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The map label for this gene is hemL [H]

Identifier: 159899674

GI number: 159899674

Start: 3984425

End: 3985717

Strand: Direct

Name: hemL [H]

Synonym: Haur_3156

Alternate gene names: 159899674

Gene position: 3984425-3985717 (Clockwise)

Preceding gene: 159899673

Following gene: 159899677

Centisome position: 62.78

GC content: 49.88

Gene sequence:

>1293_bases
ATGAGTGCAGCACAACTGCCCAAGGTCAGCGGCGAAGCCCTAAATCTCACCAAATCGATGGAATTGTTAGTCCAAGCCGA
AGCGATTGTTCCAGGAACAACCCAATCGTTGATGAAACGGCCTGAGCAATTTGCCTATGGTTCGTTTCCAGTTTTTATTG
ATCATGGCGATGGCGCGTTGGTAACCGATGTTGATGGCAATCAATATATCGATTTTATTTGTGGCTTGGGTGCAACCACC
TTGGGTCACAACCATCTAGCAGTGGTCGAGGCAATTCGCAATAACTTGGATAAAGGGCTGATTCATTCGCTGCCCACCGA
AGTTGAGCTACGCGCAACTCAAGCATTGATCGATATTATTCCGAATGCTGAAATGGCTCGCTTCTTCAAAACTGGGGCTG
ATGCCACTTCGGCTGCTGTGCGTTTGGCTCGCCACCTGACCAAGCGCGAACGGATTATCACGGTCGGCTACAATGGTTGG
CACGACCATTTTATGTATTACACTCCTGGCGTGCCCGCAGTGCTCAGCCAATACACCGAACAAGTATCGTTGATGGCTCC
CCACGAAAAGCCCAACTTGATCGCTGCCATCAACAACCATGGCGATCAATTGGCCGCCGTGTTGCTTTCAATGCCCTACA
AACACTGCTTGGATGCTGAGTATTTGAACGAAGTTAAGGCTGCTTGTCATGCAGTTGGCGCATTATTTGTGCTCGATGAA
GTGGTAACGGGCTTCCGCTTGGCGTTGGGTGGGGCGCAAGAATTCTATGGCGTTGATGCAGATTTCGTCTGTCTCTCAAA
GGGCATCGCCGCAGGTATGCCACTTTCTGCGATTACTGGACCCAAAAAGCATCTCGAACGCTTATCCGATTTGCAAGTTT
CAACCACCTTTGGCGGCGAAATGCTCTCGCTGGAAGTTTGCTACGAAGTGATTAACATCTATCGCAACACTAACTATTTT
GAGCATGTGGCCAAGCTTGGTCAACGCTTGCGTGAAGGCGTGAACGCCAAAGCTGAGGCGCTTGGAGTGGCATTGCGCGT
CTGTGGCTATGATGCAATTCCCTTCTTCTCCTTTGCACCTGATATGCCAACCCATGCTCGTTTGATGGAAGCCTTGTTGG
GCACATTGGCCAAACGTGGAGTCATTTTGCGTCGCGATGTCAACTTCTTGACCAGCGCCCATACAGTTGAGCAAATTGAT
TTCACGATCGAGGCTGTCGCCCAAGGTTTACAAGAATTGCTTGATCGCGGCATTATCGAATCGACCAACGGCAAAGAACA
AGCTGCTGGCTAA

Upstream 100 bases:

>100_bases
TACTAGCGCTCAAATAAAATCTATTTAATCCGACTAAATTGATTTTATTCTACATAATCATATAACCAAACGTCGAGCAT
ATATGGAAGGATCGAGCAGC

Downstream 100 bases:

>100_bases
ATTGATTGATCCACGAAGGACACGAAGGGCACGAAATTAAGGATTAGCGTTACTAAGAGCAATTCGCAATCTATAAAACA
GAGGTGCAGGGGCTATTTTC

Product: class III aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSAAQLPKVSGEALNLTKSMELLVQAEAIVPGTTQSLMKRPEQFAYGSFPVFIDHGDGALVTDVDGNQYIDFICGLGATT
LGHNHLAVVEAIRNNLDKGLIHSLPTEVELRATQALIDIIPNAEMARFFKTGADATSAAVRLARHLTKRERIITVGYNGW
HDHFMYYTPGVPAVLSQYTEQVSLMAPHEKPNLIAAINNHGDQLAAVLLSMPYKHCLDAEYLNEVKAACHAVGALFVLDE
VVTGFRLALGGAQEFYGVDADFVCLSKGIAAGMPLSAITGPKKHLERLSDLQVSTTFGGEMLSLEVCYEVINIYRNTNYF
EHVAKLGQRLREGVNAKAEALGVALRVCGYDAIPFFSFAPDMPTHARLMEALLGTLAKRGVILRRDVNFLTSAHTVEQID
FTIEAVAQGLQELLDRGIIESTNGKEQAAG

Sequences:

