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The map label for this gene is pfkA [H]

Identifier: 159901012

GI number: 159901012

Start: 5759585

End: 5760676

Strand: Direct

Name: pfkA [H]

Synonym: Haur_4499

Alternate gene names: 159901012

Gene position: 5759585-5760676 (Clockwise)

Preceding gene: 159901009

Following gene: 159901013

Centisome position: 90.75

GC content: 52.2

Gene sequence:

>1092_bases
ATGGCCATTCGCAAAGTTGCAATTTTAACCAGTGGTGGCGATGCCCCAGGCTTAAACGGTGTTATTCGGGCTTTTGTAAA
ATCGGCAGTTTTAGAGCATGGCTGGGAAGTGGTGGGGATTGAAGATGGTTTTGAGGGTTTGGTTGGTACGCCGCGCCTGC
GGCCTTTAGGATTGAAAGAAGTGCGTGGGTTGCTCGGTCGCGGCGGCACGATTTTGGGCAGCACCAACAAAGGTAATTTT
GGTTGGCACAAAAATGCCGATGGCGAGCTGGTGCGCAACGATGCATCCTACCAAGAATTTGTGGCCAATGCCAAAGCTGA
AGGTATCGATGCAGTCGTGGTCTTAGGCGGCGAAGGTAGTCAATCGATCGCCCACGATTTGGGGAGCTTTGGTTTGCCAG
TGGTTGGCGTGCCCAAAACGATCGATAACGATTTGGTGGGTACAGACCAAACCTTCGGTTTTGATACAGCGATTGGGGTT
GCTACCGATGCGCTTGATCGCTTGCACACCACCGCCGAAAGCCATGATCGGGTGATGGTGCTCGAAGTCATGGGTCGCCA
TGCGGGCTGGATTGCCTTGCACGCTGGCTTGGCTGGCGGGGTTGATGCCATCCTGATTCCTGAAATTCCCTTCGATTTAC
GCGCTCTGGCCCACAAAATTGGCGAACGTGATGCATTTGGTTCATCGTTTAGCATCATTATCGTGGCTGAGGGCGCAATG
CCAGCCGGCGGCGAGGCAATTTACCAACGCGAAGGCTTGCTCGGCGGGATTGGCTATTACGTTGCCAATCAATTGGCTGA
ATTAACTGGCAAAGATACCCGGGCAGTCGTGTTGGGGCATATTCAACGTGGTGGCTCACCTACGCCGATCGATCGAATTT
TGGCTTCACGCTATGGAGTTGGCGCAGTTTGGGCGATCTCTCAAGGGCGCTTTGGCCAACTAGTGGCAATCCAAAACGAT
CAATTGATTACCGTGCCATTGCTTGAATGTGCCAACAAAATTAAGCCTGTGCCAGTTGATGGCCAAATGGTACGAACTGG
CCGTTCGTTGGGCATTGTGTTTGGCGATGAAGAACAACGAATTAATCAATAG

Upstream 100 bases:

>100_bases
CGTATGAATATTCCTTGCCAGAGCCAACCTGATAGTGCTAGGATAGCCATAATTAATCGGGCTTGATTTCATGCAGATAT
AGATATTAAGGAGTTGTGCC

Downstream 100 bases:

>100_bases
GTTTAACGTTATGCATTTTCAATCCATACATAACTGCTTTTATGGTTCGCCACCCTTCCGTCCCTCCGCCGGAGGGAAAC
GCAGGGGGGGGTCAGCCCCC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAIRKVAILTSGGDAPGLNGVIRAFVKSAVLEHGWEVVGIEDGFEGLVGTPRLRPLGLKEVRGLLGRGGTILGSTNKGNF
GWHKNADGELVRNDASYQEFVANAKAEGIDAVVVLGGEGSQSIAHDLGSFGLPVVGVPKTIDNDLVGTDQTFGFDTAIGV
ATDALDRLHTTAESHDRVMVLEVMGRHAGWIALHAGLAGGVDAILIPEIPFDLRALAHKIGERDAFGSSFSIIIVAEGAM
PAGGEAIYQREGLLGGIGYYVANQLAELTGKDTRAVVLGHIQRGGSPTPIDRILASRYGVGAVWAISQGRFGQLVAIQND
QLITVPLLECANKIKPVPVDGQMVRTGRSLGIVFGDEEQRINQ

Sequences:

>Translated_363_residues
MAIRKVAILTSGGDAPGLNGVIRAFVKSAVLEHGWEVVGIEDGFEGLVGTPRLRPLGLKEVRGLLGRGGTILGSTNKGNF
GWHKNADGELVRNDASYQEFVANAKAEGIDAVVVLGGEGSQSIAHDLGSFGLPVVGVPKTIDNDLVGTDQTFGFDTAIGV
ATDALDRLHTTAESHDRVMVLEVMGRHAGWIALHAGLAGGVDAILIPEIPFDLRALAHKIGERDAFGSSFSIIIVAEGAM
PAGGEAIYQREGLLGGIGYYVANQLAELTGKDTRAVVLGHIQRGGSPTPIDRILASRYGVGAVWAISQGRFGQLVAIQND
QLITVPLLECANKIKPVPVDGQMVRTGRSLGIVFGDEEQRINQ
>Mature_362_residues
AIRKVAILTSGGDAPGLNGVIRAFVKSAVLEHGWEVVGIEDGFEGLVGTPRLRPLGLKEVRGLLGRGGTILGSTNKGNFG
WHKNADGELVRNDASYQEFVANAKAEGIDAVVVLGGEGSQSIAHDLGSFGLPVVGVPKTIDNDLVGTDQTFGFDTAIGVA
TDALDRLHTTAESHDRVMVLEVMGRHAGWIALHAGLAGGVDAILIPEIPFDLRALAHKIGERDAFGSSFSIIIVAEGAMP
AGGEAIYQREGLLGGIGYYVANQLAELTGKDTRAVVLGHIQRGGSPTPIDRILASRYGVGAVWAISQGRFGQLVAIQNDQ
LITVPLLECANKIKPVPVDGQMVRTGRSLGIVFGDEEQRINQ

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=364, Percent_Identity=35.989010989011, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI266453748, Length=364, Percent_Identity=35.989010989011, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI4505749, Length=364, Percent_Identity=35.989010989011, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI266453619, Length=364, Percent_Identity=35.989010989011, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI48762920, Length=364, Percent_Identity=34.6153846153846, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI11321601, Length=364, Percent_Identity=34.6153846153846, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1790350, Length=348, Percent_Identity=39.6551724137931, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI25147584, Length=364, Percent_Identity=32.6923076923077, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17558788, Length=331, Percent_Identity=30.8157099697885, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI72003601, Length=193, Percent_Identity=36.7875647668394, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321679, Length=370, Percent_Identity=34.0540540540541, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323861, Length=366, Percent_Identity=32.5136612021858, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652337, Length=362, Percent_Identity=33.9779005524862, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI17647809, Length=362, Percent_Identity=33.9779005524862, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28573326, Length=362, Percent_Identity=33.1491712707182, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38161; Mature: 38030

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRKVAILTSGGDAPGLNGVIRAFVKSAVLEHGWEVVGIEDGFEGLVGTPRLRPLGLKE
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHH
VRGLLGRGGTILGSTNKGNFGWHKNADGELVRNDASYQEFVANAKAEGIDAVVVLGGEGS
HHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCEEEEECCCCC
QSIAHDLGSFGLPVVGVPKTIDNDLVGTDQTFGFDTAIGVATDALDRLHTTAESHDRVMV
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
LEVMGRHAGWIALHAGLAGGVDAILIPEIPFDLRALAHKIGERDAFGSSFSIIIVAEGAM
EEECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
PAGGEAIYQREGLLGGIGYYVANQLAELTGKDTRAVVLGHIQRGGSPTPIDRILASRYGV
CCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCHHHHHHHHHCCC
GAVWAISQGRFGQLVAIQNDQLITVPLLECANKIKPVPVDGQMVRTGRSLGIVFGDEEQR
CEEEEECCCCCEEEEEECCCCEEEEEHHHHHHHCCCCCCCCCEEECCCEEEEEECCHHHH
INQ
CCC
>Mature Secondary Structure 
AIRKVAILTSGGDAPGLNGVIRAFVKSAVLEHGWEVVGIEDGFEGLVGTPRLRPLGLKE
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHH
VRGLLGRGGTILGSTNKGNFGWHKNADGELVRNDASYQEFVANAKAEGIDAVVVLGGEGS
HHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCEEEEECCCCC
QSIAHDLGSFGLPVVGVPKTIDNDLVGTDQTFGFDTAIGVATDALDRLHTTAESHDRVMV
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
LEVMGRHAGWIALHAGLAGGVDAILIPEIPFDLRALAHKIGERDAFGSSFSIIIVAEGAM
EEECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
PAGGEAIYQREGLLGGIGYYVANQLAELTGKDTRAVVLGHIQRGGSPTPIDRILASRYGV
CCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCHHHHHHHHHCCC
GAVWAISQGRFGQLVAIQNDQLITVPLLECANKIKPVPVDGQMVRTGRSLGIVFGDEEQR
CEEEEECCCCCEEEEEECCCCEEEEEHHHHHHHCCCCCCCCCEEECCCEEEEEECCHHHH
INQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA