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The map label for this gene is yokF

Identifier: 16079220

GI number: 16079220

Start: 2279977

End: 2280867

Strand: Direct

Name: yokF

Synonym: BSU21610

Alternate gene names: 16079220

Gene position: 2279977-2280867 (Clockwise)

Preceding gene: 16079211

Following gene: 16079221

Centisome position: 54.08

GC content: 38.95

Gene sequence:

>891_bases
TTGAAGAAAGTATTATTAGGTTTTGCAGCATTCACTTTGAGCTTATCGTTGGCAGCCTGCAGCTCAAATGATTCTGAAAA
AGTAAGCACAGAAAAAGAAACACCACAAGCGTCTACGGATGTGGAAAAGAAAACAGAACAAAAGGAATCTACTAAAGAAA
AAACTGCTGACAAGTCTAAGGAAAAAGACAAAAAGGAATTGGTCGATGTTACTCTAGATAGAGCTGTTGATGGTGACACA
ATTAAAGTTACCTACAACGGAAATGTAGACACAGTACGCTACTTGCTCATAGACACACCTGAAACAAAGAAACCAAATTC
TTGTGTTCAACCATATGGTGAGGATGCGTCAAAACGAAATAAAGAATTGGTCAACAGTGGTAAGCTGCAGCTTGAATTTG
ACAAAGGTGATCGCAGAGATAAATACGGAAGACTATTAGCTTATGTTTATGTGGATGGCAAATCTGTTCAGGAAACATTG
CTAAAAGAAGGATTGGCCAGAGTAGCGTATGTATATGAGCCAAATACGAAATACATAGATCAATTTAAGAAAGACGAGCA
AGAAGCAAAATCAGAGAAGCTTTCAATCTGGAGCAAGAATGGATATGTGACTGACCGAGGATTTAATGGCTGTGTGAAAG
AGAAAACCACTGCAGTTAAAAAAGCAACAACATCTAAACCGGCAGCAACACAGCCTACAACTCCAAAGGCATCAAGTGAA
ACTTCGACTACAACTGAAAAAGAAGCTTCTTCAGAGACAACTGGAGGAACAGAAACATTTAAAAATTGCACAGAGTTAAG
AAAGAAATATCCGAATGGAGTGCCTAGCTCCCACCCTGCTTACCAATCTAAAATGGACAGGGATCATGACAACTATGCTT
GTGAACGATGA

Upstream 100 bases:

>100_bases
TAAGGAATAATTTAATTCACAAAAATTGGATACAGGATTATAATTATTGATGGTTTACCGATATAAATAGAGAACAAACA
TTCTAAAAGGGGAAATGAAG

Downstream 100 bases:

>100_bases
AAGGAGATAAGTGATGGGACAAAAATTTCATAAATTAAACTCTAAGTATGGAATCATAGATTATCCAATACTTTTAAAAG
ATCTTGAGTCCATCATTCAA

Product: SPbeta phage DNA nuclease, lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDT
IKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRNKELVNSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETL
LKEGLARVAYVYEPNTKYIDQFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSE
TSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER

Sequences:

>Translated_296_residues
MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDT
IKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRNKELVNSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETL
LKEGLARVAYVYEPNTKYIDQFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSE
TSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER
>Mature_296_residues
MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDT
IKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRNKELVNSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETL
LKEGLARVAYVYEPNTKYIDQFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSE
TSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER

Specific function: Catalyzes the hydrolysis of supercoiled double and single strand DNA and RNA. Involved in chromosomal DNA degradation and cell death caused by thermal stress

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YOKF_BACSU (O32001)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_390044.1
- HSSP:   P00644
- ProteinModelPortal:   O32001
- SMR:   O32001
- EnsemblBacteria:   EBBACT00000000668
- GeneID:   939113
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU21610
- NMPDR:   fig|224308.1.peg.2167
- GenoList:   BSU21610
- GeneTree:   EBGT00050000000321
- HOGENOM:   HBG752546
- OMA:   IDTPETK
- ProtClustDB:   CLSK409325
- BioCyc:   BSUB:BSU21610-MONOMER
- InterPro:   IPR008613
- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071
- Gene3D:   G3DSA:2.40.50.90
- SMART:   SM00894
- SMART:   SM00318

Pfam domain/function: PF05901 Excalibur; PF00565 SNase; SSF50199 Staphylococal_nuclease_OB-fold

EC number: NA

Molecular weight: Translated: 33003; Mature: 33003

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS01123 TNASE_1; PS01284 TNASE_2; PS50830 TNASE_3; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 93-93 ACT_SITE 101-101 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSK
CCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKDKKELVDVTLDRAVDGDTIKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRN
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHH
KELVNSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETLLKEGLARVAYVYEPNTKYID
HHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHEEEEECCCHHHHH
QFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSE
HHHHHHHHHHHHHEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSK
CCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKDKKELVDVTLDRAVDGDTIKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRN
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHH
KELVNSGKLQLEFDKGDRRDKYGRLLAYVYVDGKSVQETLLKEGLARVAYVYEPNTKYID
HHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHEEEEECCCHHHHH
QFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSE
HHHHHHHHHHHHHEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377