTAFLQQHPEFTLTPI
HHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCC
RHPFSGEQTEMLTVLPFEANSDGMFVARMLRSK
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
ASNSVTSSALNVQANTPAQKRALSYASTIKQLFDEIQSSKMPADRILANYFREHKKHGS
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KDRKVIRESLFSLFRWWGWFQRLGDQQESAVYFASLSASALLEAHTWQDIANAWHELSQT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
PIDYQQAQALAVESLDDKLALIQYLFPATEFKAQDLLPDWFWQVCPVDAAQQTQLIAAMS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHC
CRPPIWGRAQGITSQDAATQLQALGIEASYGHYFSDAINLGSKSMNLNEIALYKDGNIEI
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEH
QDLASQVIGQICAPEPDAHWWDACSGAGGKTLQLRSLMLAKAADNQTLSAGSIISSDIRS
HHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NALEELTKRAQRANFNGISVARWKSDALPVPADHFDGVLVDAPCSCTGTWRRNPDMRWLD
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCEEEECCCCCCCCCCCCCCCHHHH
GVEAVADKAALQLDILSRSSRAVKAGGILVYATCSLSPIENQDVVTAFLQQHPEFTLTPI
HHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCC
RHPFSGEQTEMLTVLPFEANSDGMFVARMLRSK
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA