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The map label for this gene is cycB [H]

Identifier: 159901075

GI number: 159901075

Start: 5840220

End: 5841539

Strand: Direct

Name: cycB [H]

Synonym: Haur_4563

Alternate gene names: 159901075

Gene position: 5840220-5841539 (Clockwise)

Preceding gene: 159901074

Following gene: 159901076

Centisome position: 92.02

GC content: 51.52

Gene sequence:

>1320_bases
ATGAAGCGTATTTTGAGCTTCCTGCTCGTCAGTATCATGATGGTCGGTTTATTGGCAGCTTGTGGTGGCGAAACTACCCC
AACCACTGCTCCAACCACTGCAGCAGAACCAACCGCCGCCCCAACCACTGCGGCAGAAGCAACCGCTGTTCCAGAAGCAA
CCGCCGCTGCAACTACAGAAGCAACCGCTGCTCCCGAAGCAACTACCGCCCCTGCAACTGGTGGCGACACGATGGCAGCA
ACTGGCGATATCACTTTGTGGCACGCTTACAGCACCGGTGGCGCTGAAGATGCAACCTTAACCGAGTTGATTGAAAAAGC
CAAAGCTGCTTTCCCAGATGCTAACATCAGCGTCTTGCAAGTACCATTCGACCAAGTATTCAGCAAGTTTGAAAATGACG
TTGCTGCTGCTGGCGGCCCAGACTTGCTCTTGGCTCCAAACGATAGCTTGGGCGATTTGGCTCGCAAGAACTTGTTGGCC
GACCTTGATGCTTACAAAGCTAACTTGACCAACATCGCTCCTGCTGGCGTTGCTGGGATGTCGGTTGATGGCAAGCTCTA
TGGTATTCCAGAATCATTCAAAGCGGTTGCTTTGTACTACAACAAATCAACGATTGCCACCCCACCATCAACCACCGACG
AGTTGTTGCAATTGGTCAAAGATGGCAAGAAATTGGTCTTGAACCAAAGCGCTTACCACAACTTTGGCTTCTTCCAAGCT
TTCGGTGCTAGCTTGTTCACCGCTGACAAGGCTTGTGGCTTGGTCAATGGTGGCGGCGATGCCTTGAAGTATTTGCAAGA
TCTCAAAGCTGCTGGCGCAACCTTCTCAACCGATGGTGCTCAAGCTGATGCGCTCTTCCGCGAAGGCCAAGCTGACATGA
TCATCAACGGGCCATGGGTTTTGGCCGACTACCAAGCTGCTTTGAGCGACAAACTTGGTGTTGCCGCAATGCCTGCTGGT
CCTAAGGGTCCTGCTGGTCCATTGACTGGCGTTGACGGTTTCTATGTCAACATCAACAGCCAAAATGTTGAAGGTGCAGT
TGCTTTGGCAATGTACTTGACCAACACCGAATCACAAAAAATCTACACCGAAAAAGCTGGCCACGTTCCAGCTGATGTCA
ACGTTGTACCAACCGATGCGTTGGTGCTCGGCTTCAGCCAAGCTGCTTCAACTGGCTATGCTCGCCCACAAGACCAAGAA
CTTAACAACTTCTGGACTCCAGTTGGCGATGCTGTAACCAAAGCACTTGATGGCGGCGAAGATGCAACCAAGGCGATCAC
TGATGCCTGTGCTGCAATGGATACCGCTAACGGTAAATAA

Upstream 100 bases:

>100_bases
TTGCTGGAGCGTGGCCTAGCGTCACGCGCTACTATTCACCTGTTCGCCCTAATCGGCACGGTAACAGATTTCTCAATATG
GTACAAAGGAGTGTACAGCA

Downstream 100 bases:

>100_bases
GGCAATCAACCGACCCGTTCCTTCCTTGCGAGGGAACGGGTCGAGCCAAGGCTATGGTGGGCTTACCGAAAGATAGTGCT
GTAACGATGCCGTAGTCTGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKRILSFLLVSIMMVGLLAACGGETTPTTAPTTAAEPTAAPTTAAEATAVPEATAAATTEATAAPEATTAPATGGDTMAA
TGDITLWHAYSTGGAEDATLTELIEKAKAAFPDANISVLQVPFDQVFSKFENDVAAAGGPDLLLAPNDSLGDLARKNLLA
DLDAYKANLTNIAPAGVAGMSVDGKLYGIPESFKAVALYYNKSTIATPPSTTDELLQLVKDGKKLVLNQSAYHNFGFFQA
FGASLFTADKACGLVNGGGDALKYLQDLKAAGATFSTDGAQADALFREGQADMIINGPWVLADYQAALSDKLGVAAMPAG
PKGPAGPLTGVDGFYVNINSQNVEGAVALAMYLTNTESQKIYTEKAGHVPADVNVVPTDALVLGFSQAASTGYARPQDQE
LNNFWTPVGDAVTKALDGGEDATKAITDACAAMDTANGK

Sequences:

>Translated_439_residues
MKRILSFLLVSIMMVGLLAACGGETTPTTAPTTAAEPTAAPTTAAEATAVPEATAAATTEATAAPEATTAPATGGDTMAA
TGDITLWHAYSTGGAEDATLTELIEKAKAAFPDANISVLQVPFDQVFSKFENDVAAAGGPDLLLAPNDSLGDLARKNLLA
DLDAYKANLTNIAPAGVAGMSVDGKLYGIPESFKAVALYYNKSTIATPPSTTDELLQLVKDGKKLVLNQSAYHNFGFFQA
FGASLFTADKACGLVNGGGDALKYLQDLKAAGATFSTDGAQADALFREGQADMIINGPWVLADYQAALSDKLGVAAMPAG
PKGPAGPLTGVDGFYVNINSQNVEGAVALAMYLTNTESQKIYTEKAGHVPADVNVVPTDALVLGFSQAASTGYARPQDQE
LNNFWTPVGDAVTKALDGGEDATKAITDACAAMDTANGK
>Mature_439_residues
MKRILSFLLVSIMMVGLLAACGGETTPTTAPTTAAEPTAAPTTAAEATAVPEATAAATTEATAAPEATTAPATGGDTMAA
TGDITLWHAYSTGGAEDATLTELIEKAKAAFPDANISVLQVPFDQVFSKFENDVAAAGGPDLLLAPNDSLGDLARKNLLA
DLDAYKANLTNIAPAGVAGMSVDGKLYGIPESFKAVALYYNKSTIATPPSTTDELLQLVKDGKKLVLNQSAYHNFGFFQA
FGASLFTADKACGLVNGGGDALKYLQDLKAAGATFSTDGAQADALFREGQADMIINGPWVLADYQAALSDKLGVAAMPAG
PKGPAGPLTGVDGFYVNINSQNVEGAVALAMYLTNTESQKIYTEKAGHVPADVNVVPTDALVLGFSQAASTGYARPQDQE
LNNFWTPVGDAVTKALDGGEDATKAITDACAAMDTANGK

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=346, Percent_Identity=28.6127167630058, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44962; Mature: 44962

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILSFLLVSIMMVGLLAACGGETTPTTAPTTAAEPTAAPTTAAEATAVPEATAAATTE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCC
ATAAPEATTAPATGGDTMAATGDITLWHAYSTGGAEDATLTELIEKAKAAFPDANISVLQ
CCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VPFDQVFSKFENDVAAAGGPDLLLAPNDSLGDLARKNLLADLDAYKANLTNIAPAGVAGM
CCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SVDGKLYGIPESFKAVALYYNKSTIATPPSTTDELLQLVKDGKKLVLNQSAYHNFGFFQA
CCCCEEECCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHCCCEEEECCCHHCCCCHHHH
FGASLFTADKACGLVNGGGDALKYLQDLKAAGATFSTDGAQADALFREGQADMIINGPWV
HCHHHHHHHHHCCEECCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHCCCCCEEECCCEE
LADYQAALSDKLGVAAMPAGPKGPAGPLTGVDGFYVNINSQNVEGAVALAMYLTNTESQK
EECHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHEEEEEEEECCCCCE
IYTEKAGHVPADVNVVPTDALVLGFSQAASTGYARPQDQELNNFWTPVGDAVTKALDGGE
EEHHHCCCCCCCCEEECCHHHEEEHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHCCCC
DATKAITDACAAMDTANGK
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRILSFLLVSIMMVGLLAACGGETTPTTAPTTAAEPTAAPTTAAEATAVPEATAAATTE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCC
ATAAPEATTAPATGGDTMAATGDITLWHAYSTGGAEDATLTELIEKAKAAFPDANISVLQ
CCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VPFDQVFSKFENDVAAAGGPDLLLAPNDSLGDLARKNLLADLDAYKANLTNIAPAGVAGM
CCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SVDGKLYGIPESFKAVALYYNKSTIATPPSTTDELLQLVKDGKKLVLNQSAYHNFGFFQA
CCCCEEECCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHCCCEEEECCCHHCCCCHHHH
FGASLFTADKACGLVNGGGDALKYLQDLKAAGATFSTDGAQADALFREGQADMIINGPWV
HCHHHHHHHHHCCEECCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHCCCCCEEECCCEE
LADYQAALSDKLGVAAMPAGPKGPAGPLTGVDGFYVNINSQNVEGAVALAMYLTNTESQK
EECHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHEEEEEEEECCCCCE
IYTEKAGHVPADVNVVPTDALVLGFSQAASTGYARPQDQELNNFWTPVGDAVTKALDGGE
EEHHHCCCCCCCCEEECCHHHEEEHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHCCCC
DATKAITDACAAMDTANGK
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]