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The map label for this gene is melA

Identifier: 16080082

GI number: 16080082

Start: 3100881

End: 3102179

Strand: Direct

Name: melA

Synonym: BSU30300

Alternate gene names: 16080082

Gene position: 3100881-3102179 (Clockwise)

Preceding gene: 16080081

Following gene: 16080086

Centisome position: 73.56

GC content: 44.96

Gene sequence:

>1299_bases
ATGAAAAAGATTACATTTATCGGTGCAGGGAGCACGATTTTCGCCAAAAATGTTTTGGGAGACTGCCTGTTAACAGAGGC
ACTGAATGGATTTGAGTTCGCTCTTTACGACATTGATCCCAAACGCCTGCAGGAATCCCAGCTCATGCTCGAAAATCTAA
GAGACCGTTATAACCCAAGTGTGGCCATCAACAGCTATGATGACAGAAAACTCGCCTTGCAAAACGCAGGCTATGTCATC
AATGCGATTCAGGTTGGAGGATACAAACCGAGCACAGTCATCGATTTCGAGATTCCTAAACGGTATGGGCTGCGCCAGAC
GATCGCCGATACAGTAGGCATCGGCGGGATATTCAGATCTCTCAGAACAATCCCGGTCTTATTTGATATCGCAAAAGATA
TGGAGGAAATGTGCCCTGACGCATGGTTCTTAAACTATACAAATCCAATGGCCACTCTTACAGGCGCCATGCTCCGCTAT
ACCAATATCAAAACAATCGGGCTCTGCCACAGTGTTCAAGTGTGCACGAAGGATTTATTTAAAGCTCTCGGAATGGAGCA
TGACGGAATTGAGGAACGCATTGCGGGCATTAATCATATGGCTTGGCTTTTGGAAGTCAAAAAAGACGGCACAGATTTAT
ATCCAGAAATCAAAAGGAGAGCGAAAGAAAAACAAAAAACAAAGCATCATGATATGGTGCGGTTTGAATTAATGGATAAG
TTCGGCTATTATGTCACGGAATCGTCCGAACATAACGCAGAGTATCACCCGTACTTTATTAAACGGAATTACCCTGAACT
GATCAGCGAGCTGCAAATCCCGCTCGACGAATATCCGAGAAGGTGCGTCAAGCAGATTGAAAATTGGGAGAAAATGCGGG
ATGATATCGTCAATAATAAAAACCTTACGCACGAACGCTCAAAGGAATATGGTTCAAGAATTATAGAAGCAATGGAAACA
AACGAACCATTCACCTTCGGAGGAAACGTCTTGAATACAGGGCTGATCACCAACCTTCCTTCAAAAGCGGTTGTGGAAGT
GACATGCGTCGCCGACAGAAAAAAAATTACACCGTGCTTCGCCGGAGAACTGCCTGAGCAGCTTGCAGCCTTAAACCGGA
CGAATATTAATACACAGCTGATGACAATCGAAGCGGCTGTCACACGAAAAAAAGAAGCGGTTTACCAAGCTGCAATGCTT
GATCCGCATACAAGCGCGGAGCTTTCCATGAAGGACATCATCTCCATGTGCGACGATTTATTTGCAGCACACGGCGATTG
GCTTCCGGAATACAAATAA

Upstream 100 bases:

>100_bases
ATGCTGCCGATGGTTGTTCTGTTTTTGGCGCTGCAAAAATACTTTATTGCCGGCCTGTCTTCCGGAGCGGTAAAGGGTTA
ACATTAAAGGGGGAAGCAAT

Downstream 100 bases:

>100_bases
AAACTAGGGGACCGCTCTCCCCTAGTTTTTTGGTTTTGTTTCGCCTTCCCAGGCCATCATGCCGCCTTCAACATTGACTG
TTTTAAAGCCTTGCTCATCC

Product: alpha-D-galactoside galactohydrolase

Products: NA

Alternate protein names: Melibiase

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPSVAINSYDDRKLALQNAGYVI
NAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRSLRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRY
TNIKTIGLCHSVQVCTKDLFKALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDK
FGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNKNLTHERSKEYGSRIIEAMET
NEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCFAGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAML
DPHTSAELSMKDIISMCDDLFAAHGDWLPEYK

Sequences:

>Translated_432_residues
MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPSVAINSYDDRKLALQNAGYVI
NAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRSLRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRY
TNIKTIGLCHSVQVCTKDLFKALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDK
FGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNKNLTHERSKEYGSRIIEAMET
NEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCFAGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAML
DPHTSAELSMKDIISMCDDLFAAHGDWLPEYK
>Mature_432_residues
MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPSVAINSYDDRKLALQNAGYVI
NAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRSLRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRY
TNIKTIGLCHSVQVCTKDLFKALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDK
FGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNKNLTHERSKEYGSRIIEAMET
NEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCFAGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAML
DPHTSAELSMKDIISMCDDLFAAHGDWLPEYK

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family

Homologues:

Organism=Escherichia coli, GI1790560, Length=443, Percent_Identity=49.4356659142212, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1788029, Length=448, Percent_Identity=26.5625, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AGAL_BACSU (O34645)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   E69656
- RefSeq:   NP_390908.1
- ProteinModelPortal:   O34645
- SMR:   O34645
- EnsemblBacteria:   EBBACT00000003353
- GeneID:   937255
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30300
- NMPDR:   fig|224308.1.peg.3033
- GenoList:   BSU30300
- GeneTree:   EBGT00050000000927
- HOGENOM:   HBG511715
- OMA:   MAINTWA
- PhylomeDB:   O34645
- ProtClustDB:   PRK15076
- BioCyc:   BSUB:BSU30300-MONOMER
- BRENDA:   3.2.1.22
- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00732

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =3.2.1.22

Molecular weight: Translated: 49264; Mature: 49264

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: ACT_SITE 170-170 BINDING 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPS
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
VAINSYDDRKLALQNAGYVINAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRS
EEEECCCCCCHHECCCCEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRYTNIKTIGLCHSVQVCTKDLF
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
KALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDK
HHHCCCCCCHHHHHHCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNK
HCCEEECCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
NLTHERSKEYGSRIIEAMETNEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCF
CCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCHHCCCCCCCEEEEEEECCCCCCCCHH
AGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAMLDPHTSAELSMKDIISMCDDL
HCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FAAHGDWLPEYK
HHHCCCCCCCCC
>Mature Secondary Structure
MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPS
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
VAINSYDDRKLALQNAGYVINAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRS
EEEECCCCCCHHECCCCEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRYTNIKTIGLCHSVQVCTKDLF
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
KALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDK
HHHCCCCCCHHHHHHCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNK
HCCEEECCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
NLTHERSKEYGSRIIEAMETNEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCF
CCCHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCHHCCCCCCCEEEEEEECCCCCCCCHH
AGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAMLDPHTSAELSMKDIISMCDDL
HCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FAAHGDWLPEYK
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377