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The map label for this gene is insF7 [C]

Identifier: 160874498

GI number: 160874498

Start: 1653730

End: 1654497

Strand: Reverse

Name: insF7 [C]

Synonym: Sbal195_1380

Alternate gene names: 160874498

Gene position: 1654497-1653730 (Counterclockwise)

Preceding gene: 160874499

Following gene: 160874496

Centisome position: 30.94

GC content: 46.88

Gene sequence:

>768_bases
ATGGCGAGCCGAAGCACTGAGCTATCGGTCATCAAAGACTGGGTCCAATATTGGCGTAACTATATGCCACGATTGGGGAC
GCGTAAGCTCTATACTTTGATACAGCCTAAGCTGGTTGAACATGATATCAAACTCGGGCGAGACGGGTTCTTTACCTACT
TGAGAAGTGAAGGCTTGCTAGTTAAGCCCAAGAGAAGCTTCACAAAAACGACATTCAGCAAGCATTGGATGAAGAAGCAT
CCCAACCTGCTGAAGGAAGAGGGACTACACGATGCCGAGCATGTACTGGTCAGCGATATCACTTATCTCGAATCAGACCA
AGGCGTGCACTATTTGTCACTAGTGACCGATGCCGCGACACGTAAGATAGTCGGTCATCACTTGAGTTCGGACATGAAAG
CAGAAAGCGTAGTGAAAGCACTAAAAATGGCGGTCAAAGATAAGCGCTATAGCGCTAATGCGATCCATCACTCAGACCGA
GGTTCGCAATACTGCTCAGCTGTTTATCAAGATGAACTGCAGGCCAACCATATTCAGCCGTCGATGACAGACGGTTATGA
TTGCTATCAAAATGCGTTAGCAGAACGAGTAAACGGGATCCTGAAGCAGGAGTTTTTACTCTACCGATGTAAAACGCTGG
AAGAGCTGAAGAGACTTGTTAGAGAATCGATAGCGATATACAACGAAATGAGACCGCACCTGAGTTTGGATATGGAAACA
CCCAATCAGGTGCATAATAGAAAAGGCCAGCTACTGGAGCTGGCCTAG

Upstream 100 bases:

>100_bases
GTATCTCTGGCAAGCCAAAACCCAAAGCAAAATAAACCTTGCCGTTGCGTGTCGCGCTGTCGGTATTTCACGGCAAGGTA
TTTATCAGGCTGTCGCCCGA

Downstream 100 bases:

>100_bases
AAACTGTCAACCTATCTTAGGACGGGACAGTCTGTTATAACAGCGGCTGATCCAATAAAGCTTAAGCTTCGATATCTATC
GTTGCGCTTTCTTGGTTAGG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MASRSTELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLLVKPKRSFTKTTFSKHWMKKH
PNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAATRKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAIHHSDR
GSQYCSAVYQDELQANHIQPSMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMET
PNQVHNRKGQLLELA

Sequences:

>Translated_255_residues
MASRSTELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLLVKPKRSFTKTTFSKHWMKKH
PNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAATRKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAIHHSDR
GSQYCSAVYQDELQANHIQPSMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMET
PNQVHNRKGQLLELA
>Mature_254_residues
ASRSTELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLLVKPKRSFTKTTFSKHWMKKHP
NLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAATRKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAIHHSDRG
SQYCSAVYQDELQANHIQPSMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMETP
NQVHNRKGQLLELA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=194, Percent_Identity=32.4742268041237, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788405, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787263, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786753, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786570, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786490, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789981, Length=152, Percent_Identity=32.8947368421053, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29577; Mature: 29446

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHCCCCCEEECC
>Mature Secondary Structure 
ASRSTELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCE
VKPKRSFTKTTFSKHWMKKHPNLLKEEGLHDAEHVLVSDITYLESDQGVHYLSLVTDAAT
ECCCHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAIHHSDRGSQYCSAVYQDELQANHIQP
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCC
SMTDGYDCYQNALAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMET
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
PNQVHNRKGQLLELA
CHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]