>Translated_430_residues
MSAAQLPKVSGEALNLTKSMELLVQAEAIVPGTTQSLMKRPEQFAYGSFPVFIDHGDGALVTDVDGNQYIDFICGLGATT
LGHNHLAVVEAIRNNLDKGLIHSLPTEVELRATQALIDIIPNAEMARFFKTGADATSAAVRLARHLTKRERIITVGYNGW
HDHFMYYTPGVPAVLSQYTEQVSLMAPHEKPNLIAAINNHGDQLAAVLLSMPYKHCLDAEYLNEVKAACHAVGALFVLDE
VVTGFRLALGGAQEFYGVDADFVCLSKGIAAGMPLSAITGPKKHLERLSDLQVSTTFGGEMLSLEVCYEVINIYRNTNYF
EHVAKLGQRLREGVNAKAEALGVALRVCGYDAIPFFSFAPDMPTHARLMEALLGTLAKRGVILRRDVNFLTSAHTVEQID
FTIEAVAQGLQELLDRGIIESTNGKEQAAG
>Mature_429_residues
SAAQLPKVSGEALNLTKSMELLVQAEAIVPGTTQSLMKRPEQFAYGSFPVFIDHGDGALVTDVDGNQYIDFICGLGATTL
GHNHLAVVEAIRNNLDKGLIHSLPTEVELRATQALIDIIPNAEMARFFKTGADATSAAVRLARHLTKRERIITVGYNGWH
DHFMYYTPGVPAVLSQYTEQVSLMAPHEKPNLIAAINNHGDQLAAVLLSMPYKHCLDAEYLNEVKAACHAVGALFVLDEV
VTGFRLALGGAQEFYGVDADFVCLSKGIAAGMPLSAITGPKKHLERLSDLQVSTTFGGEMLSLEVCYEVINIYRNTNYFE
HVAKLGQRLREGVNAKAEALGVALRVCGYDAIPFFSFAPDMPTHARLMEALLGTLAKRGVILRRDVNFLTSAHTVEQIDF
TIEAVAQGLQELLDRGIIESTNGKEQAAG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=330, Percent_Identity=24.5454545454545, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=433, Percent_Identity=30.0230946882217, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789016, Length=388, Percent_Identity=25.2577319587629, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789759, Length=294, Percent_Identity=25.8503401360544, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=341, Percent_Identity=23.7536656891496, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46709; Mature: 46578

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00435 PEROXIDASE_1 ; PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAQLPKVSGEALNLTKSMELLVQAEAIVPGTTQSLMKRPEQFAYGSFPVFIDHGDGAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHCCCCCCEEEECCCCEE
VTDVDGNQYIDFICGLGATTLGHNHLAVVEAIRNNLDKGLIHSLPTEVELRATQALIDII
EEECCCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
PNAEMARFFKTGADATSAAVRLARHLTKRERIITVGYNGWHDHFMYYTPGVPAVLSQYTE
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECCCHHHHHHHHHH
QVSLMAPHEKPNLIAAINNHGDQLAAVLLSMPYKHCLDAEYLNEVKAACHAVGALFVLDE
HHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VVTGFRLALGGAQEFYGVDADFVCLSKGIAAGMPLSAITGPKKHLERLSDLQVSTTFGGE
HHHHHHHHHCCCHHHHCCCCCHHHHHCCHHCCCCHHHCCCCHHHHHHHHCCEEEEECCCH
MLSLEVCYEVINIYRNTNYFEHVAKLGQRLREGVNAKAEALGVALRVCGYDAIPFFSFAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DMPTHARLMEALLGTLAKRGVILRRDVNFLTSAHTVEQIDFTIEAVAQGLQELLDRGIIE
CCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
STNGKEQAAG
CCCCCCCCCC
>Mature Secondary Structure 
SAAQLPKVSGEALNLTKSMELLVQAEAIVPGTTQSLMKRPEQFAYGSFPVFIDHGDGAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHCCCCCCEEEECCCCEE
VTDVDGNQYIDFICGLGATTLGHNHLAVVEAIRNNLDKGLIHSLPTEVELRATQALIDII
EEECCCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
PNAEMARFFKTGADATSAAVRLARHLTKRERIITVGYNGWHDHFMYYTPGVPAVLSQYTE
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECCCHHHHHHHHHH
QVSLMAPHEKPNLIAAINNHGDQLAAVLLSMPYKHCLDAEYLNEVKAACHAVGALFVLDE
HHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VVTGFRLALGGAQEFYGVDADFVCLSKGIAAGMPLSAITGPKKHLERLSDLQVSTTFGGE
HHHHHHHHHCCCHHHHCCCCCHHHHHCCHHCCCCHHHCCCCHHHHHHHHCCEEEEECCCH
MLSLEVCYEVINIYRNTNYFEHVAKLGQRLREGVNAKAEALGVALRVCGYDAIPFFSFAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DMPTHARLMEALLGTLAKRGVILRRDVNFLTSAHTVEQIDFTIEAVAQGLQELLDRGIIE
CCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
STNGKEQAAG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA