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The map label for this gene is ksgA [H]

Identifier: 160874199

GI number: 160874199

Start: 1276613

End: 1277419

Strand: Reverse

Name: ksgA [H]

Synonym: Sbal195_1079

Alternate gene names: 160874199

Gene position: 1277419-1276613 (Counterclockwise)

Preceding gene: 160874200

Following gene: 160874198

Centisome position: 23.89

GC content: 47.83

Gene sequence:

>807_bases
ATGAGCAACAAAGTACATTTAGGCCACACGGCCAGAAAGCGTTTCGGACAAAACTTCCTTACCGACGGCAATGTGATTGA
CCGCATTGTGGGCGCAATTGCCCCCGATAATCACCATGTGATGGTCGAGATCGGCCCAGGCCTTGGTGCCTTAACCGAAC
CCGTAGCCGAAGCTGTAGATAACCTGACCGTCGTCGAACTTGACCGCGATCTAGTTGAACGCTTACATCATCACCCAGTC
CTTAAAGATAAACTGACCATTCACCAAGGTGATGCACTGCAGTTTGATTTTGGTCAACTGAGTGTGCCTGGCAAAAAGAT
GAAAGTGTTTGGTAACTTACCTTACAACATTTCAACGCCGCTGATGTTCCACCTGTTCGAATTTGCCGAACAGATTGAAA
CCATGCACTTTATGCTGCAAAAAGAAGTGGTACTGCGTTTATCGGCAAGCCCAGGTTGTAAAGCCTATGGCCGTTTAACG
GTAATGGCGCAATACTTCTGCCAAGTGGTTCCGGTTCTTGAAGTGCCGCCCCACAGCTTTACGCCCGCGCCTAAGGTCGA
CTCAGCAGTCGTACGCTTATTGCCATACGCTGTAAAACCGTTCCCTTGCAAAGATGTGACCGTACTACGTCACCTTTGTA
CTACGGCGTTCAACATGCGTCGTAAGACTCTGCGTAATAACTTAAAGCACATGTTATCTGACGATGAGTTCGAGCAATTA
GGGATTGACTCAAGTCAACGTCCAGAGCAGATTAGCGTGCAACAGTATGTTGCTATGGCTAACATGGTTTGCGATAAGAA
AGCATAA

Upstream 100 bases:

>100_bases
CGATCTCGCGGGTACAGGCAAAGCCGATATTGGCAGCTTTGTGTGTGCTCTGAATAAAGCCATTGAGTTATCCTCAAAGA
ACTAAACGATTACATTATTA

Downstream 100 bases:

>100_bases
ATATGTCTGGGCACTTAGGTGCCCTTTAACCAGAGCCTAGGAACAAGAATGAGCGCATTGGACACCTCCATTCGGGTTGA
AGTTAAAACTGAATATATAG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSNKVHLGHTARKRFGQNFLTDGNVIDRIVGAIAPDNHHVMVEIGPGLGALTEPVAEAVDNLTVVELDRDLVERLHHHPV
LKDKLTIHQGDALQFDFGQLSVPGKKMKVFGNLPYNISTPLMFHLFEFAEQIETMHFMLQKEVVLRLSASPGCKAYGRLT
VMAQYFCQVVPVLEVPPHSFTPAPKVDSAVVRLLPYAVKPFPCKDVTVLRHLCTTAFNMRRKTLRNNLKHMLSDDEFEQL
GIDSSQRPEQISVQQYVAMANMVCDKKA

Sequences:

>Translated_268_residues
MSNKVHLGHTARKRFGQNFLTDGNVIDRIVGAIAPDNHHVMVEIGPGLGALTEPVAEAVDNLTVVELDRDLVERLHHHPV
LKDKLTIHQGDALQFDFGQLSVPGKKMKVFGNLPYNISTPLMFHLFEFAEQIETMHFMLQKEVVLRLSASPGCKAYGRLT
VMAQYFCQVVPVLEVPPHSFTPAPKVDSAVVRLLPYAVKPFPCKDVTVLRHLCTTAFNMRRKTLRNNLKHMLSDDEFEQL
GIDSSQRPEQISVQQYVAMANMVCDKKA
>Mature_267_residues
SNKVHLGHTARKRFGQNFLTDGNVIDRIVGAIAPDNHHVMVEIGPGLGALTEPVAEAVDNLTVVELDRDLVERLHHHPVL
KDKLTIHQGDALQFDFGQLSVPGKKMKVFGNLPYNISTPLMFHLFEFAEQIETMHFMLQKEVVLRLSASPGCKAYGRLTV
MAQYFCQVVPVLEVPPHSFTPAPKVDSAVVRLLPYAVKPFPCKDVTVLRHLCTTAFNMRRKTLRNNLKHMLSDDEFEQLG
IDSSQRPEQISVQQYVAMANMVCDKKA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=289, Percent_Identity=30.4498269896194, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI7657198, Length=218, Percent_Identity=33.0275229357798, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=62.5468164794007, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25141369, Length=286, Percent_Identity=28.3216783216783, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=32.3943661971831, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=259, Percent_Identity=30.1158301158301, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21357273, Length=285, Percent_Identity=28.4210526315789, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21358017, Length=226, Percent_Identity=32.7433628318584, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30106; Mature: 29975

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKVHLGHTARKRFGQNFLTDGNVIDRIVGAIAPDNHHVMVEIGPGLGALTEPVAEAVD
CCCCEECCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
NLTVVELDRDLVERLHHHPVLKDKLTIHQGDALQFDFGQLSVPGKKMKVFGNLPYNISTP
CCEEEEECHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCCEEEEEECCCCCCCHH
LMFHLFEFAEQIETMHFMLQKEVVLRLSASPGCKAYGRLTVMAQYFCQVVPVLEVPPHSF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCC
TPAPKVDSAVVRLLPYAVKPFPCKDVTVLRHLCTTAFNMRRKTLRNNLKHMLSDDEFEQL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GIDSSQRPEQISVQQYVAMANMVCDKKA
CCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNKVHLGHTARKRFGQNFLTDGNVIDRIVGAIAPDNHHVMVEIGPGLGALTEPVAEAVD
CCCEECCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
NLTVVELDRDLVERLHHHPVLKDKLTIHQGDALQFDFGQLSVPGKKMKVFGNLPYNISTP
CCEEEEECHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCCEEEEEECCCCCCCHH
LMFHLFEFAEQIETMHFMLQKEVVLRLSASPGCKAYGRLTVMAQYFCQVVPVLEVPPHSF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCC
TPAPKVDSAVVRLLPYAVKPFPCKDVTVLRHLCTTAFNMRRKTLRNNLKHMLSDDEFEQL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GIDSSQRPEQISVQQYVAMANMVCDKKA
CCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA