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The map label for this gene is ytcB

Identifier: 16080139

GI number: 16080139

Start: 3157008

End: 3157958

Strand: Direct

Name: ytcB

Synonym: BSU30870

Alternate gene names: 16080139

Gene position: 3157008-3157958 (Clockwise)

Preceding gene: 16080138

Following gene: 16080140

Centisome position: 74.89

GC content: 46.79

Gene sequence:

>951_bases
ATGAAAATACTCGTCACAGGAGCAGCGGGCTTTATCGGCTCCCACCTCTGCGAAGAATTACTAAAAGATAAGAAACATAA
CGTTATCGGAATCGATGACTTTATCGGTCCTACTCCATTTTCCTTGAAATTAAAAAACCTTAAAAACCTGCTGCCGGAAA
AGCGATTTACGTTCATAAAGGAAAATCTGCTGACAGCCGATCTTGCTTCCTTACTGGAAGGAGTGGACGTCATCTTTCAT
TTGGCGGCCATACCGGGTGTCCGCTCAAGCTGGGGCAATCATTTTCACCCATATGCCGCACATAATATCCAAGCGCTCCA
AAGGCTCCTCGAGGCGTGCCGGGAACATTCTATTCAAACGTTCGTCTTTGCATCCACCTCTTCCGTCTATGGCGAAAAAC
AAGGAAAAGTCAGCGAAAACACGTCGCTTTCTCCTTTATCTCCGTACGGGGTAACAAAGCTTACGGGAGAAAAGCTTTGC
CATGTGTATAAACAGAGCTTTGGCATTCCAATTGTGATTCTCCGTTTTTTTACCGTATACGGGCCAAGACAGCGGCCGGA
TATGGCTTTTCACCGGCTCATTAAGCAGCACCTTCAGCAAAAGCCGCTTACCATCTTCGGTGATGGGCAGCAGTCAAGAG
ATTTCACTTATATCAGCGACTGTGTCAAAGGCATCACCGCTGTTCTTGGGAAACCCCACCTTATTGGTGAAACAGTAAAT
ATCGGCGGCGCAGAGCGTGCCTCAGTCTTAAAAGTTGTCTCCCTCATTGAGGATATTTCCGGGAGAAAAGCAACACTGCA
CTTTTCGGACAAAATAGCAGGGGAACCGAGCAACACGTGGGCGGATATTTCAAAAGCAAAACAGCTCTTGCATTATGATC
CCGCCACATCCTTAAAGGATGGCTTGACCAACGAAATCGCCTATTTATCGTCGCTGTATCAGGGGGAATAA

Upstream 100 bases:

>100_bases
CAGATATCATGAAAGGGCGTCTTGTGATTGACGGACGCAACGTACTAAAAAAAGAACTGATCGAGGCTTGCGGCCTGATC
TGTACGGGAGTTGGCCGTCC

Downstream 100 bases:

>100_bases
AAATGAAGCTTGCCTTTATCTGTACAGAAAAGCTGCCGGCGCCCGCAGTACGCGGCGGCGCTATTCAAATGATGATTGAC
GGTGTCACGCCCTATTTCAG

Product: UDP-glucose epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIKENLLTADLASLLEGVDVIFH
LAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQGKVSENTSLSPLSPYGVTKLTGEKLC
HVYKQSFGIPIVILRFFTVYGPRQRPDMAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVN
IGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE

Sequences:

>Translated_316_residues
MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIKENLLTADLASLLEGVDVIFH
LAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQGKVSENTSLSPLSPYGVTKLTGEKLC
HVYKQSFGIPIVILRFFTVYGPRQRPDMAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVN
IGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE
>Mature_316_residues
MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIKENLLTADLASLLEGVDVIFH
LAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQGKVSENTSLSPLSPYGVTKLTGEKLC
HVYKQSFGIPIVILRFFTVYGPRQRPDMAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVN
IGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family

Homologues:

Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=30.0632911392405, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI7657641, Length=320, Percent_Identity=28.4375, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=29.1666666666667, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786974, Length=343, Percent_Identity=27.9883381924198, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=27.6657060518732, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790049, Length=325, Percent_Identity=25.2307692307692, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=27.8996865203762, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=28.3132530120482, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=324, Percent_Identity=27.4691358024691, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=335, Percent_Identity=28.955223880597, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=334, Percent_Identity=29.0419161676647, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=27.6729559748428, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19923002, Length=320, Percent_Identity=28.125, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YTCB_BACSU (O34886)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   H69988
- RefSeq:   NP_390965.1
- HSSP:   O60547
- ProteinModelPortal:   O34886
- SMR:   O34886
- EnsemblBacteria:   EBBACT00000003343
- GeneID:   938900
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30870
- NMPDR:   fig|224308.1.peg.3090
- GenoList:   BSU30870
- GeneTree:   EBGT00070000031836
- HOGENOM:   HBG755066
- OMA:   WADISKA
- PhylomeDB:   O34886
- ProtClustDB:   CLSK873281
- BioCyc:   BSUB:BSU30870-MONOMER
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01370 Epimerase

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 34913; Mature: 34913

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: ACT_SITE 149-149 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIK
CEEEEECCHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCHHEEHHHHHHHCCHHHHHHHH
ENLLTADLASLLEGVDVIFHLAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQT
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
FVFASTSSVYGEKQGKVSENTSLSPLSPYGVTKLTGEKLCHVYKQSFGIPIVILRFFTVY
EEEECCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GPRQRPDMAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVN
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
IGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKD
CCCHHHHHHHHHHHHHHHCCCCEEEEEECHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
GLTNEIAYLSSLYQGE
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIK
CEEEEECCHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCHHEEHHHHHHHCCHHHHHHHH
ENLLTADLASLLEGVDVIFHLAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQT
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
FVFASTSSVYGEKQGKVSENTSLSPLSPYGVTKLTGEKLCHVYKQSFGIPIVILRFFTVY
EEEECCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GPRQRPDMAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVN
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
IGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKD
CCCHHHHHHHHHHHHHHHCCCCEEEEEECHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
GLTNEIAYLSSLYQGE
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377