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The map label for this gene is pleD [H]

Identifier: 159899942

GI number: 159899942

Start: 4314818

End: 4315492

Strand: Direct

Name: pleD [H]

Synonym: Haur_3425

Alternate gene names: 159899942

Gene position: 4314818-4315492 (Clockwise)

Preceding gene: 159899941

Following gene: 159899943

Centisome position: 67.99

GC content: 50.96

Gene sequence:

>675_bases
ATGACATTTCCCCAAACCTTTGAACTAGCCACAACCGACAAACTAACGGGCCTTTTTACGCCTGATTACTTTCGCTTTTT
GGTGCGCGAACAAGTGTTGCCAGCCATGCACGAACGGGTTGAACCCTTGAGCATGTTTGTGTTGGATATCGATTATTTTC
TAGCCTTGAATACTGAATCAGGCCGTGAATGTGGCGATGCAGTGTTAGTTGCCGTGGCCCAAGTGCTCAAGCGGGTGTTG
CCAGAGCAAGCAATGCTCTCGCGCTACAGTGGCGATGAGTTTGCCGGAGCGTTGCCTGAACTACGAATCGACGACGCATT
TTCGTTGCTCGAAGAAGTGCGCCGTCAAGTGTTGGCAATTCAGCTTGATTGCCTTGGCGAAATTAAATTAAGCTGTTCGA
TCGGCTTGGCTGGCTATCCAGGCCATGGCCGCGATGAAGTTGAGTTGGTGCGTCAGGCCGACGAAGCGCTGTATCACGCC
AAAACAACAGGCCGCAACAAAATTGCCCTGCCCGTCGCCGATAGCCGTATGATCACCAAAACCAGCTACTACACTGCCAC
CCAACTCGAACGTTTAGCCCAACTTGCCAAAGCCCTCAAACGCAACGAAGCTAGTTTGCTGCGCGAAGCGCTCGACGATG
TGATCAAAAAATACAACGATCGCTTGAAATCCTAA

Upstream 100 bases:

>100_bases
AAGCCACAGGCCCAAACCTGTGGCTTTTTTGTATTGACAAAACGCCACATATTATGCTAAACATACATATATTATTAAAC
ATATGTATATTGGAGAACAT

Downstream 100 bases:

>100_bases
ACAAGGCTTGACACTTTGAAACATTTGTTCTATATTTTTTATGTAGATTGACGGATACGGGCCAGCAATGGCTATGGGTA
ACCGAGTTCATGAAAGGAAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTFPQTFELATTDKLTGLFTPDYFRFLVREQVLPAMHERVEPLSMFVLDIDYFLALNTESGRECGDAVLVAVAQVLKRVL
PEQAMLSRYSGDEFAGALPELRIDDAFSLLEEVRRQVLAIQLDCLGEIKLSCSIGLAGYPGHGRDEVELVRQADEALYHA
KTTGRNKIALPVADSRMITKTSYYTATQLERLAQLAKALKRNEASLLREALDDVIKKYNDRLKS

Sequences:

>Translated_224_residues
MTFPQTFELATTDKLTGLFTPDYFRFLVREQVLPAMHERVEPLSMFVLDIDYFLALNTESGRECGDAVLVAVAQVLKRVL
PEQAMLSRYSGDEFAGALPELRIDDAFSLLEEVRRQVLAIQLDCLGEIKLSCSIGLAGYPGHGRDEVELVRQADEALYHA
KTTGRNKIALPVADSRMITKTSYYTATQLERLAQLAKALKRNEASLLREALDDVIKKYNDRLKS
>Mature_223_residues
TFPQTFELATTDKLTGLFTPDYFRFLVREQVLPAMHERVEPLSMFVLDIDYFLALNTESGRECGDAVLVAVAQVLKRVLP
EQAMLSRYSGDEFAGALPELRIDDAFSLLEEVRRQVLAIQLDCLGEIKLSCSIGLAGYPGHGRDEVELVRQADEALYHAK
TTGRNKIALPVADSRMITKTSYYTATQLERLAQLAKALKRNEASLLREALDDVIKKYNDRLKS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=134, Percent_Identity=36.5671641791045, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=142, Percent_Identity=32.3943661971831, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=30.8176100628931, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=29.585798816568, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=26.875, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=31.9277108433735, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 25188; Mature: 25057

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPQTFELATTDKLTGLFTPDYFRFLVREQVLPAMHERVEPLSMFVLDIDYFLALNTES
CCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCC
GRECGDAVLVAVAQVLKRVLPEQAMLSRYSGDEFAGALPELRIDDAFSLLEEVRRQVLAI
CHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
QLDCLGEIKLSCSIGLAGYPGHGRDEVELVRQADEALYHAKTTGRNKIALPVADSRMITK
HHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEH
TSYYTATQLERLAQLAKALKRNEASLLREALDDVIKKYNDRLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFPQTFELATTDKLTGLFTPDYFRFLVREQVLPAMHERVEPLSMFVLDIDYFLALNTES
CCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCC
GRECGDAVLVAVAQVLKRVLPEQAMLSRYSGDEFAGALPELRIDDAFSLLEEVRRQVLAI
CHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
QLDCLGEIKLSCSIGLAGYPGHGRDEVELVRQADEALYHAKTTGRNKIALPVADSRMITK
HHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEH
TSYYTATQLERLAQLAKALKRNEASLLREALDDVIKKYNDRLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA