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The map label for this gene is gldA [H]

Identifier: 160874139

GI number: 160874139

Start: 1214987

End: 1216102

Strand: Reverse

Name: gldA [H]

Synonym: Sbal195_1019

Alternate gene names: 160874139

Gene position: 1216102-1214987 (Counterclockwise)

Preceding gene: 160874143

Following gene: 160874138

Centisome position: 22.74

GC content: 50.63

Gene sequence:

>1116_bases
ATGAGCACTGTCGTTCCGCGTACCGTCACTTCCCCTAAAAAATTCATTATCGGCAAGGGCTTATTAGCTCAAATGCATGA
TTATGTTAAGGACTTTGGTGACAATGCTTTTATCATCTGCGATGAATTTATCCTCAGCCGAGTTGAGAATGAAGCGGTCG
CAGGGCTGGATCAAAATGGGATCAGCAACCATCTCGAAAAATTCAACTATGAGTGCAGCGAAGCCGAGATAAAGCGCTTA
GGTGCGCTGGCAGAGCAGCATCATACCAATGTAATTGTTGGAGTTGGCGGGGGTAAAACCTTAGATGCCGCCAAAGCCGT
CGCCTTCTATCAAAAACGGCCAGTGGTGCTCTACCCAACCATTGCTTCAACCGATGCACCATGTACGGCACTTGCCGTGA
TCTATTCCGAAACCGGTGAGTTTGAGCGCTATCTGTTTCTGCCACAAAATCCTGATGCTGTTGTTGCCGACACCGCCATT
ATCGCTGCGGCTCCCGCCCGATTCTTTGCAGCAGGCATAGGCGATGCTCTGGCCACTTACTTTGAAGCCCGCGCCTGTTA
TCAGTCCGATGGCGTCAATCTCGTATTGAAAAAACCTTCCCGCACTGGGCTGGGATTAGCGCAGCTTTGCTATCAATTAC
TGACTGAAAATGTTGAAGCCGCTATGGATGCGGTGAGAAACAAGATAGTCACTCCCGCACTTGAGCAAACCATTGAAGCG
ACGATTTATCTCAGTGGTGTCGGGGCAGAAGCGGGCGGATTAGCCGCAGCTCATGCTGTGAACAACGGTATGTCGGCGGT
AGCTGACCTACATAAAGCCCAACACGGCGAGAAAGTGGTGTTTGGCTTGCTCACCCAGCTAGTACTCGAAAATGCCCCCA
AAGCCGAAATCGATAACGTCATTCACATCATCAAAACCGCGGGACTGCCGCTCACGCTCGCCGATTTGGGGCTAAAAACC
TTTGTCGAAGCAGAGTGGCGCAAAGTGGCTGAGATTGCCTGCGCAGAGGGCGACACTATGGGCAATATGCCGATGCAGTT
AACTGCCGACGATGTCTATCAAGCCATGATTGCCGCCAATGCAATGGCTGAACGCTATAAAAAGCTTGCGGTTTAA

Upstream 100 bases:

>100_bases
CATAATAAAAATATTACCACCACACATGCTGTGAAGATAAATTAAGCGTATGCTAGGGTAAGCATGAAAGGATAACCGTC
ATAAGTGCAAAGGAGGCTCT

Downstream 100 bases:

>100_bases
AGCTCATCAATAACTCCCTTGCCTCACCGCTATGGTCAGCAAGATGGCAAGGGAGATGACAACAGACACGATATTTCCAC
CCTCAATGCCAAGAGTTGGC

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSTVVPRTVTSPKKFIIGKGLLAQMHDYVKDFGDNAFIICDEFILSRVENEAVAGLDQNGISNHLEKFNYECSEAEIKRL
GALAEQHHTNVIVGVGGGKTLDAAKAVAFYQKRPVVLYPTIASTDAPCTALAVIYSETGEFERYLFLPQNPDAVVADTAI
IAAAPARFFAAGIGDALATYFEARACYQSDGVNLVLKKPSRTGLGLAQLCYQLLTENVEAAMDAVRNKIVTPALEQTIEA
TIYLSGVGAEAGGLAAAHAVNNGMSAVADLHKAQHGEKVVFGLLTQLVLENAPKAEIDNVIHIIKTAGLPLTLADLGLKT
FVEAEWRKVAEIACAEGDTMGNMPMQLTADDVYQAMIAANAMAERYKKLAV

Sequences:

>Translated_371_residues
MSTVVPRTVTSPKKFIIGKGLLAQMHDYVKDFGDNAFIICDEFILSRVENEAVAGLDQNGISNHLEKFNYECSEAEIKRL
GALAEQHHTNVIVGVGGGKTLDAAKAVAFYQKRPVVLYPTIASTDAPCTALAVIYSETGEFERYLFLPQNPDAVVADTAI
IAAAPARFFAAGIGDALATYFEARACYQSDGVNLVLKKPSRTGLGLAQLCYQLLTENVEAAMDAVRNKIVTPALEQTIEA
TIYLSGVGAEAGGLAAAHAVNNGMSAVADLHKAQHGEKVVFGLLTQLVLENAPKAEIDNVIHIIKTAGLPLTLADLGLKT
FVEAEWRKVAEIACAEGDTMGNMPMQLTADDVYQAMIAANAMAERYKKLAV
>Mature_370_residues
STVVPRTVTSPKKFIIGKGLLAQMHDYVKDFGDNAFIICDEFILSRVENEAVAGLDQNGISNHLEKFNYECSEAEIKRLG
ALAEQHHTNVIVGVGGGKTLDAAKAVAFYQKRPVVLYPTIASTDAPCTALAVIYSETGEFERYLFLPQNPDAVVADTAII
AAAPARFFAAGIGDALATYFEARACYQSDGVNLVLKKPSRTGLGLAQLCYQLLTENVEAAMDAVRNKIVTPALEQTIEAT
IYLSGVGAEAGGLAAAHAVNNGMSAVADLHKAQHGEKVVFGLLTQLVLENAPKAEIDNVIHIIKTAGLPLTLADLGLKTF
VEAEWRKVAEIACAEGDTMGNMPMQLTADDVYQAMIAANAMAERYKKLAV

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=360, Percent_Identity=50.2777777777778, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1786815, Length=360, Percent_Identity=29.7222222222222, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39676; Mature: 39545

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00913 ADH_IRON_1 ; PS00687 ALDEHYDE_DEHYDR_GLU ; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVVPRTVTSPKKFIIGKGLLAQMHDYVKDFGDNAFIICDEFILSRVENEAVAGLDQNG
CCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHCCCCCCC
ISNHLEKFNYECSEAEIKRLGALAEQHHTNVIVGVGGGKTLDAAKAVAFYQKRPVVLYPT
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEC
IASTDAPCTALAVIYSETGEFERYLFLPQNPDAVVADTAIIAAAPARFFAAGIGDALATY
CCCCCCCHHHHHEEHHCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
FEARACYQSDGVNLVLKKPSRTGLGLAQLCYQLLTENVEAAMDAVRNKIVTPALEQTIEA
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEE
TIYLSGVGAEAGGLAAAHAVNNGMSAVADLHKAQHGEKVVFGLLTQLVLENAPKAEIDNV
EEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHH
IHIIKTAGLPLTLADLGLKTFVEAEWRKVAEIACAEGDTMGNMPMQLTADDVYQAMIAAN
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHH
AMAERYKKLAV
HHHHHHHHHCC
>Mature Secondary Structure 
STVVPRTVTSPKKFIIGKGLLAQMHDYVKDFGDNAFIICDEFILSRVENEAVAGLDQNG
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHCCCCCCC
ISNHLEKFNYECSEAEIKRLGALAEQHHTNVIVGVGGGKTLDAAKAVAFYQKRPVVLYPT
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEC
IASTDAPCTALAVIYSETGEFERYLFLPQNPDAVVADTAIIAAAPARFFAAGIGDALATY
CCCCCCCHHHHHEEHHCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
FEARACYQSDGVNLVLKKPSRTGLGLAQLCYQLLTENVEAAMDAVRNKIVTPALEQTIEA
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEE
TIYLSGVGAEAGGLAAAHAVNNGMSAVADLHKAQHGEKVVFGLLTQLVLENAPKAEIDNV
EEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHH
IHIIKTAGLPLTLADLGLKTFVEAEWRKVAEIACAEGDTMGNMPMQLTADDVYQAMIAAN
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHH
AMAERYKKLAV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]