Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is dinF

Identifier: 16079230

GI number: 16079230

Start: 2288669

End: 2290006

Strand: Reverse

Name: dinF

Synonym: BSU21710

Alternate gene names: 16079230

Gene position: 2290006-2288669 (Counterclockwise)

Preceding gene: 16079231

Following gene: 16079228

Centisome position: 54.32

GC content: 44.69

Gene sequence:

>1338_bases
ATGAAAGCATACGATTTTACACAGGGGAACGTGCTGAAACAGCTTGTACTGTTTTCAATGCCTATTATGCTCGGAAACGC
ATTGCAGGTTTCCTTTCAATTTATAGACAGCTTGTGGATCGGCAATTTACTGGGGGCAAAGGCCCTTGGGGCAGCCGCGG
TATCAAGCACGATTGTTTTGACCGTGCTGTCTTTTATTTTGGGCCTTAACAATGCGACGTTAACCATTTTATCTCAGCAA
AAAGGAAAAGATGATATAAAAGGAATGGCGTCGTATATTAACGCTTTTGTTGTTTTATTAACAGGCCTAAGCATCGGATT
CGGCGCGGCCGGTTTTTTTCTCTCAGAGCTTTTGCTGCGTCTTCTAAAAACGCCGGAGTCAATGATACCGCTTGCTGAGA
CGTATTTACAGATTCAATTTATCGGCATTTTATTTTTGTTTGGTTATAACTTTATCAGTACTGTGCTTCGTGCGCTGGGC
GACAGCAAGACACCTCTCCGGTTTATTGCGTTTGCTGTTGTACTGAATACAGTGCTTGCGCCGCTCTTTATTTCTGTGTT
CCGCATGGGAATAGCGGGAGCGGCTTATTCAACGATTTTGTCACAGGGCATTGCGTTTTTGTATGGGTTGTTTTATGTCA
TCAAACACAAGCTTGTGCCATTCTCGATTCCGAGAATGCCCAAGTGGGAAGAGTCCGCGCTGATTTTGAAACTCGGGATT
CCGGCAGGTCTGCAGATGATGGTGATTACAGGCGGTATGATGGCGATTATGAGTGTTGTTAACTCATACGGGGATCACGT
GGTATCAGGCTTTGGCGCGGTGCAGCGGCTTGATAGCATCATCACGCTTCCAGCAATGGCTGCCGGTACGGCCGTCAATA
GTATGGCAGGGCAAAATATTGGAATCGGAAACGAAAAAAGAGTGGGAACAATTGCAAGGCTAGGTGTGATAGCTGTTATT
TCCTGCATGCTGGTGATTGCGGTTATGATTTGGGTATTTGGTAAATACCTCATAAGGTTATTTATCAGTGAGCCTGATGC
GGTCGCATTTGGTGAACAATATTTGAAGTGGATCGCGTTTTTCTACCCTTTTATTGGGGTTAATTTTGTGCTGAACGGAA
TCGTCAGGGCCGCGGGAGCGATGCTGCAGGTTCTGGTATTAAATTTGATCTCGTTTTGGGTGTTGAGGTATCCTTTCACA
GCTCTATTTTCAGCTTGGCTTGGACAAAAAGGAATCGGGCTTGGAATCGGAATGAGTTTTTTATTTAGCAGCTGCGCGGC
ATTTTTGTATTATAGATATGGCCGCTGGAAAGCAATGAAGCTGTTTACTGAAAAATAA

Upstream 100 bases:

>100_bases
GAGACCGAAAAAAAACCGATAAGTCATGAAAAAAGAGACATGAAACAACGTCTCTTTTTTCGTGTGGTCCGAAGAAAATT
TGAGAACACAAGGAGAAAAG

Downstream 100 bases:

>100_bases
GTGAAGCACTGCGAAAAGCACATACCTATATATCGGTATGTGCTTTTTTTCACCGTTTTTTATCAAGTTTGCTGAAGAGC
TCGATTAACGTGGCAAGTTC

Product: damage inducible, Na+ driven multidrug efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKAYDFTQGNVLKQLVLFSMPIMLGNALQVSFQFIDSLWIGNLLGAKALGAAAVSSTIVLTVLSFILGLNNATLTILSQQ
KGKDDIKGMASYINAFVVLLTGLSIGFGAAGFFLSELLLRLLKTPESMIPLAETYLQIQFIGILFLFGYNFISTVLRALG
DSKTPLRFIAFAVVLNTVLAPLFISVFRMGIAGAAYSTILSQGIAFLYGLFYVIKHKLVPFSIPRMPKWEESALILKLGI
PAGLQMMVITGGMMAIMSVVNSYGDHVVSGFGAVQRLDSIITLPAMAAGTAVNSMAGQNIGIGNEKRVGTIARLGVIAVI
SCMLVIAVMIWVFGKYLIRLFISEPDAVAFGEQYLKWIAFFYPFIGVNFVLNGIVRAAGAMLQVLVLNLISFWVLRYPFT
ALFSAWLGQKGIGLGIGMSFLFSSCAAFLYYRYGRWKAMKLFTEK

Sequences:

>Translated_445_residues
MKAYDFTQGNVLKQLVLFSMPIMLGNALQVSFQFIDSLWIGNLLGAKALGAAAVSSTIVLTVLSFILGLNNATLTILSQQ
KGKDDIKGMASYINAFVVLLTGLSIGFGAAGFFLSELLLRLLKTPESMIPLAETYLQIQFIGILFLFGYNFISTVLRALG
DSKTPLRFIAFAVVLNTVLAPLFISVFRMGIAGAAYSTILSQGIAFLYGLFYVIKHKLVPFSIPRMPKWEESALILKLGI
PAGLQMMVITGGMMAIMSVVNSYGDHVVSGFGAVQRLDSIITLPAMAAGTAVNSMAGQNIGIGNEKRVGTIARLGVIAVI
SCMLVIAVMIWVFGKYLIRLFISEPDAVAFGEQYLKWIAFFYPFIGVNFVLNGIVRAAGAMLQVLVLNLISFWVLRYPFT
ALFSAWLGQKGIGLGIGMSFLFSSCAAFLYYRYGRWKAMKLFTEK
>Mature_445_residues
MKAYDFTQGNVLKQLVLFSMPIMLGNALQVSFQFIDSLWIGNLLGAKALGAAAVSSTIVLTVLSFILGLNNATLTILSQQ
KGKDDIKGMASYINAFVVLLTGLSIGFGAAGFFLSELLLRLLKTPESMIPLAETYLQIQFIGILFLFGYNFISTVLRALG
DSKTPLRFIAFAVVLNTVLAPLFISVFRMGIAGAAYSTILSQGIAFLYGLFYVIKHKLVPFSIPRMPKWEESALILKLGI
PAGLQMMVITGGMMAIMSVVNSYGDHVVSGFGAVQRLDSIITLPAMAAGTAVNSMAGQNIGIGNEKRVGTIARLGVIAVI
SCMLVIAVMIWVFGKYLIRLFISEPDAVAFGEQYLKWIAFFYPFIGVNFVLNGIVRAAGAMLQVLVLNLISFWVLRYPFT
ALFSAWLGQKGIGLGIGMSFLFSSCAAFLYYRYGRWKAMKLFTEK

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI153792564, Length=385, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YPNP_BACSU (P54181)

Other databases:

- EMBL:   L77246
- EMBL:   AL009126
- PIR:   D69939
- RefSeq:   NP_390054.1
- ProteinModelPortal:   P54181
- EnsemblBacteria:   EBBACT00000000132
- GeneID:   939104
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU21710
- NMPDR:   fig|224308.1.peg.2177
- GenoList:   BSU21710
- GeneTree:   EBGT00050000000379
- HOGENOM:   HBG458967
- OMA:   WILASTI
- ProtClustDB:   CLSK238317
- BioCyc:   BSUB:BSU21710-MONOMER
- InterPro:   IPR002528
- InterPro:   IPR015522
- PANTHER:   PTHR11206
- PANTHER:   PTHR11206:SF6
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 48477; Mature: 48477

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x108e5f50)-; HASH(0xb35db78)-; HASH(0xdc3a36c)-; HASH(0x105b32ac)-; HASH(0x1053b708)-; HASH(0x1045a788)-; HASH(0xfc22554)-; HASH(0x103a7e98)-; HASH(0xff8448c)-; HASH(0xfab8a54)-; HASH(0xf1085cc)-; HASH(0xfd00710)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure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HHHHHHHHHHHHHCCHHHHHHHCCH
>Mature Secondary Structure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HHHHHHHHHHHHHCCHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377