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The map label for this gene is tuf1 [H]

Identifier: 160873312

GI number: 160873312

Start: 230934

End: 232118

Strand: Direct

Name: tuf1 [H]

Synonym: Sbal195_0186

Alternate gene names: 160873312

Gene position: 230934-232118 (Clockwise)

Preceding gene: 160873310

Following gene: 160873313

Centisome position: 4.32

GC content: 46.67

Gene sequence:

>1185_bases
ATGGCTAAAGCTAAATTTGAACGTATTAAGCCCCATGTAAACGTGGGCACCATTGGTCACGTTGACCATGGTAAAACCAC
ACTGACTGCAGCTATCTCTCACGTACTGGCTAAGACCTACGGTGGCGAAGCTAAAGACTTCTCTCAAATCGATAATGCTC
CAGAAGAGCGTGAGCGCGGTATTACAATCAATACCTCTCACATCGAATATGATACTCCAACTCGTCACTACGCACACGTA
GACTGCCCAGGCCATGCTGACTATGTTAAAAACATGATCACTGGTGCTGCACAGATGGACGGCGCGATTTTAGTAGTAGC
GTCAACAGACGGTCCAATGCCACAGACTCGTGAGCACATCCTGCTTTCTCGTCAGGTTGGCGTACCGTACATCATCGTGT
TCATGAACAAATGTGACATGGTAGATGACGAAGAATTACTGGAATTGGTAGAAATGGAAGTTCGTGAACTTCTGTCAGAA
TACGATTTCCCAGGTGATGACTTACCAGTAATCCAAGGTTCAGCTCTGAAGGCGCTAGAAGGCCAACCAGAGTGGGAAGC
TAAGATTATCGAACTGGCTAACGCACTGGATTCTTACATTCCAGAACCACAACGTGACATCGATAAGCCGTTCCTACTGC
CAATCGAAGACGTATTCTCAATCTCTGGCCGTGGTACTGTAGTAACTGGCCGTGTTGAGCGTGGTATTGTTAAAGTGGGT
GACGAAGTTGAAATCGTTGGTGTTCGCACTACGACTAAAACAACGTGTACTGGCGTAGAAATGTTCCGTAAGCTGCTTGA
CGAAGGTCGTGCAGGCGAGAACTGTGGTGTGTTATTACGTGGTACTAAGCGTGATGACGTAGAACGTGGTCAAGTATTGG
CTAAACCAGGTTCAATCAACCCACACACTACTTTTGAATCAGAAGTTTATGTTCTGTCAAAAGAAGAAGGCGGACGTCAC
ACTCCATTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACCGGTACTATCGAACTGCCAGAAGG
CGTAGAGATGGTAATGCCTGGCGACAACATCAAAATGGTAGTTACTCTGATTTGCCCAATCGCGATGGACGAAGGTTTAC
GCTTCGCAATCCGTGAAGGTGGTCGTACAGTAGGTGCTGGTGTAGTAGCTAAAATCATTGCTTAA

Upstream 100 bases:

>100_bases
TCCTACGCCTAAACATAATCGGTTCGATGTCATTTTAATGCATCGGATTTTTTCTATATGTTAGTTTTCATCACCAAGAT
TCTTGGACTGAGGCATTACC

Downstream 100 bases:

>100_bases
TAGCGATAGAGATTAGTTTGAAAAGGAAGCTTCGGCTTCCTTTTTTGTTTAAGTGGATTATTTCATAGGTTTTGTGGTTT
GCATGGGATTCGCATCCTTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGQPEWEAKIIELANALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKVG
DEVEIVGVRTTTKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLAKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_394_residues
MAKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGQPEWEAKIIELANALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKVG
DEVEIVGVRTTTKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLAKPGSINPHTTFESEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
>Mature_393_residues
AKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERGITINTSHIEYDTPTRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPYIIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGQPEWEAKIIELANALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKVGD
EVEIVGVRTTTKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLAKPGSINPHTTFESEVYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.2151898734177, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=33.0508474576271, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=29.3453724604966, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=29.3453724604966, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=29.3064876957494, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=28.695652173913, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194097354, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI194018520, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI46094014, Length=351, Percent_Identity=29.0598290598291, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4503507, Length=357, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI82546879, Length=430, Percent_Identity=24.8837209302326, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157426893, Length=263, Percent_Identity=25.0950570342205, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=29.8701298701299, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=29.8701298701299, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=87.5634517766497, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=87.5634517766497, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.4429708222812, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789108, Length=311, Percent_Identity=29.5819935691318, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.8796992481203, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=46.6321243523316, Blast_Score=347, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=29.9776286353468, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=29.9776286353468, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=28.175519630485, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.0459770114943, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.0092592592593, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=237, Percent_Identity=32.9113924050633, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=341, Percent_Identity=28.1524926686217, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=152, Percent_Identity=36.1842105263158, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=27.2401433691756, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=28.936170212766, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=64.2317380352645, Blast_Score=522, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.8558558558559, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.8558558558559, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=23.6323851203501, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.6135693215339, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=28.2142857142857, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=282, Percent_Identity=28.7234042553192, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.5063613231552, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.5063613231552, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=383, Percent_Identity=51.9582245430809, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=29.082774049217, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=29.082774049217, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=29.082774049217, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=29.082774049217, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.9532293986637, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.9532293986637, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.4701986754967, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.7522935779816, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=31.2684365781711, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647048, Length=415, Percent_Identity=26.0240963855422, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647046, Length=415, Percent_Identity=26.0240963855422, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28572034, Length=163, Percent_Identity=36.8098159509202, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43350; Mature: 43219

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCCHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GQPEWEAKIIELANALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKVG
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGVRTTTKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLAKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
EEEHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERIKPHVNVGTIGHVDHGKTTLTAAISHVLAKTYGGEAKDFSQIDNAPEERERG
CCCCCHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITINTSHIEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GQPEWEAKIIELANALDSYIPEPQRDIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKVG
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHCCCEEEEC
DEVEIVGVRTTTKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLAKPGSIN
CEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCC
PHTTFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAKIIA
EEEHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA