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The map label for this gene is gdhA [H]

Identifier: 159901024

GI number: 159901024

Start: 5776297

End: 5777556

Strand: Direct

Name: gdhA [H]

Synonym: Haur_4511

Alternate gene names: 159901024

Gene position: 5776297-5777556 (Clockwise)

Preceding gene: 159901023

Following gene: 159901034

Centisome position: 91.01

GC content: 50.79

Gene sequence:

>1260_bases
ATGGCTGACTCTCATCGCAACGCATTCGAGAATGCTCAGCGTCAATTCGATCTCGCTGCTGAAAAGCTGAATCTCGACCA
CAGCTTGCGTCGAGTTCTGCGCGTCCCTCAACGCGAATTAAGCGTCAATTTCCCTGTCAAAATGGACAATGGTGAGATTC
AAGTGTTTAGCGGTTATCGCGTCCAACACAATGTTTCTCGCGGTCCAGCCAAAGGTGGGATTCGCTACCACCCAGCAGTC
GATATCGATGAAGTTCGTGCTCTCGCGATGTGGATGACCTGGAAGTGTGCGCTGGTTAATATTCCGTATGGTGGCGCAAA
AGGTGGGGTCATTGTAGACCCAACGAAACTATCGCAAAGTGAATTAGAGCGATTAACCCGGCGTTTTGCAACTGAAATTA
GCATTTTGGTTGGCGCTGAAAAAGATATTCCAGCTCCTGATGTTGGAACCAACGGCCAAGTGATGGCTTGGTTTATGGAT
ACGATCTCGATGCATCGTGGCTACACCGTGCCAGCTGTGATCACTGGTAAACCGGTTGAAGTTGGTGGCTCGCTTGGGCG
GGTCGAAGCAACTGGGCGCGGGGTTAGCATTGTGGCCCGCGAAGCCGCCAAACACCTTGGCTTGCGCATCGAAGGCGCAA
CCGTAGTGATTCAGGGCTTTGGTAATGTGGGTAGCGTTACCGCCGATATGATGCAGCGCATGGGCAGCAAAGTGATTGCG
GTTAGCGATGTTTCTGGTGGCTACTACAACCGCCGTGGCCTGAACATCCCCGAAATGATTGCCTATACCAAGCAACATCG
TTCGCTGGAAGGCTACCAAGCCGAAGGCATCGAGCGGGTCAGCAATAACGAGTTGCTCGAAATTGAGTGCGATATTTTGG
CTCCATGTGCCTTGGAAAACCAAATTACCGAGGAAAATGCAGGCCGAATTCGCTGTAAATTGCTGGTCGAAGGCGCAAAC
GGCCCAACTACCCCCGAAGCCGATGATATTTTGTTTGAAAAAGGCATCTTTGTTGTGCCCGATATCTTGGCGAACGCTGG
TGGTGTAACCGTTTCATACTTCGAATGGGTTCAAGGCTTGCAAGAGTTCTTCTGGACTGAAGAAGAAATCAATAACAAGC
TTGAGCGGATTATGCAACACAGCTTTGAATCGGTGCTGGCCCAAACCCTCAAGCATAAAGTTTCAATGCGCATGGGTGGC
TATATGGTCGCAGTAGCCCGGGTCGCTGAAGCAACCCAAATCCGCGGGATTTATCCATAA

Upstream 100 bases:

>100_bases
AACGCCGTTGGATTTATATGTCGTATAATCTTAGAGAGACTTCTGCATAACCAAGAGGCCTCTTTTGATCAAAGGCGATC
AATTAGAAAGGAACAAGTCA

Downstream 100 bases:

>100_bases
ACTAGGCTTTGTGGCCCCTCGTCCAATTGTATGGGCGAGGGGCTTTTTAGTTAAGGATGAAGGATGAATTATGAGGGATG
AAATCAAAAGTAAGAAATAG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MADSHRNAFENAQRQFDLAAEKLNLDHSLRRVLRVPQRELSVNFPVKMDNGEIQVFSGYRVQHNVSRGPAKGGIRYHPAV
DIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPTKLSQSELERLTRRFATEISILVGAEKDIPAPDVGTNGQVMAWFMD
TISMHRGYTVPAVITGKPVEVGGSLGRVEATGRGVSIVAREAAKHLGLRIEGATVVIQGFGNVGSVTADMMQRMGSKVIA
VSDVSGGYYNRRGLNIPEMIAYTKQHRSLEGYQAEGIERVSNNELLEIECDILAPCALENQITEENAGRIRCKLLVEGAN
GPTTPEADDILFEKGIFVVPDILANAGGVTVSYFEWVQGLQEFFWTEEEINNKLERIMQHSFESVLAQTLKHKVSMRMGG
YMVAVARVAEATQIRGIYP

Sequences:

>Translated_419_residues
MADSHRNAFENAQRQFDLAAEKLNLDHSLRRVLRVPQRELSVNFPVKMDNGEIQVFSGYRVQHNVSRGPAKGGIRYHPAV
DIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPTKLSQSELERLTRRFATEISILVGAEKDIPAPDVGTNGQVMAWFMD
TISMHRGYTVPAVITGKPVEVGGSLGRVEATGRGVSIVAREAAKHLGLRIEGATVVIQGFGNVGSVTADMMQRMGSKVIA
VSDVSGGYYNRRGLNIPEMIAYTKQHRSLEGYQAEGIERVSNNELLEIECDILAPCALENQITEENAGRIRCKLLVEGAN
GPTTPEADDILFEKGIFVVPDILANAGGVTVSYFEWVQGLQEFFWTEEEINNKLERIMQHSFESVLAQTLKHKVSMRMGG
YMVAVARVAEATQIRGIYP
>Mature_418_residues
ADSHRNAFENAQRQFDLAAEKLNLDHSLRRVLRVPQRELSVNFPVKMDNGEIQVFSGYRVQHNVSRGPAKGGIRYHPAVD
IDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPTKLSQSELERLTRRFATEISILVGAEKDIPAPDVGTNGQVMAWFMDT
ISMHRGYTVPAVITGKPVEVGGSLGRVEATGRGVSIVAREAAKHLGLRIEGATVVIQGFGNVGSVTADMMQRMGSKVIAV
SDVSGGYYNRRGLNIPEMIAYTKQHRSLEGYQAEGIERVSNNELLEIECDILAPCALENQITEENAGRIRCKLLVEGANG
PTTPEADDILFEKGIFVVPDILANAGGVTVSYFEWVQGLQEFFWTEEEINNKLERIMQHSFESVLAQTLKHKVSMRMGGY
MVAVARVAEATQIRGIYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=383, Percent_Identity=41.77545691906, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4885281, Length=382, Percent_Identity=41.3612565445026, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI1788059, Length=359, Percent_Identity=32.5905292479109, Blast_Score=184, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=389, Percent_Identity=40.1028277634961, Blast_Score=276, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6324951, Length=412, Percent_Identity=29.8543689320388, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319256, Length=394, Percent_Identity=30.7106598984772, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24649283, Length=334, Percent_Identity=43.4131736526946, Blast_Score=278, Evalue=7e-75,
Organism=Drosophila melanogaster, GI45549226, Length=380, Percent_Identity=39.4736842105263, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI45553475, Length=363, Percent_Identity=40.771349862259, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649460, Length=328, Percent_Identity=39.3292682926829, Blast_Score=225, Evalue=4e-59,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46161; Mature: 46029

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSHRNAFENAQRQFDLAAEKLNLDHSLRRVLRVPQRELSVNFPVKMDNGEIQVFSGYR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCEEECCEEECCCCEEEEECEE
VQHNVSRGPAKGGIRYHPAVDIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPTKLSQS
EEECCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCEEECCCCCCHH
ELERLTRRFATEISILVGAEKDIPAPDVGTNGQVMAWFMDTISMHRGYTVPAVITGKPVE
HHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCEE
VGGSLGRVEATGRGVSIVAREAAKHLGLRIEGATVVIQGFGNVGSVTADMMQRMGSKVIA
CCCCCCEEEECCCCHHHHHHHHHHHCCEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEE
VSDVSGGYYNRRGLNIPEMIAYTKQHRSLEGYQAEGIERVSNNELLEIECDILAPCALEN
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCHHHCCCCCEEEEEECEECCCHHCC
QITEENAGRIRCKLLVEGANGPTTPEADDILFEKGIFVVPDILANAGGVTVSYFEWVQGL
CCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCEEECHHHCCCCCCEEHHHHHHHHH
QEFFWTEEEINNKLERIMQHSFESVLAQTLKHKVSMRMGGYMVAVARVAEATQIRGIYP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADSHRNAFENAQRQFDLAAEKLNLDHSLRRVLRVPQRELSVNFPVKMDNGEIQVFSGYR
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCEEECCEEECCCCEEEEECEE
VQHNVSRGPAKGGIRYHPAVDIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPTKLSQS
EEECCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCEEECCCCCCHH
ELERLTRRFATEISILVGAEKDIPAPDVGTNGQVMAWFMDTISMHRGYTVPAVITGKPVE
HHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCEE
VGGSLGRVEATGRGVSIVAREAAKHLGLRIEGATVVIQGFGNVGSVTADMMQRMGSKVIA
CCCCCCEEEECCCCHHHHHHHHHHHCCEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEE
VSDVSGGYYNRRGLNIPEMIAYTKQHRSLEGYQAEGIERVSNNELLEIECDILAPCALEN
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCHHHCCCCCEEEEEECEECCCHHCC
QITEENAGRIRCKLLVEGANGPTTPEADDILFEKGIFVVPDILANAGGVTVSYFEWVQGL
CCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCEEECHHHCCCCCCEEHHHHHHHHH
QEFFWTEEEINNKLERIMQHSFESVLAQTLKHKVSMRMGGYMVAVARVAEATQIRGIYP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]