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The map label for this gene is blyA

Identifier: 16079200

GI number: 16079200

Start: 2263489

End: 2264592

Strand: Direct

Name: blyA

Synonym: BSU21410

Alternate gene names: 16079200

Gene position: 2263489-2264592 (Clockwise)

Preceding gene: 16079199

Following gene: 16079201

Centisome position: 53.69

GC content: 37.59

Gene sequence:

>1104_bases
ATGTCAGTTTTCACTAATAGCTACATTCCAGTCAATAAGTATACTAGACCAGGTTTGAAATTACAGGGTGTGAAAAAATG
CGTCCTACACTATACAGCCAATCCGGGTGCAGGTGCAGACAATCATCGAAGATACTTCAGTAATGCACAAGTTTATGCAT
CAGCTCACATTTTCGTAGATAAGGCTGAAGCAATTTGTATCATTCCATTAAATGAAGTAGCTTACCATGCAAATGATATT
CAGCAAAGAGATAGTGCCGGAAATCCTTATCGAGGAGTTGCTGCGCTGAAACCTAACGCTAACTTTCTTTCTATTGGAGT
TGAAATGTGCCTTGAAAAAGACGGTTCATTCCATTCAGATACAGTTGAAAGAACTGAGGATGTGTTCGTTGAATTATGTA
ATAAGTTTGGTTTAGATCCTATTGATGATATTGTTCGTCATTATGACATCACCCATAAGAATTGCCCTGCACCATGGGTA
TCTAACAGCCAAAAATTTGTAGACTTTAAAAATCGAGTAAAGGCAAAAATGTCAGGCAAATCTGTTTCAAAAGCTTCTCC
AACTAAACCAACAACCTCCTCCCCTTCCTCTTCATCAGCAGTAAGTGGTTCACTAAAATCAAAAGTTGACGGACTTCGCT
TCTATTCAAAACCATCTTGGGAAGATAAAGATGTTGTCGGCACAGTAAATAAAGGCATCGGATTTCCTACAGTTGTAGAG
AAAGTTAAAGTTGGATCTGCCTATCAATACAAAGTTAAGAACTCAAAAGGCACTACATATTACATCACTGCTTCTGACAA
ATATGTTGATGTTACAGGATCAGTTAAAACCTCTTCCTCTGCCCCAAAAACAACATCAACTTCTTCAAGTTCCTCATCTA
TTAAATCCGTAGGAAAAATCAAAATTGTCGGTGTATCAAGCGCTGCAATCGTAATGGACAAGCCTGATCGAAATAGTTCT
AAAAATATTGGCACAGTTAAGCTTGGAAGCACTATTTCAATTTCTGGTTCAGTTAAAGGTAAAAACAATTCCAATGGCTA
CTGGGAAGTTATTTATAAAGGTAAACGTGGATATATCTCGGGACAGTTTGGATCAACAATCTAA

Upstream 100 bases:

>100_bases
ACATCCAACAATAATGAGATTCAATTCAACGAGTTTCGAAATCGCTATGAAAATGGTGAATTCGAGGCTCTTTTTAATTA
GAAATTAAGGAGATGTTTTT

Downstream 100 bases:

>100_bases
TTATATTCAATTTTCTCGGAGGATGTTTATTGATCAATATGTTTCAGTAAATATCCTTTTCTATGGTACTAAGGTGGTGA
ACATTTTATGGAAATGGATA

Product: bacteriophage SPbeta N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSVFTNSYIPVNKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRYFSNAQVYASAHIFVDKAEAICIIPLNEVAYHANDI
QQRDSAGNPYRGVAALKPNANFLSIGVEMCLEKDGSFHSDTVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWV
SNSQKFVDFKNRVKAKMSGKSVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWEDKDVVGTVNKGIGFPTVVE
KVKVGSAYQYKVKNSKGTTYYITASDKYVDVTGSVKTSSSAPKTTSTSSSSSSIKSVGKIKIVGVSSAAIVMDKPDRNSS
KNIGTVKLGSTISISGSVKGKNNSNGYWEVIYKGKRGYISGQFGSTI

Sequences:

>Translated_367_residues
MSVFTNSYIPVNKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRYFSNAQVYASAHIFVDKAEAICIIPLNEVAYHANDI
QQRDSAGNPYRGVAALKPNANFLSIGVEMCLEKDGSFHSDTVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWV
SNSQKFVDFKNRVKAKMSGKSVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWEDKDVVGTVNKGIGFPTVVE
KVKVGSAYQYKVKNSKGTTYYITASDKYVDVTGSVKTSSSAPKTTSTSSSSSSIKSVGKIKIVGVSSAAIVMDKPDRNSS
KNIGTVKLGSTISISGSVKGKNNSNGYWEVIYKGKRGYISGQFGSTI
>Mature_366_residues
SVFTNSYIPVNKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRYFSNAQVYASAHIFVDKAEAICIIPLNEVAYHANDIQ
QRDSAGNPYRGVAALKPNANFLSIGVEMCLEKDGSFHSDTVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWVS
NSQKFVDFKNRVKAKMSGKSVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWEDKDVVGTVNKGIGFPTVVEK
VKVGSAYQYKVKNSKGTTYYITASDKYVDVTGSVKTSSSAPKTTSTSSSSSSIKSVGKIKIVGVSSAAIVMDKPDRNSSK
NIGTVKLGSTISISGSVKGKNNSNGYWEVIYKGKRGYISGQFGSTI

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Involved in prophage SP-beta-mediated cell lysis

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BLYA_BACSU (O31982)

Other databases:

- EMBL:   AF021803
- EMBL:   AL009126
- RefSeq:   NP_390024.1
- ProteinModelPortal:   O31982
- SMR:   O31982
- EnsemblBacteria:   EBBACT00000000327
- GeneID:   939130
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU21410
- NMPDR:   fig|224308.1.peg.2147
- GenoList:   BSU21410
- GeneTree:   EBGT00050000000335
- ProtClustDB:   CLSK249527
- BioCyc:   BSUB:BSU21410-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002502
- Gene3D:   G3DSA:3.40.80.10
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; SSF55846 Amidase_2

EC number: =3.5.1.28

Molecular weight: Translated: 39629; Mature: 39498

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFTNSYIPVNKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRYFSNAQVYASAHIFVD
CCEECCCEECCCCCCCCCCCHHHHEEEEEEEECCCCCCCCCCHHHHCCCEEEEEEEEEEE
KAEAICIIPLNEVAYHANDIQQRDSAGNPYRGVAALKPNANFLSIGVEMCLEKDGSFHSD
CCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEHHHHHHCCCCCCCH
TVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWVSNSQKFVDFKNRVKAKMSGK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHEECHHHHHHHHHCCC
SVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWEDKDVVGTVNKGIGFPTVVE
CCCCCCCCCCCCCCCCCCCHHCCHHHHHHCCEEECCCCCCCCCCEEEECCCCCCCHHHHH
KVKVGSAYQYKVKNSKGTTYYITASDKYVDVTGSVKTSSSAPKTTSTSSSSSSIKSVGKI
HHHCCCEEEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCEE
KIVGVSSAAIVMDKPDRNSSKNIGTVKLGSTISISGSVKGKNNSNGYWEVIYKGKRGYIS
EEEEECCEEEEEECCCCCCCCCCEEEEECCEEEEEEEECCCCCCCCEEEEEEECCCCEEE
GQFGSTI
CCCCCCC
>Mature Secondary Structure 
SVFTNSYIPVNKYTRPGLKLQGVKKCVLHYTANPGAGADNHRRYFSNAQVYASAHIFVD
CEECCCEECCCCCCCCCCCHHHHEEEEEEEECCCCCCCCCCHHHHCCCEEEEEEEEEEE
KAEAICIIPLNEVAYHANDIQQRDSAGNPYRGVAALKPNANFLSIGVEMCLEKDGSFHSD
CCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEHHHHHHCCCCCCCH
TVERTEDVFVELCNKFGLDPIDDIVRHYDITHKNCPAPWVSNSQKFVDFKNRVKAKMSGK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHEECHHHHHHHHHCCC
SVSKASPTKPTTSSPSSSSAVSGSLKSKVDGLRFYSKPSWEDKDVVGTVNKGIGFPTVVE
CCCCCCCCCCCCCCCCCCCHHCCHHHHHHCCEEECCCCCCCCCCEEEECCCCCCCHHHHH
KVKVGSAYQYKVKNSKGTTYYITASDKYVDVTGSVKTSSSAPKTTSTSSSSSSIKSVGKI
HHHCCCEEEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCEE
KIVGVSSAAIVMDKPDRNSSKNIGTVKLGSTISISGSVKGKNNSNGYWEVIYKGKRGYIS
EEEEECCEEEEEECCCCCCCCCCEEEEECCEEEEEEEECCCCCCCCEEEEEEECCCCEEE
GQFGSTI
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579063; 9384377