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The map label for this gene is pelC

Identifier: 16080548

GI number: 16080548

Start: 3590603

End: 3591268

Strand: Direct

Name: pelC

Synonym: BSU34950

Alternate gene names: 16080548

Gene position: 3590603-3591268 (Clockwise)

Preceding gene: 16080547

Following gene: 16080554

Centisome position: 85.17

GC content: 43.54

Gene sequence:

>666_bases
TTGAAAAAAATCGTGTCTATCCTATTTATGTTCGGTTTGGTTATGGGTTTCAGCCAGTTTCAGCCATCAACCGTTTTTGC
AGCTGACAAAGTGGTGCACGAAACAATTATCGTACCAAAAAATACAACATATGACGGGAAAGGACAGCGGTTTGTGGCAG
GGAAAGAATTAGGTGACGGAAGCCAGTCAGAAAACCAAGACCCTGTTTTTCGTGTGGAGGATGGAGCAACCCTGAAAAAT
GTGGTGCTTGGTGCACCTGCAGCTGATGGCGTGCACACTTATGGAAACGTTAACATTCAGAATGTGAAATGGGAAGATGT
TGGTGAGGATGCGTTAACGGTGAAGAAGGAAGGAAAAGTGACGATCGACGGCGGTTCTGCTCAAAAAGCGTCAGATAAGA
TATTCCAAATCAATAAAGCCAGTACCTTCACAGTGAAAAATTTCACGGCGGATAATGGCGGGAAGTTCATTAGACAGCTT
GGTGGTTCAACCTTCCACGTTGATGTGATCATCGACAAGTGCACCATCACAAACATGAAGGAAGCGATATTCCGGACGGA
CAGCAAAACAAGCACGGTCAGAATGACAAATACACGCTACTCTAATGTCGGTCAAAAATGGATTGGTGTTCAGCATATTT
ACGAAAACAACAACACTCAATTTTAA

Upstream 100 bases:

>100_bases
AAGCAGATTCAGCCTTGCCCCGATCTATGCGAGAGAAGCCGGTGCAAGTCGCTGACGCTTCTCTTGCAAAAAATGAAAGA
TATCCGGGGAGGATTTATCA

Downstream 100 bases:

>100_bases
CAAAAAAAGTCCGCTGATGTTATTCAGCGGACTTTTTCAATCTTTCTTGCATTTCTTCCTTCGTATAGATGATCCTCATC
GGATTTCCGCCGACAAATGC

Product: secreted pectate lyase

Products: NA

Alternate protein names: Pectin lyase

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKKIVSILFMFGLVMGFSQFQPSTVFAADKVVHETIIVPKNTTYDGKGQRFVAGKELGDGSQSENQDPVFRVEDGATLKN
VVLGAPAADGVHTYGNVNIQNVKWEDVGEDALTVKKEGKVTIDGGSAQKASDKIFQINKASTFTVKNFTADNGGKFIRQL
GGSTFHVDVIIDKCTITNMKEAIFRTDSKTSTVRMTNTRYSNVGQKWIGVQHIYENNNTQF

Sequences:

>Translated_221_residues
MKKIVSILFMFGLVMGFSQFQPSTVFAADKVVHETIIVPKNTTYDGKGQRFVAGKELGDGSQSENQDPVFRVEDGATLKN
VVLGAPAADGVHTYGNVNIQNVKWEDVGEDALTVKKEGKVTIDGGSAQKASDKIFQINKASTFTVKNFTADNGGKFIRQL
GGSTFHVDVIIDKCTITNMKEAIFRTDSKTSTVRMTNTRYSNVGQKWIGVQHIYENNNTQF
>Mature_221_residues
MKKIVSILFMFGLVMGFSQFQPSTVFAADKVVHETIIVPKNTTYDGKGQRFVAGKELGDGSQSENQDPVFRVEDGATLKN
VVLGAPAADGVHTYGNVNIQNVKWEDVGEDALTVKKEGKVTIDGGSAQKASDKIFQINKASTFTVKNFTADNGGKFIRQL
GGSTFHVDVIIDKCTITNMKEAIFRTDSKTSTVRMTNTRYSNVGQKWIGVQHIYENNNTQF

Specific function: Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins. Is also a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 3 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLYC_BACSU (O34310)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   A70045
- RefSeq:   NP_391375.1
- ProteinModelPortal:   O34310
- SMR:   O34310
- EnsemblBacteria:   EBBACT00000002312
- GeneID:   936594
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34950
- NMPDR:   fig|224308.1.peg.3501
- GenoList:   BSU34950
- GeneTree:   EBGT00050000006466
- HOGENOM:   HBG349026
- OMA:   EAIFRTD
- ProtClustDB:   CLSK238008
- BioCyc:   BSUB:BSU34950-MONOMER
- BRENDA:   4.2.2.2
- InterPro:   IPR004898
- InterPro:   IPR012334
- InterPro:   IPR011050
- Gene3D:   G3DSA:2.160.20.10

Pfam domain/function: PF03211 Pectate_lyase; SSF51126 Pectin_lyas_like

EC number: =4.2.2.2; =4.2.2.10

Molecular weight: Translated: 24282; Mature: 24282

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVSILFMFGLVMGFSQFQPSTVFAADKVVHETIIVPKNTTYDGKGQRFVAGKELGDG
CHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCCC
SQSENQDPVFRVEDGATLKNVVLGAPAADGVHTYGNVNIQNVKWEDVGEDALTVKKEGKV
CCCCCCCCEEEEECCCEEEHEEEECCCCCCCEECCCEEEEEEECCCCCCCEEEEEECCEE
TIDGGSAQKASDKIFQINKASTFTVKNFTADNGGKFIRQLGGSTFHVDVIIDKCTITNMK
EEECCCCCCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHCCCEEEEEEEEEEEECCCHH
EAIFRTDSKTSTVRMTNTRYSNVGQKWIGVQHIYENNNTQF
HHHEECCCCCCEEEEECCCCCCCCHHHHCEEEEEECCCCCC
>Mature Secondary Structure
MKKIVSILFMFGLVMGFSQFQPSTVFAADKVVHETIIVPKNTTYDGKGQRFVAGKELGDG
CHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCCC
SQSENQDPVFRVEDGATLKNVVLGAPAADGVHTYGNVNIQNVKWEDVGEDALTVKKEGKV
CCCCCCCCEEEEECCCEEEHEEEECCCCCCCEECCCEEEEEEECCCCCCCEEEEEECCEE
TIDGGSAQKASDKIFQINKASTFTVKNFTADNGGKFIRQLGGSTFHVDVIIDKCTITNMK
EEECCCCCCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHCCCEEEEEEEEEEEECCCHH
EAIFRTDSKTSTVRMTNTRYSNVGQKWIGVQHIYENNNTQF
HHHEECCCCCCEEEEECCCCCCCCHHHHCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377