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The map label for this gene is oprF [H]

Identifier: 160874266

GI number: 160874266

Start: 1357859

End: 1358968

Strand: Reverse

Name: oprF [H]

Synonym: Sbal195_1146

Alternate gene names: 160874266

Gene position: 1358968-1357859 (Counterclockwise)

Preceding gene: 160874267

Following gene: 160874265

Centisome position: 25.41

GC content: 43.6

Gene sequence:

>1110_bases
ATGGAATTGGAGAACATGATGAATCATAAATTAAAATTTGTACTGCTTGCATCGCTATTACCTTTCACTGTATCTGCGGC
TCAAGAACTGACTCCTTGGTATGTTGGCGCAGGTTTAGGCGTTAACAACTATGAACATATCGCGACCCAGAATGGTCAAG
ATGACCCATATGCATGGGATGTATTCGCAGGCTATATGTTCAATGATTACTTCGGCGCTGAAATCGGCTTCCGTGATTTA
GGTAATGCAGATTGGACTAATGGGGCCACCAGTAATGATGCTGGCGTTAAAGGTTCAACGCTAGGCTTAGTAGGTATTTG
GCCTCTAGCTAATCGTTGGAGCTTATCAGCCGAAGCGGGTGCTATGTATTATTCACTTGAGAACAGCCAAAATAATGGCA
TTACATCAAGCAAATACAGTACAAACGACTTTGCTCCATACTTAGGTGCAGGTGTTGGTTACAACTTCACTGATAACCTG
AAGTTACAAGCTAAATATCGTCGTTATGAAAACCTAAATGACGATTACAACATCGTTGAAGCTGATAGCAACTACTGGGG
TCTTGAACTGAGCTACCGTTTCGGTTCACCAGCAGCTGCAGCACCTGTTGCAGCAGCGGTTATCGCAGCAGCACCTGTTG
ACTCAGACAACGATGGTGTTTATGACGACAAAGATCAATGTCCAGCCACACCAGTAACGCATAAAGTTGACTCTGTTGGC
TGTACCATTTACGAAAACGTAAAAAATAAAGAAGACGTGGGTTCAATTCAGTTTGCTAACGATTCTGCTGTAGTTAAAAA
AGTTTTCTACAAAGATATCGAAAGACTAGCTAACTACCTGAACAAGAACCCAGAGTTCACAGTTGAAATCGCTGGCCACG
CCTCTAACGTAGGTAAACCAGAGTACAACATGGTGCTGTCTGACAAACGTGCTGACGCAGTTGCCCAAATTCTTGTTGAG
AAATACGGTATCAGCCAAAGCCGCGTAACTTCTAACGGTTACGGTGTTACTCAGCCATTGGTTGCTGGTGACTCGAAAGA
AGCTTACGCAGCGAACCGTCGTATCGAAGCCATTGTAACGACTACGACTAAACAACCTGTTCTTAAGTAA

Upstream 100 bases:

>100_bases
ATAATTAATCGCTGGCTACAATACCAAAGGATCCCCACATTAAGTGCTAGCAATGTTTTGCACATATACGGCAAGACATA
TTGGTGGAGAATCATAAATA

Downstream 100 bases:

>100_bases
TATTACTTACTGACGTAAAAAAGTCGCTGCTTGCAGCGACTTTTTGTTTTTATGGGGTATACACTAGGACAAGCGATGCC
CTCTACCGCAGCCAAGATGA

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MELENMMNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYMFNDYFGAEIGFRDL
GNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNL
KLQAKYRRYENLNDDYNIVEADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVG
CTIYENVKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAQILVE
KYGISQSRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQPVLK

Sequences:

>Translated_369_residues
MELENMMNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYMFNDYFGAEIGFRDL
GNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNL
KLQAKYRRYENLNDDYNIVEADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVG
CTIYENVKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAQILVE
KYGISQSRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQPVLK
>Mature_369_residues
MELENMMNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWDVFAGYMFNDYFGAEIGFRDL
GNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAGAMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNL
KLQAKYRRYENLNDDYNIVEADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVG
CTIYENVKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAQILVE
KYGISQSRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVTTTTKQPVLK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787191, Length=359, Percent_Identity=23.3983286908078, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 40280; Mature: 40280

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELENMMNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCCEEEE
VFAGYMFNDYFGAEIGFRDLGNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAG
HHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
AMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKYRRYENLNDDYNIVE
EEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEHHHHHCCCCCCCEEEE
ADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVG
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CTIYENVKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKP
EEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
EYNMVLSDKRADAVAQILVEKYGISQSRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVT
CEEEEECCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCEECCCCCHHHHHCCEEEEEEE
TTTKQPVLK
ECCCCCCCC
>Mature Secondary Structure
MELENMMNHKLKFVLLASLLPFTVSAAQELTPWYVGAGLGVNNYEHIATQNGQDDPYAWD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCCEEEE
VFAGYMFNDYFGAEIGFRDLGNADWTNGATSNDAGVKGSTLGLVGIWPLANRWSLSAEAG
HHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
AMYYSLENSQNNGITSSKYSTNDFAPYLGAGVGYNFTDNLKLQAKYRRYENLNDDYNIVE
EEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEHHHHHCCCCCCCEEEE
ADSNYWGLELSYRFGSPAAAAPVAAAVIAAAPVDSDNDGVYDDKDQCPATPVTHKVDSVG
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CTIYENVKNKEDVGSIQFANDSAVVKKVFYKDIERLANYLNKNPEFTVEIAGHASNVGKP
EEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
EYNMVLSDKRADAVAQILVEKYGISQSRVTSNGYGVTQPLVAGDSKEAYAANRRIEAIVT
CEEEEECCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCEECCCCCHHHHHCCEEEEEEE
TTTKQPVLK
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]